Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11945 and SGL_RS11950 are separated by 3 nucleotides SGL_RS11950 and SGL_RS11955 are separated by 179 nucleotides SGL_RS11955 and SGL_RS11960 are separated by 105 nucleotides SGL_RS11960 and SGL_RS11965 overlap by 32 nucleotides SGL_RS11965 and SGL_RS11970 overlap by 8 nucleotides
SGL_RS11945: SGL_RS11945 - sirohydrochlorin chelatase, at 2,132,715 to 2,133,545
_RS11945
SGL_RS11950: SGL_RS11950 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 2,133,549 to 2,134,022
_RS11950
SGL_RS11955: SGL_RS11955 - aminomethyl-transferring glycine dehydrogenase, at 2,134,202 to 2,137,153
_RS11955
SGL_RS11960: SGL_RS11960 - hypothetical protein, at 2,137,259 to 2,137,738
_RS11960
SGL_RS11965: SGL_RS11965 - hypothetical protein, at 2,137,707 to 2,138,081
_RS11965
SGL_RS11970: SGL_RS11970 - HNH endonuclease, at 2,138,074 to 2,138,439
_RS11970
Position (kb)
2134
2135
2136
2137
2138 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2133.464 kb on - strand at 2133.490 kb on + strand at 2133.546 kb on + strand at 2133.554 kb on - strand at 2133.588 kb on + strand at 2133.596 kb on - strand at 2133.596 kb on - strand at 2133.692 kb on + strand, within SGL_RS11950 at 2133.786 kb on + strand, within SGL_RS11950 at 2133.864 kb on - strand, within SGL_RS11950 at 2133.872 kb on - strand, within SGL_RS11950 at 2133.884 kb on - strand, within SGL_RS11950 at 2133.886 kb on + strand, within SGL_RS11950 at 2133.931 kb on + strand, within SGL_RS11950 at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.094 kb on + strand at 2134.102 kb on - strand at 2134.102 kb on - strand at 2134.102 kb on - strand at 2134.244 kb on - strand at 2134.251 kb on + strand at 2134.781 kb on + strand, within SGL_RS11955 at 2134.839 kb on + strand, within SGL_RS11955 at 2135.015 kb on - strand, within SGL_RS11955 at 2135.091 kb on + strand, within SGL_RS11955 at 2135.098 kb on + strand, within SGL_RS11955 at 2135.567 kb on - strand, within SGL_RS11955 at 2135.983 kb on + strand, within SGL_RS11955 at 2136.018 kb on - strand, within SGL_RS11955 at 2136.077 kb on + strand, within SGL_RS11955 at 2136.230 kb on - strand, within SGL_RS11955 at 2136.262 kb on - strand, within SGL_RS11955 at 2136.331 kb on - strand, within SGL_RS11955 at 2136.421 kb on - strand, within SGL_RS11955 at 2136.494 kb on + strand, within SGL_RS11955 at 2136.550 kb on - strand, within SGL_RS11955 at 2136.813 kb on - strand, within SGL_RS11955 at 2136.813 kb on - strand, within SGL_RS11955 at 2136.975 kb on - strand at 2137.119 kb on + strand at 2137.292 kb on + strand at 2137.417 kb on + strand, within SGL_RS11960 at 2137.499 kb on - strand, within SGL_RS11960 at 2137.676 kb on + strand, within SGL_RS11960
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,133,464 - -3.5 2,133,490 + -2.6 2,133,546 + -0.0 2,133,554 - +0.2 2,133,588 + +0.0 2,133,596 - +0.7 2,133,596 - -0.0 2,133,692 + SGL_RS11950 0.30 +0.5 2,133,786 + SGL_RS11950 0.50 +0.7 2,133,864 - SGL_RS11950 0.66 +0.0 2,133,872 - SGL_RS11950 0.68 -0.2 2,133,884 - SGL_RS11950 0.71 +0.3 2,133,886 + SGL_RS11950 0.71 +0.3 2,133,931 + SGL_RS11950 0.81 -0.1 2,134,094 + +0.1 2,134,094 + -0.1 2,134,094 + +0.7 2,134,094 + -0.6 2,134,094 + +0.1 2,134,094 + +0.0 2,134,094 + +0.1 2,134,102 - +0.1 2,134,102 - +0.4 2,134,102 - -0.5 2,134,244 - -3.0 2,134,251 + -3.1 2,134,781 + SGL_RS11955 0.20 -4.0 2,134,839 + SGL_RS11955 0.22 -0.6 2,135,015 - SGL_RS11955 0.28 -3.3 2,135,091 + SGL_RS11955 0.30 -3.3 2,135,098 + SGL_RS11955 0.30 -1.6 2,135,567 - SGL_RS11955 0.46 -3.5 2,135,983 + SGL_RS11955 0.60 -2.4 2,136,018 - SGL_RS11955 0.62 -3.0 2,136,077 + SGL_RS11955 0.64 -2.1 2,136,230 - SGL_RS11955 0.69 -2.7 2,136,262 - SGL_RS11955 0.70 -1.6 2,136,331 - SGL_RS11955 0.72 -2.1 2,136,421 - SGL_RS11955 0.75 -3.9 2,136,494 + SGL_RS11955 0.78 -1.1 2,136,550 - SGL_RS11955 0.80 -3.4 2,136,813 - SGL_RS11955 0.88 -2.6 2,136,813 - SGL_RS11955 0.88 -2.7 2,136,975 - -3.0 2,137,119 + +0.7 2,137,292 + -0.4 2,137,417 + SGL_RS11960 0.33 +0.0 2,137,499 - SGL_RS11960 0.50 -1.6 2,137,676 + SGL_RS11960 0.87 +0.0
Or see this region's nucleotide sequence