Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13490 and SGL_RS13495 are separated by 103 nucleotides SGL_RS13495 and SGL_RS13500 are separated by 187 nucleotides
SGL_RS13490: SGL_RS13490 - aspartate aminotransferase, at 2,494,205 to 2,495,389
_RS13490
SGL_RS13495: SGL_RS13495 - AarF/ABC1/UbiB kinase family protein, at 2,495,493 to 2,497,538
_RS13495
SGL_RS13500: SGL_RS13500 - ATP-dependent DNA helicase RecG, at 2,497,726 to 2,500,221
_RS13500
Position (kb)
2495
2496
2497
2498 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 2494.757 kb on + strand, within SGL_RS13490 at 2495.050 kb on - strand, within SGL_RS13490 at 2495.050 kb on - strand, within SGL_RS13490 at 2495.050 kb on - strand, within SGL_RS13490 at 2495.056 kb on + strand, within SGL_RS13490 at 2495.350 kb on - strand at 2495.436 kb on + strand at 2495.436 kb on + strand at 2495.550 kb on - strand at 2495.667 kb on - strand at 2495.669 kb on + strand at 2495.677 kb on - strand at 2495.785 kb on + strand, within SGL_RS13495 at 2495.793 kb on - strand, within SGL_RS13495 at 2495.793 kb on - strand, within SGL_RS13495 at 2496.080 kb on + strand, within SGL_RS13495 at 2496.317 kb on + strand, within SGL_RS13495 at 2496.384 kb on - strand, within SGL_RS13495 at 2496.440 kb on - strand, within SGL_RS13495 at 2496.466 kb on - strand, within SGL_RS13495 at 2496.647 kb on + strand, within SGL_RS13495 at 2496.743 kb on - strand, within SGL_RS13495 at 2496.815 kb on - strand, within SGL_RS13495 at 2496.865 kb on + strand, within SGL_RS13495 at 2496.928 kb on - strand, within SGL_RS13495 at 2496.978 kb on - strand, within SGL_RS13495 at 2496.982 kb on + strand, within SGL_RS13495 at 2496.983 kb on + strand, within SGL_RS13495 at 2497.074 kb on + strand, within SGL_RS13495 at 2497.652 kb on - strand at 2497.695 kb on + strand at 2497.726 kb on + strand at 2497.980 kb on + strand, within SGL_RS13500 at 2498.089 kb on + strand, within SGL_RS13500 at 2498.117 kb on + strand, within SGL_RS13500 at 2498.304 kb on - strand, within SGL_RS13500 at 2498.331 kb on - strand, within SGL_RS13500
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,494,757 + SGL_RS13490 0.47 +1.2 2,495,050 - SGL_RS13490 0.71 +1.5 2,495,050 - SGL_RS13490 0.71 +1.1 2,495,050 - SGL_RS13490 0.71 +1.1 2,495,056 + SGL_RS13490 0.72 +0.8 2,495,350 - +1.1 2,495,436 + +0.6 2,495,436 + +0.3 2,495,550 - +1.4 2,495,667 - -6.2 2,495,669 + -5.3 2,495,677 - -6.1 2,495,785 + SGL_RS13495 0.14 -5.9 2,495,793 - SGL_RS13495 0.15 -5.1 2,495,793 - SGL_RS13495 0.15 -3.5 2,496,080 + SGL_RS13495 0.29 -4.1 2,496,317 + SGL_RS13495 0.40 -5.3 2,496,384 - SGL_RS13495 0.44 -6.6 2,496,440 - SGL_RS13495 0.46 -6.4 2,496,466 - SGL_RS13495 0.48 -6.6 2,496,647 + SGL_RS13495 0.56 -4.5 2,496,743 - SGL_RS13495 0.61 -4.3 2,496,815 - SGL_RS13495 0.65 -5.9 2,496,865 + SGL_RS13495 0.67 -5.6 2,496,928 - SGL_RS13495 0.70 -5.1 2,496,978 - SGL_RS13495 0.73 -4.8 2,496,982 + SGL_RS13495 0.73 -4.8 2,496,983 + SGL_RS13495 0.73 -3.6 2,497,074 + SGL_RS13495 0.77 -5.0 2,497,652 - -1.9 2,497,695 + +0.5 2,497,726 + +0.1 2,497,980 + SGL_RS13500 0.10 +0.7 2,498,089 + SGL_RS13500 0.15 -0.6 2,498,117 + SGL_RS13500 0.16 +2.3 2,498,304 - SGL_RS13500 0.23 +0.7 2,498,331 - SGL_RS13500 0.24 +1.2
Or see this region's nucleotide sequence