Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09835 and SGL_RS09840 overlap by 1 nucleotides SGL_RS09840 and SGL_RS09845 are separated by 67 nucleotides SGL_RS09845 and SGL_RS09850 are separated by 7 nucleotides
SGL_RS09835: SGL_RS09835 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 1,691,493 to 1,692,332
_RS09835
SGL_RS09840: SGL_RS09840 - hypothetical protein, at 1,692,332 to 1,692,559
_RS09840
SGL_RS09845: SGL_RS09845 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 1,692,627 to 1,693,538
_RS09845
SGL_RS09850: SGL_RS09850 - Gfo/Idh/MocA family oxidoreductase, at 1,693,546 to 1,694,661
_RS09850
Position (kb)
1692
1693
1694 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1691.650 kb on - strand, within SGL_RS09835 at 1691.793 kb on - strand, within SGL_RS09835 at 1691.886 kb on - strand, within SGL_RS09835 at 1691.963 kb on + strand, within SGL_RS09835 at 1691.971 kb on - strand, within SGL_RS09835 at 1691.985 kb on + strand, within SGL_RS09835 at 1692.025 kb on - strand, within SGL_RS09835 at 1692.051 kb on + strand, within SGL_RS09835 at 1692.059 kb on - strand, within SGL_RS09835 at 1692.140 kb on + strand, within SGL_RS09835 at 1692.140 kb on + strand, within SGL_RS09835 at 1692.373 kb on + strand, within SGL_RS09840 at 1692.373 kb on + strand, within SGL_RS09840 at 1692.413 kb on + strand, within SGL_RS09840 at 1692.464 kb on + strand, within SGL_RS09840 at 1692.472 kb on - strand, within SGL_RS09840 at 1692.551 kb on - strand at 1692.780 kb on - strand, within SGL_RS09845 at 1692.842 kb on + strand, within SGL_RS09845 at 1692.844 kb on + strand, within SGL_RS09845 at 1692.852 kb on - strand, within SGL_RS09845 at 1692.865 kb on + strand, within SGL_RS09845 at 1692.865 kb on + strand, within SGL_RS09845 at 1692.873 kb on - strand, within SGL_RS09845 at 1693.077 kb on - strand, within SGL_RS09845 at 1693.149 kb on + strand, within SGL_RS09845 at 1693.185 kb on + strand, within SGL_RS09845 at 1693.194 kb on - strand, within SGL_RS09845 at 1693.230 kb on + strand, within SGL_RS09845 at 1693.298 kb on - strand, within SGL_RS09845 at 1693.578 kb on + strand at 1693.583 kb on + strand at 1693.684 kb on + strand, within SGL_RS09850 at 1693.704 kb on - strand, within SGL_RS09850 at 1693.825 kb on + strand, within SGL_RS09850 at 1693.971 kb on - strand, within SGL_RS09850 at 1693.971 kb on - strand, within SGL_RS09850 at 1694.015 kb on + strand, within SGL_RS09850 at 1694.015 kb on + strand, within SGL_RS09850 at 1694.023 kb on - strand, within SGL_RS09850 at 1694.023 kb on - strand, within SGL_RS09850 at 1694.062 kb on - strand, within SGL_RS09850 at 1694.067 kb on - strand, within SGL_RS09850 at 1694.098 kb on + strand, within SGL_RS09850 at 1694.105 kb on - strand, within SGL_RS09850 at 1694.158 kb on - strand, within SGL_RS09850 at 1694.209 kb on - strand, within SGL_RS09850 at 1694.267 kb on - strand, within SGL_RS09850 at 1694.312 kb on - strand, within SGL_RS09850 at 1694.331 kb on + strand, within SGL_RS09850 at 1694.524 kb on + strand, within SGL_RS09850 at 1694.524 kb on + strand, within SGL_RS09850 at 1694.532 kb on - strand, within SGL_RS09850
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,691,650 - SGL_RS09835 0.19 -0.1 1,691,793 - SGL_RS09835 0.36 -0.5 1,691,886 - SGL_RS09835 0.47 +0.2 1,691,963 + SGL_RS09835 0.56 -0.5 1,691,971 - SGL_RS09835 0.57 +0.4 1,691,985 + SGL_RS09835 0.59 +0.5 1,692,025 - SGL_RS09835 0.63 -0.3 1,692,051 + SGL_RS09835 0.66 -0.4 1,692,059 - SGL_RS09835 0.67 -0.2 1,692,140 + SGL_RS09835 0.77 +0.1 1,692,140 + SGL_RS09835 0.77 -0.4 1,692,373 + SGL_RS09840 0.18 +0.1 1,692,373 + SGL_RS09840 0.18 -0.6 1,692,413 + SGL_RS09840 0.36 -1.5 1,692,464 + SGL_RS09840 0.58 -0.1 1,692,472 - SGL_RS09840 0.61 +0.4 1,692,551 - -0.4 1,692,780 - SGL_RS09845 0.17 -3.7 1,692,842 + SGL_RS09845 0.24 -1.9 1,692,844 + SGL_RS09845 0.24 -4.1 1,692,852 - SGL_RS09845 0.25 -4.7 1,692,865 + SGL_RS09845 0.26 -4.3 1,692,865 + SGL_RS09845 0.26 -2.2 1,692,873 - SGL_RS09845 0.27 -2.7 1,693,077 - SGL_RS09845 0.49 -2.8 1,693,149 + SGL_RS09845 0.57 -2.6 1,693,185 + SGL_RS09845 0.61 -4.0 1,693,194 - SGL_RS09845 0.62 -2.2 1,693,230 + SGL_RS09845 0.66 -4.2 1,693,298 - SGL_RS09845 0.74 -4.3 1,693,578 + -0.3 1,693,583 + -0.7 1,693,684 + SGL_RS09850 0.12 -1.0 1,693,704 - SGL_RS09850 0.14 -0.4 1,693,825 + SGL_RS09850 0.25 -0.1 1,693,971 - SGL_RS09850 0.38 -1.6 1,693,971 - SGL_RS09850 0.38 -0.3 1,694,015 + SGL_RS09850 0.42 -0.1 1,694,015 + SGL_RS09850 0.42 -0.7 1,694,023 - SGL_RS09850 0.43 -0.0 1,694,023 - SGL_RS09850 0.43 -0.3 1,694,062 - SGL_RS09850 0.46 -0.8 1,694,067 - SGL_RS09850 0.47 -0.3 1,694,098 + SGL_RS09850 0.49 -1.3 1,694,105 - SGL_RS09850 0.50 -0.7 1,694,158 - SGL_RS09850 0.55 -0.3 1,694,209 - SGL_RS09850 0.59 -0.2 1,694,267 - SGL_RS09850 0.65 -0.0 1,694,312 - SGL_RS09850 0.69 -1.6 1,694,331 + SGL_RS09850 0.70 -0.4 1,694,524 + SGL_RS09850 0.88 -1.8 1,694,524 + SGL_RS09850 0.88 -1.2 1,694,532 - SGL_RS09850 0.88 -1.2
Or see this region's nucleotide sequence