Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12555 and SGL_RS12560 overlap by 8 nucleotides SGL_RS12560 and SGL_RS12565 are separated by 47 nucleotides SGL_RS12565 and SGL_RS12570 are separated by 90 nucleotides
SGL_RS12555: SGL_RS12555 - alpha-mannosidase, at 2,274,868 to 2,277,996
_RS12555
SGL_RS12560: SGL_RS12560 - ABC transporter permease, at 2,277,989 to 2,278,873
_RS12560
SGL_RS12565: SGL_RS12565 - MEKHLA domain-containing protein, at 2,278,921 to 2,279,406
_RS12565
SGL_RS12570: SGL_RS12570 - pentapeptide repeat-containing protein, at 2,279,497 to 2,280,006
_RS12570
Position (kb)
2277
2278
2279 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2
3
4 at 2277.200 kb on + strand, within SGL_RS12555 at 2277.208 kb on - strand, within SGL_RS12555 at 2278.254 kb on + strand, within SGL_RS12560 at 2278.267 kb on + strand, within SGL_RS12560 at 2278.310 kb on - strand, within SGL_RS12560 at 2278.372 kb on + strand, within SGL_RS12560 at 2278.382 kb on - strand, within SGL_RS12560 at 2278.734 kb on + strand, within SGL_RS12560 at 2278.996 kb on - strand, within SGL_RS12565 at 2279.287 kb on + strand, within SGL_RS12565 at 2279.872 kb on + strand, within SGL_RS12570
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,277,200 + SGL_RS12555 0.75 +4.0 2,277,208 - SGL_RS12555 0.75 +3.1 2,278,254 + SGL_RS12560 0.30 -4.9 2,278,267 + SGL_RS12560 0.31 -7.1 2,278,310 - SGL_RS12560 0.36 -5.9 2,278,372 + SGL_RS12560 0.43 -4.3 2,278,382 - SGL_RS12560 0.44 -6.9 2,278,734 + SGL_RS12560 0.84 -5.9 2,278,996 - SGL_RS12565 0.15 -0.2 2,279,287 + SGL_RS12565 0.75 +0.2 2,279,872 + SGL_RS12570 0.74 +0.1
Or see this region's nucleotide sequence