Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10465 and SGL_RS10470 are separated by 235 nucleotides SGL_RS10470 and SGL_RS10475 are separated by 130 nucleotides SGL_RS10475 and SGL_RS10480 are separated by 281 nucleotides SGL_RS10480 and SGL_RS10485 are separated by 220 nucleotides SGL_RS10485 and SGL_RS10490 are separated by 131 nucleotides
SGL_RS10465: SGL_RS10465 - universal stress protein, at 1,822,666 to 1,823,130
_RS10465
SGL_RS10470: SGL_RS10470 - tRNA-Arg, at 1,823,366 to 1,823,439
_RS10470
SGL_RS10475: SGL_RS10475 - cytochrome b6-f complex subunit PetG, at 1,823,570 to 1,823,686
_RS10475
SGL_RS10480: SGL_RS10480 - metallophosphoesterase family protein, at 1,823,968 to 1,824,732
_RS10480
SGL_RS10485: SGL_RS10485 - ComEA family DNA-binding protein, at 1,824,953 to 1,825,468
_RS10485
SGL_RS10490: SGL_RS10490 - precorrin-8X methylmutase, at 1,825,600 to 1,826,226
_RS10490
Position (kb)
1823
1824
1825 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1823.023 kb on - strand, within SGL_RS10465 at 1823.097 kb on + strand at 1823.265 kb on - strand at 1824.016 kb on + strand at 1824.036 kb on + strand at 1824.079 kb on + strand, within SGL_RS10480 at 1824.188 kb on + strand, within SGL_RS10480 at 1824.214 kb on + strand, within SGL_RS10480 at 1824.239 kb on - strand, within SGL_RS10480 at 1824.349 kb on + strand, within SGL_RS10480 at 1824.349 kb on + strand, within SGL_RS10480 at 1824.352 kb on - strand, within SGL_RS10480 at 1824.357 kb on - strand, within SGL_RS10480 at 1824.380 kb on + strand, within SGL_RS10480 at 1824.383 kb on - strand, within SGL_RS10480 at 1824.415 kb on + strand, within SGL_RS10480 at 1824.517 kb on + strand, within SGL_RS10480 at 1824.627 kb on + strand, within SGL_RS10480 at 1824.772 kb on + strand at 1824.857 kb on + strand at 1824.868 kb on + strand at 1824.876 kb on - strand at 1824.902 kb on + strand at 1825.418 kb on - strand at 1825.665 kb on + strand, within SGL_RS10490 at 1825.671 kb on - strand, within SGL_RS10490 at 1825.678 kb on - strand, within SGL_RS10490 at 1825.680 kb on + strand, within SGL_RS10490 at 1825.680 kb on + strand, within SGL_RS10490 at 1825.688 kb on - strand, within SGL_RS10490
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,823,023 - SGL_RS10465 0.77 -0.8 1,823,097 + +0.2 1,823,265 - -0.3 1,824,016 + +1.0 1,824,036 + -3.2 1,824,079 + SGL_RS10480 0.15 -4.8 1,824,188 + SGL_RS10480 0.29 -7.1 1,824,214 + SGL_RS10480 0.32 -3.8 1,824,239 - SGL_RS10480 0.35 -1.9 1,824,349 + SGL_RS10480 0.50 -5.3 1,824,349 + SGL_RS10480 0.50 -2.4 1,824,352 - SGL_RS10480 0.50 -6.5 1,824,357 - SGL_RS10480 0.51 -4.4 1,824,380 + SGL_RS10480 0.54 -3.4 1,824,383 - SGL_RS10480 0.54 -3.4 1,824,415 + SGL_RS10480 0.58 -4.4 1,824,517 + SGL_RS10480 0.72 -3.3 1,824,627 + SGL_RS10480 0.86 -4.2 1,824,772 + -0.0 1,824,857 + +0.9 1,824,868 + -0.8 1,824,876 - +0.1 1,824,902 + +0.2 1,825,418 - -3.2 1,825,665 + SGL_RS10490 0.10 +1.3 1,825,671 - SGL_RS10490 0.11 +0.4 1,825,678 - SGL_RS10490 0.12 +1.0 1,825,680 + SGL_RS10490 0.13 +0.1 1,825,680 + SGL_RS10490 0.13 -1.3 1,825,688 - SGL_RS10490 0.14 -0.2
Or see this region's nucleotide sequence