Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13530 and SGL_RS13535 are separated by 114 nucleotides SGL_RS13535 and SGL_RS13540 are separated by 127 nucleotides SGL_RS13540 and SGL_RS13545 are separated by 238 nucleotides
SGL_RS13530: SGL_RS13530 - undecaprenyl-diphosphate phosphatase, at 2,507,382 to 2,508,362
_RS13530
SGL_RS13535: SGL_RS13535 - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 2,508,477 to 2,509,034
_RS13535
SGL_RS13540: SGL_RS13540 - hypothetical protein, at 2,509,162 to 2,509,584
_RS13540
SGL_RS13545: SGL_RS13545 - hypothetical protein, at 2,509,823 to 2,510,014
_RS13545
Position (kb)
2508
2509
2510 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2508.313 kb on + strand at 2508.405 kb on - strand at 2508.693 kb on + strand, within SGL_RS13535 at 2508.701 kb on - strand, within SGL_RS13535 at 2508.733 kb on + strand, within SGL_RS13535 at 2508.741 kb on - strand, within SGL_RS13535 at 2508.757 kb on - strand, within SGL_RS13535 at 2508.977 kb on + strand, within SGL_RS13535 at 2508.990 kb on + strand at 2509.158 kb on - strand at 2509.158 kb on - strand at 2509.377 kb on - strand, within SGL_RS13540 at 2509.633 kb on + strand at 2509.734 kb on - strand at 2509.921 kb on + strand, within SGL_RS13545
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,508,313 + -4.2 2,508,405 - -1.8 2,508,693 + SGL_RS13535 0.39 -3.2 2,508,701 - SGL_RS13535 0.40 -2.5 2,508,733 + SGL_RS13535 0.46 -1.8 2,508,741 - SGL_RS13535 0.47 -2.8 2,508,757 - SGL_RS13535 0.50 -1.5 2,508,977 + SGL_RS13535 0.90 -1.5 2,508,990 + -3.0 2,509,158 - -0.6 2,509,158 - +0.4 2,509,377 - SGL_RS13540 0.51 +0.0 2,509,633 + +0.2 2,509,734 - +0.3 2,509,921 + SGL_RS13545 0.51 -0.4
Or see this region's nucleotide sequence