Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10865 and SGL_RS10870 are separated by 100 nucleotides SGL_RS10870 and SGL_RS10875 are separated by 192 nucleotides
SGL_RS10865: SGL_RS10865 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB, at 1,902,437 to 1,903,924
_RS10865
SGL_RS10870: SGL_RS10870 - penicillin-binding protein 1A, at 1,904,025 to 1,905,977
_RS10870
SGL_RS10875: SGL_RS10875 - NAD(P)H-hydrate dehydratase, at 1,906,170 to 1,907,744
_RS10875
Position (kb)
1904
1905
1906 Strain fitness (log2 ratio)
-2
-1
0
1 at 1903.994 kb on + strand at 1904.002 kb on - strand at 1904.092 kb on + strand at 1904.285 kb on + strand, within SGL_RS10870 at 1904.293 kb on - strand, within SGL_RS10870 at 1904.293 kb on - strand, within SGL_RS10870 at 1904.425 kb on - strand, within SGL_RS10870 at 1904.450 kb on - strand, within SGL_RS10870 at 1904.481 kb on + strand, within SGL_RS10870 at 1904.483 kb on - strand, within SGL_RS10870 at 1904.488 kb on + strand, within SGL_RS10870 at 1904.496 kb on - strand, within SGL_RS10870 at 1904.496 kb on - strand, within SGL_RS10870 at 1904.496 kb on - strand, within SGL_RS10870 at 1904.549 kb on - strand, within SGL_RS10870 at 1904.610 kb on - strand, within SGL_RS10870 at 1904.708 kb on - strand, within SGL_RS10870 at 1904.772 kb on + strand, within SGL_RS10870 at 1904.925 kb on + strand, within SGL_RS10870 at 1905.155 kb on - strand, within SGL_RS10870 at 1905.205 kb on - strand, within SGL_RS10870 at 1905.258 kb on + strand, within SGL_RS10870 at 1905.584 kb on - strand, within SGL_RS10870 at 1905.726 kb on - strand, within SGL_RS10870 at 1905.857 kb on + strand at 1905.858 kb on + strand at 1905.931 kb on + strand at 1905.939 kb on - strand at 1905.953 kb on + strand at 1905.987 kb on - strand at 1905.987 kb on - strand at 1906.082 kb on + strand at 1906.118 kb on + strand at 1906.126 kb on - strand at 1906.242 kb on + strand at 1906.504 kb on + strand, within SGL_RS10875 at 1906.733 kb on + strand, within SGL_RS10875 at 1906.736 kb on - strand, within SGL_RS10875 at 1906.748 kb on - strand, within SGL_RS10875 at 1906.766 kb on - strand, within SGL_RS10875 at 1906.800 kb on + strand, within SGL_RS10875 at 1906.858 kb on - strand, within SGL_RS10875 at 1906.870 kb on + strand, within SGL_RS10875 at 1906.870 kb on + strand, within SGL_RS10875 at 1906.933 kb on - strand, within SGL_RS10875
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,903,994 + -0.0 1,904,002 - -0.1 1,904,092 + -1.6 1,904,285 + SGL_RS10870 0.13 -1.9 1,904,293 - SGL_RS10870 0.14 -2.4 1,904,293 - SGL_RS10870 0.14 -1.2 1,904,425 - SGL_RS10870 0.20 -1.5 1,904,450 - SGL_RS10870 0.22 -1.8 1,904,481 + SGL_RS10870 0.23 -2.5 1,904,483 - SGL_RS10870 0.23 -1.7 1,904,488 + SGL_RS10870 0.24 -0.9 1,904,496 - SGL_RS10870 0.24 -1.5 1,904,496 - SGL_RS10870 0.24 -1.7 1,904,496 - SGL_RS10870 0.24 -1.6 1,904,549 - SGL_RS10870 0.27 -1.7 1,904,610 - SGL_RS10870 0.30 -1.5 1,904,708 - SGL_RS10870 0.35 -1.8 1,904,772 + SGL_RS10870 0.38 -0.6 1,904,925 + SGL_RS10870 0.46 -1.3 1,905,155 - SGL_RS10870 0.58 -2.4 1,905,205 - SGL_RS10870 0.60 -1.1 1,905,258 + SGL_RS10870 0.63 -1.8 1,905,584 - SGL_RS10870 0.80 -1.9 1,905,726 - SGL_RS10870 0.87 -1.6 1,905,857 + -1.6 1,905,858 + -1.9 1,905,931 + -1.2 1,905,939 - -1.6 1,905,953 + -0.9 1,905,987 - -1.2 1,905,987 - -0.2 1,906,082 + +0.2 1,906,118 + +0.1 1,906,126 - +0.3 1,906,242 + +0.1 1,906,504 + SGL_RS10875 0.21 -0.1 1,906,733 + SGL_RS10875 0.36 +0.0 1,906,736 - SGL_RS10875 0.36 +0.2 1,906,748 - SGL_RS10875 0.37 -0.5 1,906,766 - SGL_RS10875 0.38 -0.4 1,906,800 + SGL_RS10875 0.40 -0.6 1,906,858 - SGL_RS10875 0.44 +0.2 1,906,870 + SGL_RS10875 0.44 +0.2 1,906,870 + SGL_RS10875 0.44 -0.0 1,906,933 - SGL_RS10875 0.48 +0.5
Or see this region's nucleotide sequence