Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08105 and SGL_RS08110 are separated by 119 nucleotides SGL_RS08110 and SGL_RS08115 are separated by 49 nucleotides SGL_RS08115 and SGL_RS08120 are separated by 284 nucleotides
SGL_RS08105: SGL_RS08105 - hypothetical protein, at 1,329,388 to 1,329,990
_RS08105
SGL_RS08110: SGL_RS08110 - type 1 glutamine amidotransferase, at 1,330,110 to 1,330,880
_RS08110
SGL_RS08115: SGL_RS08115 - NAD(P)/FAD-dependent oxidoreductase, at 1,330,930 to 1,332,144
_RS08115
SGL_RS08120: SGL_RS08120 - N-acetylmuramoyl-L-alanine amidase, at 1,332,429 to 1,334,378
_RS08120
Position (kb)
1330
1331
1332
1333 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1329.937 kb on - strand at 1329.958 kb on + strand at 1330.884 kb on + strand at 1330.926 kb on + strand at 1330.980 kb on + strand at 1331.010 kb on + strand at 1331.028 kb on - strand at 1331.029 kb on - strand at 1331.029 kb on - strand at 1331.212 kb on + strand, within SGL_RS08115 at 1331.220 kb on - strand, within SGL_RS08115 at 1331.237 kb on - strand, within SGL_RS08115 at 1331.275 kb on - strand, within SGL_RS08115 at 1331.285 kb on + strand, within SGL_RS08115 at 1331.347 kb on - strand, within SGL_RS08115 at 1331.505 kb on - strand, within SGL_RS08115 at 1331.541 kb on - strand, within SGL_RS08115 at 1331.661 kb on - strand, within SGL_RS08115 at 1331.991 kb on - strand, within SGL_RS08115 at 1332.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,329,937 - -0.3 1,329,958 + -0.1 1,330,884 + +0.5 1,330,926 + -0.4 1,330,980 + -4.3 1,331,010 + -3.6 1,331,028 - -1.9 1,331,029 - -2.4 1,331,029 - -2.4 1,331,212 + SGL_RS08115 0.23 -2.5 1,331,220 - SGL_RS08115 0.24 -2.7 1,331,237 - SGL_RS08115 0.25 -2.6 1,331,275 - SGL_RS08115 0.28 -3.4 1,331,285 + SGL_RS08115 0.29 -3.0 1,331,347 - SGL_RS08115 0.34 -3.3 1,331,505 - SGL_RS08115 0.47 -1.6 1,331,541 - SGL_RS08115 0.50 -2.2 1,331,661 - SGL_RS08115 0.60 -3.2 1,331,991 - SGL_RS08115 0.87 -3.0 1,332,208 + -0.2
Or see this region's nucleotide sequence