Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12735 and SGL_RS12740 are separated by 61 nucleotides SGL_RS12740 and SGL_RS12745 are separated by 385 nucleotides SGL_RS12745 and SGL_RS12750 are separated by 168 nucleotides
SGL_RS12735: SGL_RS12735 - virginiamycin B hydrolase Vgb, at 2,309,413 to 2,310,471
_RS12735
SGL_RS12740: SGL_RS12740 - hypothetical protein, at 2,310,533 to 2,310,991
_RS12740
SGL_RS12745: SGL_RS12745 - glycine cleavage system aminomethyltransferase GcvT, at 2,311,377 to 2,312,495
_RS12745
SGL_RS12750: SGL_RS12750 - molecular chaperone DnaK, at 2,312,664 to 2,314,574
_RS12750
Position (kb)
2311
2312
2313 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2310.456 kb on - strand at 2310.497 kb on - strand at 2310.578 kb on - strand at 2310.590 kb on - strand, within SGL_RS12740 at 2310.724 kb on + strand, within SGL_RS12740 at 2310.780 kb on + strand, within SGL_RS12740 at 2310.835 kb on + strand, within SGL_RS12740 at 2310.900 kb on - strand, within SGL_RS12740 at 2310.902 kb on - strand, within SGL_RS12740 at 2310.950 kb on + strand at 2310.958 kb on - strand at 2310.958 kb on - strand at 2311.152 kb on - strand at 2311.276 kb on + strand at 2311.528 kb on - strand, within SGL_RS12745 at 2311.528 kb on - strand, within SGL_RS12745 at 2311.690 kb on - strand, within SGL_RS12745 at 2311.799 kb on - strand, within SGL_RS12745 at 2311.810 kb on - strand, within SGL_RS12745 at 2311.825 kb on - strand, within SGL_RS12745 at 2312.217 kb on + strand, within SGL_RS12745 at 2312.224 kb on + strand, within SGL_RS12745 at 2312.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,310,456 - +0.9 2,310,497 - +0.4 2,310,578 - -0.1 2,310,590 - SGL_RS12740 0.12 +0.4 2,310,724 + SGL_RS12740 0.42 +0.1 2,310,780 + SGL_RS12740 0.54 +0.5 2,310,835 + SGL_RS12740 0.66 +0.2 2,310,900 - SGL_RS12740 0.80 -1.0 2,310,902 - SGL_RS12740 0.80 +0.3 2,310,950 + -0.2 2,310,958 - -0.0 2,310,958 - -0.9 2,311,152 - -0.5 2,311,276 + +0.4 2,311,528 - SGL_RS12745 0.13 -3.3 2,311,528 - SGL_RS12745 0.13 -2.3 2,311,690 - SGL_RS12745 0.28 -4.2 2,311,799 - SGL_RS12745 0.38 -3.6 2,311,810 - SGL_RS12745 0.39 -3.2 2,311,825 - SGL_RS12745 0.40 -2.8 2,312,217 + SGL_RS12745 0.75 -3.4 2,312,224 + SGL_RS12745 0.76 -2.8 2,312,592 - +0.4
Or see this region's nucleotide sequence