Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10135 and SGL_RS10140 are separated by 252 nucleotides SGL_RS10140 and SGL_RS10145 are separated by 170 nucleotides SGL_RS10145 and SGL_RS10150 are separated by 243 nucleotides
SGL_RS10135: SGL_RS10135 - hypothetical protein, at 1,747,520 to 1,748,893
_RS10135
SGL_RS10140: SGL_RS10140 - UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase, at 1,749,146 to 1,750,510
_RS10140
SGL_RS10145: SGL_RS10145 - cytochrome c, at 1,750,681 to 1,751,067
_RS10145
SGL_RS10150: SGL_RS10150 - 50S ribosomal protein L9, at 1,751,311 to 1,751,769
_RS10150
Position (kb) 1749
1750
1751 Strain fitness (log2 ratio) -1
0
1 at 1748.156 kb on - strand, within SGL_RS10135 at 1748.311 kb on + strand, within SGL_RS10135 at 1748.311 kb on + strand, within SGL_RS10135 at 1748.316 kb on + strand, within SGL_RS10135 at 1748.326 kb on + strand, within SGL_RS10135 at 1748.334 kb on - strand, within SGL_RS10135 at 1748.334 kb on - strand, within SGL_RS10135 at 1748.372 kb on + strand, within SGL_RS10135 at 1748.882 kb on + strand at 1748.883 kb on + strand at 1749.104 kb on - strand at 1750.527 kb on - strand at 1750.548 kb on - strand at 1750.622 kb on + strand at 1750.829 kb on + strand, within SGL_RS10145 at 1750.889 kb on + strand, within SGL_RS10145 at 1751.070 kb on - strand at 1751.070 kb on - strand at 1751.175 kb on - strand at 1751.408 kb on + strand, within SGL_RS10150 at 1751.478 kb on - strand, within SGL_RS10150
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,748,156 - SGL_RS10135 0.46 +0.2 1,748,311 + SGL_RS10135 0.58 -0.1 1,748,311 + SGL_RS10135 0.58 -0.1 1,748,316 + SGL_RS10135 0.58 +0.1 1,748,326 + SGL_RS10135 0.59 -0.1 1,748,334 - SGL_RS10135 0.59 +0.3 1,748,334 - SGL_RS10135 0.59 +0.2 1,748,372 + SGL_RS10135 0.62 +0.3 1,748,882 + -1.1 1,748,883 + -0.1 1,749,104 - -0.5 1,750,527 - +0.3 1,750,548 - -0.2 1,750,622 + +0.1 1,750,829 + SGL_RS10145 0.38 -0.0 1,750,889 + SGL_RS10145 0.54 -0.6 1,751,070 - -0.0 1,751,070 - +0.4 1,751,175 - -0.6 1,751,408 + SGL_RS10150 0.21 -0.2 1,751,478 - SGL_RS10150 0.36 -0.1
Or see this region's nucleotide sequence