Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12760 and SGL_RS12765 are separated by 88 nucleotides SGL_RS12765 and SGL_RS12770 overlap by 20 nucleotides SGL_RS12770 and SGL_RS12775 overlap by 73 nucleotides
SGL_RS12760: SGL_RS12760 - hypothetical protein, at 2,317,112 to 2,317,447
_RS12760
SGL_RS12765: SGL_RS12765 - hypothetical protein, at 2,317,536 to 2,318,030
_RS12765
SGL_RS12770: SGL_RS12770 - uroporphyrinogen-III C-methyltransferase, at 2,318,011 to 2,319,627
_RS12770
SGL_RS12775: SGL_RS12775 - hypothetical protein, at 2,319,555 to 2,324,636
_RS12775
Position (kb)
2318
2319
2320 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2317.032 kb on - strand at 2317.162 kb on - strand, within SGL_RS12760 at 2317.270 kb on - strand, within SGL_RS12760 at 2317.279 kb on + strand, within SGL_RS12760 at 2317.280 kb on - strand, within SGL_RS12760 at 2317.579 kb on - strand at 2317.609 kb on + strand, within SGL_RS12765 at 2317.628 kb on - strand, within SGL_RS12765 at 2317.724 kb on + strand, within SGL_RS12765 at 2317.769 kb on - strand, within SGL_RS12765 at 2317.824 kb on - strand, within SGL_RS12765 at 2317.833 kb on + strand, within SGL_RS12765 at 2317.833 kb on + strand, within SGL_RS12765 at 2317.936 kb on - strand, within SGL_RS12765 at 2319.071 kb on + strand, within SGL_RS12770 at 2319.079 kb on - strand, within SGL_RS12770 at 2319.090 kb on + strand, within SGL_RS12770 at 2319.135 kb on - strand, within SGL_RS12770 at 2319.331 kb on + strand, within SGL_RS12770 at 2319.346 kb on + strand, within SGL_RS12770 at 2319.544 kb on - strand at 2319.699 kb on + strand at 2319.699 kb on + strand at 2319.729 kb on - strand at 2319.776 kb on - strand at 2319.789 kb on + strand at 2319.798 kb on - strand at 2320.011 kb on + strand at 2320.019 kb on - strand at 2320.095 kb on + strand, within SGL_RS12775 at 2320.098 kb on - strand, within SGL_RS12775 at 2320.358 kb on - strand, within SGL_RS12775 at 2320.464 kb on + strand, within SGL_RS12775 at 2320.528 kb on + strand, within SGL_RS12775 at 2320.551 kb on - strand, within SGL_RS12775 at 2320.569 kb on - strand, within SGL_RS12775
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,317,032 - -6.8 2,317,162 - SGL_RS12760 0.15 -1.5 2,317,270 - SGL_RS12760 0.47 -1.2 2,317,279 + SGL_RS12760 0.50 -0.6 2,317,280 - SGL_RS12760 0.50 -0.3 2,317,579 - -0.4 2,317,609 + SGL_RS12765 0.15 -0.5 2,317,628 - SGL_RS12765 0.19 -0.6 2,317,724 + SGL_RS12765 0.38 -0.5 2,317,769 - SGL_RS12765 0.47 -1.5 2,317,824 - SGL_RS12765 0.58 -0.7 2,317,833 + SGL_RS12765 0.60 -0.6 2,317,833 + SGL_RS12765 0.60 -0.5 2,317,936 - SGL_RS12765 0.81 -0.6 2,319,071 + SGL_RS12770 0.66 -2.7 2,319,079 - SGL_RS12770 0.66 -4.6 2,319,090 + SGL_RS12770 0.67 -2.3 2,319,135 - SGL_RS12770 0.70 -3.6 2,319,331 + SGL_RS12770 0.82 -3.8 2,319,346 + SGL_RS12770 0.83 -4.1 2,319,544 - -0.8 2,319,699 + -0.4 2,319,699 + -0.8 2,319,729 - +0.2 2,319,776 - -0.7 2,319,789 + -0.3 2,319,798 - +0.3 2,320,011 + -0.8 2,320,019 - -0.8 2,320,095 + SGL_RS12775 0.11 +0.1 2,320,098 - SGL_RS12775 0.11 -0.7 2,320,358 - SGL_RS12775 0.16 -0.6 2,320,464 + SGL_RS12775 0.18 -0.9 2,320,528 + SGL_RS12775 0.19 +0.2 2,320,551 - SGL_RS12775 0.20 -2.5 2,320,569 - SGL_RS12775 0.20 +1.5
Or see this region's nucleotide sequence