Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11315 and SGL_RS11320 are separated by 46 nucleotides SGL_RS11320 and SGL_RS11325 overlap by 4 nucleotides SGL_RS11325 and SGL_RS11330 are separated by 160 nucleotides
SGL_RS11315: SGL_RS11315 - Mur ligase family protein, at 2,000,815 to 2,002,194
_RS11315
SGL_RS11320: SGL_RS11320 - precorrin-8X methylmutase, at 2,002,241 to 2,002,894
_RS11320
SGL_RS11325: SGL_RS11325 - pitrilysin family protein, at 2,002,891 to 2,004,465
_RS11325
SGL_RS11330: SGL_RS11330 - 9,9'-di-cis-zeta-carotene desaturase, at 2,004,626 to 2,006,095
_RS11330
Position (kb)
2002
2003
2004
2005 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2002.208 kb on + strand at 2002.209 kb on + strand at 2002.603 kb on - strand, within SGL_RS11320 at 2002.649 kb on + strand, within SGL_RS11320 at 2002.783 kb on + strand, within SGL_RS11320 at 2002.851 kb on - strand at 2003.216 kb on - strand, within SGL_RS11325 at 2003.501 kb on + strand, within SGL_RS11325 at 2003.525 kb on + strand, within SGL_RS11325 at 2003.787 kb on - strand, within SGL_RS11325 at 2003.837 kb on + strand, within SGL_RS11325 at 2003.951 kb on - strand, within SGL_RS11325 at 2003.971 kb on - strand, within SGL_RS11325 at 2003.971 kb on - strand, within SGL_RS11325 at 2004.072 kb on - strand, within SGL_RS11325 at 2004.196 kb on - strand, within SGL_RS11325 at 2004.220 kb on - strand, within SGL_RS11325 at 2004.699 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 2,002,208 + -0.0 2,002,209 + -0.2 2,002,603 - SGL_RS11320 0.55 -2.6 2,002,649 + SGL_RS11320 0.62 -2.9 2,002,783 + SGL_RS11320 0.83 -2.4 2,002,851 - -2.2 2,003,216 - SGL_RS11325 0.21 -2.4 2,003,501 + SGL_RS11325 0.39 -4.4 2,003,525 + SGL_RS11325 0.40 -4.0 2,003,787 - SGL_RS11325 0.57 -5.5 2,003,837 + SGL_RS11325 0.60 -4.0 2,003,951 - SGL_RS11325 0.67 -3.6 2,003,971 - SGL_RS11325 0.69 -3.9 2,003,971 - SGL_RS11325 0.69 -1.4 2,004,072 - SGL_RS11325 0.75 -5.8 2,004,196 - SGL_RS11325 0.83 -2.9 2,004,220 - SGL_RS11325 0.84 -2.6 2,004,699 + -1.9
Or see this region's nucleotide sequence