Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09375 and SGL_RS09380 are separated by 218 nucleotides SGL_RS09380 and SGL_RS09385 are separated by 20 nucleotides SGL_RS09385 and SGL_RS09390 are separated by 25 nucleotides SGL_RS09390 and SGL_RS09395 are separated by 109 nucleotides
SGL_RS09375: SGL_RS09375 - 3-isopropylmalate dehydrogenase, at 1,608,232 to 1,609,320
_RS09375
SGL_RS09380: SGL_RS09380 - hypothetical protein, at 1,609,539 to 1,609,805
_RS09380
SGL_RS09385: SGL_RS09385 - 2-carboxy-1,4-naphthoquinone phytyltransferase, at 1,609,826 to 1,610,749
_RS09385
SGL_RS09390: SGL_RS09390 - pentapeptide repeat-containing protein, at 1,610,775 to 1,611,464
_RS09390
SGL_RS09395: SGL_RS09395 - aldo/keto reductase, at 1,611,574 to 1,612,629
_RS09395
Position (kb) 1609
1610
1611 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1609.415 kb on + strand at 1609.456 kb on - strand at 1609.508 kb on + strand at 1609.574 kb on + strand, within SGL_RS09380 at 1609.583 kb on - strand, within SGL_RS09380 at 1609.654 kb on - strand, within SGL_RS09380 at 1609.666 kb on + strand, within SGL_RS09380 at 1609.750 kb on - strand, within SGL_RS09380 at 1609.775 kb on - strand, within SGL_RS09380 at 1610.241 kb on - strand, within SGL_RS09385 at 1610.271 kb on + strand, within SGL_RS09385 at 1610.434 kb on + strand, within SGL_RS09385 at 1610.441 kb on - strand, within SGL_RS09385 at 1610.602 kb on + strand, within SGL_RS09385 at 1610.610 kb on - strand, within SGL_RS09385 at 1610.738 kb on + strand at 1610.804 kb on - strand at 1610.881 kb on + strand, within SGL_RS09390 at 1610.889 kb on - strand, within SGL_RS09390 at 1610.889 kb on - strand, within SGL_RS09390 at 1610.916 kb on + strand, within SGL_RS09390 at 1610.918 kb on - strand, within SGL_RS09390 at 1610.919 kb on - strand, within SGL_RS09390 at 1611.029 kb on - strand, within SGL_RS09390 at 1611.046 kb on + strand, within SGL_RS09390 at 1611.075 kb on - strand, within SGL_RS09390 at 1611.174 kb on - strand, within SGL_RS09390 at 1611.182 kb on + strand, within SGL_RS09390 at 1611.189 kb on - strand, within SGL_RS09390 at 1611.198 kb on + strand, within SGL_RS09390 at 1611.203 kb on + strand, within SGL_RS09390 at 1611.224 kb on - strand, within SGL_RS09390 at 1611.321 kb on + strand, within SGL_RS09390 at 1611.421 kb on + strand at 1611.748 kb on + strand, within SGL_RS09395
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,609,415 + +0.2 1,609,456 - +1.0 1,609,508 + +1.7 1,609,574 + SGL_RS09380 0.13 +1.1 1,609,583 - SGL_RS09380 0.16 +3.4 1,609,654 - SGL_RS09380 0.43 +4.6 1,609,666 + SGL_RS09380 0.48 +2.2 1,609,750 - SGL_RS09380 0.79 +2.3 1,609,775 - SGL_RS09380 0.88 +3.5 1,610,241 - SGL_RS09385 0.45 -2.2 1,610,271 + SGL_RS09385 0.48 -2.2 1,610,434 + SGL_RS09385 0.66 -2.6 1,610,441 - SGL_RS09385 0.67 -2.8 1,610,602 + SGL_RS09385 0.84 -1.2 1,610,610 - SGL_RS09385 0.85 -1.9 1,610,738 + -1.6 1,610,804 - +0.3 1,610,881 + SGL_RS09390 0.15 -0.2 1,610,889 - SGL_RS09390 0.17 -0.3 1,610,889 - SGL_RS09390 0.17 -0.4 1,610,916 + SGL_RS09390 0.20 -0.5 1,610,918 - SGL_RS09390 0.21 -0.3 1,610,919 - SGL_RS09390 0.21 +0.1 1,611,029 - SGL_RS09390 0.37 -0.6 1,611,046 + SGL_RS09390 0.39 +0.1 1,611,075 - SGL_RS09390 0.43 -0.1 1,611,174 - SGL_RS09390 0.58 -0.0 1,611,182 + SGL_RS09390 0.59 -0.3 1,611,189 - SGL_RS09390 0.60 -0.5 1,611,198 + SGL_RS09390 0.61 -0.5 1,611,203 + SGL_RS09390 0.62 +0.3 1,611,224 - SGL_RS09390 0.65 +0.2 1,611,321 + SGL_RS09390 0.79 +0.2 1,611,421 + -0.0 1,611,748 + SGL_RS09395 0.16 -1.2
Or see this region's nucleotide sequence