Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12535 and SGL_RS12540 are separated by 40 nucleotides SGL_RS12540 and SGL_RS12545 are separated by 460 nucleotides
SGL_RS12535: SGL_RS12535 - radical SAM protein, at 2,267,490 to 2,269,085
_RS12535
SGL_RS12540: SGL_RS12540 - RNA polymerase sigma factor, RpoD/SigA family, at 2,269,126 to 2,270,163
_RS12540
SGL_RS12545: SGL_RS12545 - GTPase Era, at 2,270,624 to 2,271,571
_RS12545
Position (kb)
2269
2270
2271 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 2268.172 kb on + strand, within SGL_RS12535 at 2268.277 kb on + strand, within SGL_RS12535 at 2268.299 kb on + strand, within SGL_RS12535 at 2268.448 kb on - strand, within SGL_RS12535 at 2268.450 kb on + strand, within SGL_RS12535 at 2268.458 kb on - strand, within SGL_RS12535 at 2268.458 kb on - strand, within SGL_RS12535 at 2268.619 kb on + strand, within SGL_RS12535 at 2268.852 kb on - strand, within SGL_RS12535 at 2268.917 kb on + strand, within SGL_RS12535 at 2268.998 kb on + strand at 2269.016 kb on - strand at 2269.016 kb on - strand at 2269.445 kb on - strand, within SGL_RS12540 at 2269.513 kb on - strand, within SGL_RS12540 at 2269.616 kb on + strand, within SGL_RS12540 at 2269.621 kb on + strand, within SGL_RS12540 at 2269.750 kb on - strand, within SGL_RS12540 at 2269.859 kb on - strand, within SGL_RS12540 at 2270.051 kb on - strand, within SGL_RS12540 at 2270.087 kb on + strand at 2270.197 kb on - strand at 2270.204 kb on + strand at 2270.204 kb on + strand at 2270.214 kb on + strand at 2270.317 kb on + strand at 2270.319 kb on - strand at 2270.463 kb on + strand at 2270.554 kb on - strand at 2270.710 kb on - strand at 2271.062 kb on - strand, within SGL_RS12545 at 2271.118 kb on - strand, within SGL_RS12545 at 2271.157 kb on - strand, within SGL_RS12545
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,268,172 + SGL_RS12535 0.43 +0.5 2,268,277 + SGL_RS12535 0.49 +0.5 2,268,299 + SGL_RS12535 0.51 +1.5 2,268,448 - SGL_RS12535 0.60 +0.5 2,268,450 + SGL_RS12535 0.60 -0.3 2,268,458 - SGL_RS12535 0.61 +0.8 2,268,458 - SGL_RS12535 0.61 +0.5 2,268,619 + SGL_RS12535 0.71 +0.9 2,268,852 - SGL_RS12535 0.85 +0.2 2,268,917 + SGL_RS12535 0.89 +0.9 2,268,998 + -0.4 2,269,016 - +0.3 2,269,016 - +0.3 2,269,445 - SGL_RS12540 0.31 -5.1 2,269,513 - SGL_RS12540 0.37 -7.0 2,269,616 + SGL_RS12540 0.47 -2.1 2,269,621 + SGL_RS12540 0.48 -2.9 2,269,750 - SGL_RS12540 0.60 -3.5 2,269,859 - SGL_RS12540 0.71 -7.6 2,270,051 - SGL_RS12540 0.89 -5.8 2,270,087 + -5.3 2,270,197 - -4.5 2,270,204 + -7.0 2,270,204 + -7.5 2,270,214 + -3.7 2,270,317 + -2.8 2,270,319 - -4.3 2,270,463 + -0.4 2,270,554 - +0.0 2,270,710 - -2.4 2,271,062 - SGL_RS12545 0.46 -3.2 2,271,118 - SGL_RS12545 0.52 +0.5 2,271,157 - SGL_RS12545 0.56 -4.4
Or see this region's nucleotide sequence