Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14015 and SGL_RS14020 overlap by 4 nucleotides SGL_RS14020 and SGL_RS14025 are separated by 21 nucleotides SGL_RS14025 and SGL_RS14030 are separated by 454 nucleotides
SGL_RS14015: SGL_RS14015 - AI-2E family transporter, at 2,604,794 to 2,605,873
_RS14015
SGL_RS14020: SGL_RS14020 - hypothetical protein, at 2,605,870 to 2,606,073
_RS14020
SGL_RS14025: SGL_RS14025 - gamma-glutamyl-phosphate reductase, at 2,606,095 to 2,607,357
_RS14025
SGL_RS14030: SGL_RS14030 - ATP-binding protein, at 2,607,812 to 2,610,058
_RS14030
Position (kb)
2606
2607
2608 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2605.192 kb on + strand, within SGL_RS14015 at 2605.193 kb on + strand, within SGL_RS14015 at 2605.374 kb on + strand, within SGL_RS14015 at 2605.374 kb on + strand, within SGL_RS14015 at 2605.382 kb on - strand, within SGL_RS14015 at 2605.429 kb on + strand, within SGL_RS14015 at 2605.430 kb on + strand, within SGL_RS14015 at 2605.438 kb on - strand, within SGL_RS14015 at 2605.443 kb on - strand, within SGL_RS14015 at 2605.444 kb on - strand, within SGL_RS14015 at 2605.444 kb on - strand, within SGL_RS14015 at 2605.491 kb on + strand, within SGL_RS14015 at 2605.499 kb on - strand, within SGL_RS14015 at 2605.576 kb on + strand, within SGL_RS14015 at 2605.610 kb on + strand, within SGL_RS14015 at 2605.618 kb on - strand, within SGL_RS14015 at 2605.689 kb on - strand, within SGL_RS14015 at 2605.701 kb on - strand, within SGL_RS14015 at 2605.775 kb on - strand at 2605.952 kb on + strand, within SGL_RS14020 at 2605.965 kb on - strand, within SGL_RS14020 at 2606.097 kb on + strand at 2606.271 kb on - strand, within SGL_RS14025 at 2606.330 kb on + strand, within SGL_RS14025 at 2606.425 kb on + strand, within SGL_RS14025 at 2606.484 kb on - strand, within SGL_RS14025 at 2606.598 kb on - strand, within SGL_RS14025 at 2606.598 kb on - strand, within SGL_RS14025 at 2606.654 kb on + strand, within SGL_RS14025 at 2606.693 kb on + strand, within SGL_RS14025 at 2606.784 kb on - strand, within SGL_RS14025 at 2606.850 kb on - strand, within SGL_RS14025 at 2606.913 kb on - strand, within SGL_RS14025 at 2607.027 kb on - strand, within SGL_RS14025 at 2607.187 kb on + strand, within SGL_RS14025 at 2607.187 kb on + strand, within SGL_RS14025 at 2607.187 kb on + strand, within SGL_RS14025 at 2607.187 kb on + strand, within SGL_RS14025 at 2607.195 kb on - strand, within SGL_RS14025 at 2607.195 kb on - strand, within SGL_RS14025 at 2607.202 kb on - strand, within SGL_RS14025 at 2607.252 kb on - strand at 2607.252 kb on - strand at 2607.264 kb on + strand at 2607.714 kb on - strand at 2607.796 kb on + strand at 2607.853 kb on + strand at 2607.890 kb on - strand at 2607.900 kb on + strand at 2607.900 kb on - strand at 2607.908 kb on - strand at 2608.039 kb on + strand, within SGL_RS14030 at 2608.072 kb on - strand, within SGL_RS14030 at 2608.183 kb on + strand, within SGL_RS14030 at 2608.183 kb on + strand, within SGL_RS14030 at 2608.191 kb on - strand, within SGL_RS14030 at 2608.228 kb on + strand, within SGL_RS14030 at 2608.238 kb on + strand, within SGL_RS14030 at 2608.354 kb on + strand, within SGL_RS14030
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,605,192 + SGL_RS14015 0.37 +2.0 2,605,193 + SGL_RS14015 0.37 +1.2 2,605,374 + SGL_RS14015 0.54 +1.6 2,605,374 + SGL_RS14015 0.54 +1.5 2,605,382 - SGL_RS14015 0.54 +1.5 2,605,429 + SGL_RS14015 0.59 +0.6 2,605,430 + SGL_RS14015 0.59 +2.4 2,605,438 - SGL_RS14015 0.60 +2.5 2,605,443 - SGL_RS14015 0.60 +1.6 2,605,444 - SGL_RS14015 0.60 +1.8 2,605,444 - SGL_RS14015 0.60 +1.0 2,605,491 + SGL_RS14015 0.65 +1.6 2,605,499 - SGL_RS14015 0.65 +1.8 2,605,576 + SGL_RS14015 0.72 +1.6 2,605,610 + SGL_RS14015 0.76 +1.9 2,605,618 - SGL_RS14015 0.76 +1.9 2,605,689 - SGL_RS14015 0.83 +1.3 2,605,701 - SGL_RS14015 0.84 +2.0 2,605,775 - +2.4 2,605,952 + SGL_RS14020 0.40 +1.3 2,605,965 - SGL_RS14020 0.47 -0.0 2,606,097 + -3.8 2,606,271 - SGL_RS14025 0.14 -4.6 2,606,330 + SGL_RS14025 0.19 -3.4 2,606,425 + SGL_RS14025 0.26 -4.0 2,606,484 - SGL_RS14025 0.31 -3.5 2,606,598 - SGL_RS14025 0.40 -4.4 2,606,598 - SGL_RS14025 0.40 -6.1 2,606,654 + SGL_RS14025 0.44 -3.0 2,606,693 + SGL_RS14025 0.47 -5.2 2,606,784 - SGL_RS14025 0.55 -3.7 2,606,850 - SGL_RS14025 0.60 -5.1 2,606,913 - SGL_RS14025 0.65 -3.4 2,607,027 - SGL_RS14025 0.74 -5.8 2,607,187 + SGL_RS14025 0.86 -4.4 2,607,187 + SGL_RS14025 0.86 -6.1 2,607,187 + SGL_RS14025 0.86 -2.4 2,607,187 + SGL_RS14025 0.86 -4.5 2,607,195 - SGL_RS14025 0.87 -4.0 2,607,195 - SGL_RS14025 0.87 -4.1 2,607,202 - SGL_RS14025 0.88 -2.7 2,607,252 - -4.5 2,607,252 - -3.9 2,607,264 + -3.3 2,607,714 - -0.6 2,607,796 + -0.1 2,607,853 + -0.1 2,607,890 - +0.0 2,607,900 + -0.1 2,607,900 - +0.1 2,607,908 - -0.3 2,608,039 + SGL_RS14030 0.10 -0.0 2,608,072 - SGL_RS14030 0.12 -0.2 2,608,183 + SGL_RS14030 0.17 -1.5 2,608,183 + SGL_RS14030 0.17 +0.3 2,608,191 - SGL_RS14030 0.17 +0.2 2,608,228 + SGL_RS14030 0.19 -0.3 2,608,238 + SGL_RS14030 0.19 +1.9 2,608,354 + SGL_RS14030 0.24 +0.4
Or see this region's nucleotide sequence