Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09280 and SGL_RS09285 are separated by 9 nucleotides SGL_RS09285 and SGL_RS09290 are separated by 72 nucleotides SGL_RS09290 and SGL_RS09295 are separated by 113 nucleotides
SGL_RS09280: SGL_RS09280 - DUF3084 domain-containing protein, at 1,587,899 to 1,589,374
_RS09280
SGL_RS09285: SGL_RS09285 - global nitrogen regulator NtcA, at 1,589,384 to 1,590,061
_RS09285
SGL_RS09290: SGL_RS09290 - hypothetical protein, at 1,590,134 to 1,590,382
_RS09290
SGL_RS09295: SGL_RS09295 - alpha/beta hydrolase, at 1,590,496 to 1,592,364
_RS09295
Position (kb)
1590
1591 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1
2 at 1590.117 kb on + strand at 1590.142 kb on + strand at 1590.205 kb on - strand, within SGL_RS09290 at 1590.289 kb on - strand, within SGL_RS09290 at 1590.456 kb on + strand at 1590.581 kb on - strand at 1590.664 kb on - strand at 1590.900 kb on + strand, within SGL_RS09295 at 1590.906 kb on - strand, within SGL_RS09295 at 1590.906 kb on - strand, within SGL_RS09295 at 1591.044 kb on - strand, within SGL_RS09295 at 1591.063 kb on - strand, within SGL_RS09295 at 1591.063 kb on - strand, within SGL_RS09295 at 1591.119 kb on - strand, within SGL_RS09295 at 1591.157 kb on - strand, within SGL_RS09295 at 1591.227 kb on + strand, within SGL_RS09295 at 1591.227 kb on + strand, within SGL_RS09295
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,590,117 + -0.1 1,590,142 + +1.2 1,590,205 - SGL_RS09290 0.29 +1.9 1,590,289 - SGL_RS09290 0.62 +1.7 1,590,456 + -0.2 1,590,581 - -1.9 1,590,664 - -4.9 1,590,900 + SGL_RS09295 0.22 -5.5 1,590,906 - SGL_RS09295 0.22 -6.0 1,590,906 - SGL_RS09295 0.22 -5.8 1,591,044 - SGL_RS09295 0.29 -5.5 1,591,063 - SGL_RS09295 0.30 -5.3 1,591,063 - SGL_RS09295 0.30 -6.0 1,591,119 - SGL_RS09295 0.33 -7.1 1,591,157 - SGL_RS09295 0.35 -6.2 1,591,227 + SGL_RS09295 0.39 -5.9 1,591,227 + SGL_RS09295 0.39 -6.1
Or see this region's nucleotide sequence