Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11755 and SGL_RS11760 are separated by 2 nucleotides SGL_RS11760 and SGL_RS11765 are separated by 80 nucleotides SGL_RS11765 and SGL_RS11770 are separated by 221 nucleotides SGL_RS11770 and SGL_RS11775 are separated by 47 nucleotides
SGL_RS11755: SGL_RS11755 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,088,680 to 2,090,023
_RS11755
SGL_RS11760: SGL_RS11760 - radical SAM protein, at 2,090,026 to 2,091,630
_RS11760
SGL_RS11765: SGL_RS11765 - hypothetical protein, at 2,091,711 to 2,092,178
_RS11765
SGL_RS11770: SGL_RS11770 - tRNA-Asn, at 2,092,400 to 2,092,471
_RS11770
SGL_RS11775: SGL_RS11775 - isoaspartyl peptidase/L-asparaginase, at 2,092,519 to 2,093,508
_RS11775
Position (kb)
2090
2091
2092 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2090.141 kb on - strand at 2090.261 kb on + strand, within SGL_RS11760 at 2090.369 kb on + strand, within SGL_RS11760 at 2090.370 kb on + strand, within SGL_RS11760 at 2090.389 kb on + strand, within SGL_RS11760 at 2090.390 kb on + strand, within SGL_RS11760 at 2090.547 kb on - strand, within SGL_RS11760 at 2090.568 kb on - strand, within SGL_RS11760 at 2090.652 kb on + strand, within SGL_RS11760 at 2090.708 kb on - strand, within SGL_RS11760 at 2090.715 kb on + strand, within SGL_RS11760 at 2090.796 kb on - strand, within SGL_RS11760 at 2090.840 kb on - strand, within SGL_RS11760 at 2091.494 kb on - strand at 2091.495 kb on - strand at 2091.601 kb on - strand at 2091.621 kb on - strand at 2091.763 kb on + strand, within SGL_RS11765 at 2091.818 kb on - strand, within SGL_RS11765 at 2091.863 kb on - strand, within SGL_RS11765 at 2091.980 kb on + strand, within SGL_RS11765 at 2092.005 kb on + strand, within SGL_RS11765 at 2092.013 kb on - strand, within SGL_RS11765 at 2092.086 kb on + strand, within SGL_RS11765 at 2092.263 kb on + strand at 2092.307 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,090,141 - -3.6 2,090,261 + SGL_RS11760 0.15 -5.6 2,090,369 + SGL_RS11760 0.21 -5.0 2,090,370 + SGL_RS11760 0.21 -5.7 2,090,389 + SGL_RS11760 0.23 -5.7 2,090,390 + SGL_RS11760 0.23 -5.5 2,090,547 - SGL_RS11760 0.32 -6.5 2,090,568 - SGL_RS11760 0.34 -6.2 2,090,652 + SGL_RS11760 0.39 -5.3 2,090,708 - SGL_RS11760 0.42 -6.0 2,090,715 + SGL_RS11760 0.43 -3.2 2,090,796 - SGL_RS11760 0.48 -6.0 2,090,840 - SGL_RS11760 0.51 -5.9 2,091,494 - -5.7 2,091,495 - -5.8 2,091,601 - -6.2 2,091,621 - -5.0 2,091,763 + SGL_RS11765 0.11 -3.6 2,091,818 - SGL_RS11765 0.23 -0.5 2,091,863 - SGL_RS11765 0.32 -0.0 2,091,980 + SGL_RS11765 0.57 -5.4 2,092,005 + SGL_RS11765 0.63 -6.7 2,092,013 - SGL_RS11765 0.65 -1.7 2,092,086 + SGL_RS11765 0.80 -3.8 2,092,263 + +0.0 2,092,307 + +0.1
Or see this region's nucleotide sequence