Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08085 and SGL_RS08090 are separated by 253 nucleotides SGL_RS08090 and SGL_RS08095 are separated by 118 nucleotides SGL_RS08095 and SGL_RS08100 are separated by 47 nucleotides SGL_RS08100 and SGL_RS08105 are separated by 151 nucleotides SGL_RS08105 and SGL_RS08110 are separated by 119 nucleotides
SGL_RS08085: SGL_RS08085 - DUF1499 domain-containing protein, at 1,326,150 to 1,326,626
_RS08085
SGL_RS08090: SGL_RS08090 - photosystem II reaction center protein Psb28, at 1,326,880 to 1,327,248
_RS08090
SGL_RS08095: SGL_RS08095 - peptide ABC transporter substrate-binding protein, at 1,327,367 to 1,329,115
_RS08095
SGL_RS08100: SGL_RS08100 - tRNA-Asp, at 1,329,163 to 1,329,236
_RS08100
SGL_RS08105: SGL_RS08105 - hypothetical protein, at 1,329,388 to 1,329,990
_RS08105
SGL_RS08110: SGL_RS08110 - type 1 glutamine amidotransferase, at 1,330,110 to 1,330,880
_RS08110
Position (kb)
1327
1328
1329
1330 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1326.455 kb on + strand, within SGL_RS08085 at 1326.575 kb on - strand, within SGL_RS08085 at 1326.652 kb on + strand at 1326.735 kb on + strand at 1326.791 kb on + strand at 1326.793 kb on + strand at 1326.835 kb on + strand at 1326.887 kb on + strand at 1326.946 kb on - strand, within SGL_RS08090 at 1326.979 kb on + strand, within SGL_RS08090 at 1326.987 kb on - strand, within SGL_RS08090 at 1327.017 kb on + strand, within SGL_RS08090 at 1327.395 kb on + strand at 1327.484 kb on + strand at 1327.789 kb on + strand, within SGL_RS08095 at 1327.797 kb on - strand, within SGL_RS08095 at 1327.882 kb on + strand, within SGL_RS08095 at 1328.197 kb on + strand, within SGL_RS08095 at 1328.214 kb on + strand, within SGL_RS08095 at 1328.257 kb on - strand, within SGL_RS08095 at 1328.398 kb on + strand, within SGL_RS08095 at 1328.424 kb on - strand, within SGL_RS08095 at 1328.684 kb on - strand, within SGL_RS08095 at 1328.779 kb on + strand, within SGL_RS08095 at 1329.012 kb on + strand at 1329.027 kb on + strand at 1329.131 kb on - strand at 1329.281 kb on + strand at 1329.361 kb on - strand at 1329.362 kb on - strand at 1329.471 kb on + strand, within SGL_RS08105 at 1329.471 kb on + strand, within SGL_RS08105 at 1329.471 kb on + strand, within SGL_RS08105 at 1329.544 kb on - strand, within SGL_RS08105 at 1329.556 kb on + strand, within SGL_RS08105 at 1329.610 kb on + strand, within SGL_RS08105 at 1329.694 kb on + strand, within SGL_RS08105 at 1329.832 kb on - strand, within SGL_RS08105 at 1329.864 kb on + strand, within SGL_RS08105 at 1329.871 kb on + strand, within SGL_RS08105 at 1329.937 kb on - strand at 1329.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,326,455 + SGL_RS08085 0.64 -0.8 1,326,575 - SGL_RS08085 0.89 -0.3 1,326,652 + -0.8 1,326,735 + -0.6 1,326,791 + +0.3 1,326,793 + +0.1 1,326,835 + -0.0 1,326,887 + -0.2 1,326,946 - SGL_RS08090 0.18 -0.1 1,326,979 + SGL_RS08090 0.27 +0.3 1,326,987 - SGL_RS08090 0.29 -0.6 1,327,017 + SGL_RS08090 0.37 +0.1 1,327,395 + -3.6 1,327,484 + -5.1 1,327,789 + SGL_RS08095 0.24 -5.7 1,327,797 - SGL_RS08095 0.25 -5.0 1,327,882 + SGL_RS08095 0.29 -4.1 1,328,197 + SGL_RS08095 0.47 -6.0 1,328,214 + SGL_RS08095 0.48 -5.8 1,328,257 - SGL_RS08095 0.51 -6.3 1,328,398 + SGL_RS08095 0.59 -5.5 1,328,424 - SGL_RS08095 0.60 -5.1 1,328,684 - SGL_RS08095 0.75 -5.9 1,328,779 + SGL_RS08095 0.81 -4.9 1,329,012 + -5.2 1,329,027 + -5.5 1,329,131 - +0.2 1,329,281 + +0.2 1,329,361 - +0.5 1,329,362 - +0.4 1,329,471 + SGL_RS08105 0.14 -0.1 1,329,471 + SGL_RS08105 0.14 -0.5 1,329,471 + SGL_RS08105 0.14 -0.3 1,329,544 - SGL_RS08105 0.26 -1.0 1,329,556 + SGL_RS08105 0.28 -0.2 1,329,610 + SGL_RS08105 0.37 -0.5 1,329,694 + SGL_RS08105 0.51 +0.7 1,329,832 - SGL_RS08105 0.74 -0.1 1,329,864 + SGL_RS08105 0.79 -0.1 1,329,871 + SGL_RS08105 0.80 -0.2 1,329,937 - -0.3 1,329,958 + -0.1
Or see this region's nucleotide sequence