Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13185 and SGL_RS13190 overlap by 4 nucleotides SGL_RS13190 and SGL_RS13195 overlap by 47 nucleotides SGL_RS13195 and SGL_RS13200 are separated by 222 nucleotides
SGL_RS13185: SGL_RS13185 - AI-2E family transporter, at 2,424,371 to 2,425,390
_RS13185
SGL_RS13190: SGL_RS13190 - amino acid ABC transporter substrate-binding protein, at 2,425,387 to 2,426,343
_RS13190
SGL_RS13195: SGL_RS13195 - Ig-like domain-containing protein, at 2,426,297 to 2,427,787
_RS13195
SGL_RS13200: SGL_RS13200 - cytochrome b6, at 2,428,010 to 2,428,678
_RS13200
Position (kb)
2426
2427
2428 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 2425.313 kb on + strand at 2425.315 kb on - strand at 2425.368 kb on - strand at 2425.478 kb on + strand at 2425.478 kb on + strand at 2425.486 kb on - strand, within SGL_RS13190 at 2425.486 kb on - strand, within SGL_RS13190 at 2425.498 kb on - strand, within SGL_RS13190 at 2425.510 kb on + strand, within SGL_RS13190 at 2425.905 kb on - strand, within SGL_RS13190 at 2426.049 kb on + strand, within SGL_RS13190 at 2426.068 kb on - strand, within SGL_RS13190 at 2426.075 kb on + strand, within SGL_RS13190 at 2426.294 kb on + strand at 2426.321 kb on - strand at 2426.357 kb on + strand at 2426.636 kb on + strand, within SGL_RS13195 at 2426.818 kb on - strand, within SGL_RS13195 at 2426.990 kb on - strand, within SGL_RS13195 at 2427.113 kb on + strand, within SGL_RS13195 at 2427.147 kb on + strand, within SGL_RS13195 at 2427.241 kb on + strand, within SGL_RS13195 at 2427.389 kb on + strand, within SGL_RS13195 at 2427.396 kb on - strand, within SGL_RS13195 at 2427.480 kb on + strand, within SGL_RS13195 at 2427.480 kb on + strand, within SGL_RS13195 at 2427.695 kb on - strand at 2427.728 kb on - strand at 2427.735 kb on + strand at 2427.788 kb on + strand at 2427.788 kb on + strand at 2427.796 kb on - strand at 2427.917 kb on - strand at 2428.745 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,425,313 + +1.8 2,425,315 - +0.5 2,425,368 - +2.9 2,425,478 + -0.8 2,425,478 + -1.3 2,425,486 - SGL_RS13190 0.10 -0.1 2,425,486 - SGL_RS13190 0.10 -0.8 2,425,498 - SGL_RS13190 0.12 -0.5 2,425,510 + SGL_RS13190 0.13 +0.4 2,425,905 - SGL_RS13190 0.54 -1.3 2,426,049 + SGL_RS13190 0.69 -0.1 2,426,068 - SGL_RS13190 0.71 -1.9 2,426,075 + SGL_RS13190 0.72 -0.8 2,426,294 + -0.3 2,426,321 - -2.7 2,426,357 + -4.3 2,426,636 + SGL_RS13195 0.23 -1.1 2,426,818 - SGL_RS13195 0.35 -4.0 2,426,990 - SGL_RS13195 0.46 -2.8 2,427,113 + SGL_RS13195 0.55 -5.1 2,427,147 + SGL_RS13195 0.57 -2.8 2,427,241 + SGL_RS13195 0.63 -4.6 2,427,389 + SGL_RS13195 0.73 -4.0 2,427,396 - SGL_RS13195 0.74 -4.1 2,427,480 + SGL_RS13195 0.79 -3.2 2,427,480 + SGL_RS13195 0.79 -4.8 2,427,695 - -5.5 2,427,728 - -5.6 2,427,735 + -1.5 2,427,788 + +0.3 2,427,788 + +0.3 2,427,796 - +0.5 2,427,917 - -4.8 2,428,745 + +2.7
Or see this region's nucleotide sequence