Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12410 and SGL_RS12415 are separated by 106 nucleotides SGL_RS12415 and SGL_RS12420 are separated by 97 nucleotides SGL_RS12420 and SGL_RS12425 are separated by 112 nucleotides
SGL_RS12410: SGL_RS12410 - M48 family metalloprotease, at 2,239,239 to 2,241,248
_RS12410
SGL_RS12415: SGL_RS12415 - collagen-like protein, at 2,241,355 to 2,242,788
_RS12415
SGL_RS12420: SGL_RS12420 - hypothetical protein, at 2,242,886 to 2,243,137
_RS12420
SGL_RS12425: SGL_RS12425 - TspO/MBR family protein, at 2,243,250 to 2,243,723
_RS12425
Position (kb)
2241
2242
2243 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2240.456 kb on + strand, within SGL_RS12410 at 2240.613 kb on + strand, within SGL_RS12410 at 2240.613 kb on + strand, within SGL_RS12410 at 2240.621 kb on - strand, within SGL_RS12410 at 2240.625 kb on + strand, within SGL_RS12410 at 2240.727 kb on + strand, within SGL_RS12410 at 2240.728 kb on + strand, within SGL_RS12410 at 2240.758 kb on - strand, within SGL_RS12410 at 2240.803 kb on - strand, within SGL_RS12410 at 2240.811 kb on + strand, within SGL_RS12410 at 2240.954 kb on - strand, within SGL_RS12410 at 2240.955 kb on + strand, within SGL_RS12410 at 2241.001 kb on - strand, within SGL_RS12410 at 2241.054 kb on + strand at 2241.064 kb on + strand at 2241.086 kb on - strand at 2241.096 kb on - strand at 2241.103 kb on + strand at 2241.338 kb on - strand at 2241.338 kb on - strand at 2241.411 kb on + strand at 2241.419 kb on - strand at 2241.442 kb on - strand at 2241.442 kb on - strand at 2241.442 kb on - strand at 2241.454 kb on + strand at 2241.605 kb on + strand, within SGL_RS12415 at 2241.642 kb on + strand, within SGL_RS12415 at 2241.674 kb on - strand, within SGL_RS12415 at 2241.684 kb on - strand, within SGL_RS12415 at 2241.870 kb on - strand, within SGL_RS12415 at 2241.894 kb on + strand, within SGL_RS12415 at 2241.902 kb on - strand, within SGL_RS12415 at 2242.022 kb on + strand, within SGL_RS12415 at 2242.061 kb on + strand, within SGL_RS12415 at 2242.071 kb on + strand, within SGL_RS12415 at 2242.102 kb on - strand, within SGL_RS12415 at 2242.102 kb on - strand, within SGL_RS12415 at 2242.370 kb on + strand, within SGL_RS12415 at 2242.537 kb on - strand, within SGL_RS12415 at 2242.567 kb on + strand, within SGL_RS12415 at 2242.575 kb on - strand, within SGL_RS12415 at 2242.580 kb on - strand, within SGL_RS12415 at 2243.122 kb on + strand at 2243.187 kb on - strand at 2243.405 kb on + strand, within SGL_RS12425 at 2243.420 kb on + strand, within SGL_RS12425 at 2243.420 kb on + strand, within SGL_RS12425 at 2243.428 kb on - strand, within SGL_RS12425 at 2243.428 kb on - strand, within SGL_RS12425 at 2243.451 kb on + strand, within SGL_RS12425 at 2243.459 kb on - strand, within SGL_RS12425 at 2243.459 kb on - strand, within SGL_RS12425 at 2243.459 kb on - strand, within SGL_RS12425 at 2243.459 kb on - strand, within SGL_RS12425 at 2243.493 kb on + strand, within SGL_RS12425 at 2243.505 kb on + strand, within SGL_RS12425 at 2243.553 kb on + strand, within SGL_RS12425 at 2243.664 kb on - strand, within SGL_RS12425
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,240,456 + SGL_RS12410 0.61 +0.1 2,240,613 + SGL_RS12410 0.68 -0.3 2,240,613 + SGL_RS12410 0.68 -0.2 2,240,621 - SGL_RS12410 0.69 -0.2 2,240,625 + SGL_RS12410 0.69 +0.6 2,240,727 + SGL_RS12410 0.74 -0.1 2,240,728 + SGL_RS12410 0.74 -0.2 2,240,758 - SGL_RS12410 0.76 +0.1 2,240,803 - SGL_RS12410 0.78 +0.1 2,240,811 + SGL_RS12410 0.78 -0.1 2,240,954 - SGL_RS12410 0.85 +0.2 2,240,955 + SGL_RS12410 0.85 +0.2 2,241,001 - SGL_RS12410 0.88 -0.2 2,241,054 + +0.3 2,241,064 + -0.5 2,241,086 - -0.3 2,241,096 - -0.5 2,241,103 + +0.1 2,241,338 - -2.0 2,241,338 - -2.8 2,241,411 + -0.8 2,241,419 - -1.5 2,241,442 - -1.6 2,241,442 - -2.3 2,241,442 - -1.6 2,241,454 + -1.9 2,241,605 + SGL_RS12415 0.17 -2.3 2,241,642 + SGL_RS12415 0.20 -1.1 2,241,674 - SGL_RS12415 0.22 -2.2 2,241,684 - SGL_RS12415 0.23 -2.3 2,241,870 - SGL_RS12415 0.36 -1.2 2,241,894 + SGL_RS12415 0.38 -2.1 2,241,902 - SGL_RS12415 0.38 -1.3 2,242,022 + SGL_RS12415 0.47 -1.4 2,242,061 + SGL_RS12415 0.49 -2.3 2,242,071 + SGL_RS12415 0.50 -1.9 2,242,102 - SGL_RS12415 0.52 -1.9 2,242,102 - SGL_RS12415 0.52 -1.3 2,242,370 + SGL_RS12415 0.71 -2.1 2,242,537 - SGL_RS12415 0.82 -1.0 2,242,567 + SGL_RS12415 0.85 -2.5 2,242,575 - SGL_RS12415 0.85 -1.4 2,242,580 - SGL_RS12415 0.85 -1.8 2,243,122 + -0.3 2,243,187 - +0.2 2,243,405 + SGL_RS12425 0.33 +0.1 2,243,420 + SGL_RS12425 0.36 -0.2 2,243,420 + SGL_RS12425 0.36 +0.4 2,243,428 - SGL_RS12425 0.38 +0.2 2,243,428 - SGL_RS12425 0.38 -0.2 2,243,451 + SGL_RS12425 0.42 +0.2 2,243,459 - SGL_RS12425 0.44 +0.5 2,243,459 - SGL_RS12425 0.44 -1.2 2,243,459 - SGL_RS12425 0.44 +0.3 2,243,459 - SGL_RS12425 0.44 -2.8 2,243,493 + SGL_RS12425 0.51 +0.3 2,243,505 + SGL_RS12425 0.54 -0.2 2,243,553 + SGL_RS12425 0.64 -0.2 2,243,664 - SGL_RS12425 0.87 -0.0
Or see this region's nucleotide sequence