Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07855 and SGL_RS07860 are separated by 24 nucleotides SGL_RS07860 and SGL_RS07865 are separated by 23 nucleotides SGL_RS07865 and SGL_RS07870 overlap by 4 nucleotides SGL_RS07870 and SGL_RS07875 are separated by 38 nucleotides
SGL_RS07855: SGL_RS07855 - MASE1 domain-containing protein, at 1,278,326 to 1,280,542
_RS07855
SGL_RS07860: SGL_RS07860 - SDR family NAD(P)-dependent oxidoreductase, at 1,280,567 to 1,281,337
_RS07860
SGL_RS07865: SGL_RS07865 - signal peptide peptidase SppA, at 1,281,361 to 1,283,193
_RS07865
SGL_RS07870: SGL_RS07870 - Ycf51 family protein, at 1,283,190 to 1,283,702
_RS07870
SGL_RS07875: SGL_RS07875 - alpha/beta hydrolase, at 1,283,741 to 1,284,526
_RS07875
Position (kb)
1281
1282
1283
1284 Strain fitness (log2 ratio)
-2
-1
0
1 at 1280.396 kb on - strand at 1280.426 kb on + strand at 1280.436 kb on + strand at 1280.527 kb on + strand at 1280.587 kb on + strand at 1280.706 kb on + strand, within SGL_RS07860 at 1280.860 kb on + strand, within SGL_RS07860 at 1280.868 kb on - strand, within SGL_RS07860 at 1280.995 kb on + strand, within SGL_RS07860 at 1281.502 kb on - strand at 1281.683 kb on + strand, within SGL_RS07865 at 1281.688 kb on + strand, within SGL_RS07865 at 1281.703 kb on + strand, within SGL_RS07865 at 1281.703 kb on + strand, within SGL_RS07865 at 1281.865 kb on + strand, within SGL_RS07865 at 1281.943 kb on + strand, within SGL_RS07865 at 1282.123 kb on - strand, within SGL_RS07865 at 1282.142 kb on + strand, within SGL_RS07865 at 1282.493 kb on - strand, within SGL_RS07865 at 1282.596 kb on - strand, within SGL_RS07865 at 1282.630 kb on + strand, within SGL_RS07865 at 1282.975 kb on - strand, within SGL_RS07865 at 1283.006 kb on - strand, within SGL_RS07865 at 1283.012 kb on - strand at 1283.224 kb on + strand at 1283.301 kb on + strand, within SGL_RS07870 at 1283.302 kb on + strand, within SGL_RS07870 at 1283.320 kb on - strand, within SGL_RS07870 at 1283.538 kb on + strand, within SGL_RS07870 at 1283.539 kb on + strand, within SGL_RS07870 at 1283.547 kb on - strand, within SGL_RS07870 at 1283.762 kb on - strand at 1283.849 kb on + strand, within SGL_RS07875 at 1283.857 kb on + strand, within SGL_RS07875 at 1283.891 kb on - strand, within SGL_RS07875 at 1283.893 kb on + strand, within SGL_RS07875 at 1283.917 kb on + strand, within SGL_RS07875 at 1283.920 kb on - strand, within SGL_RS07875 at 1283.924 kb on + strand, within SGL_RS07875 at 1283.924 kb on + strand, within SGL_RS07875 at 1283.925 kb on - strand, within SGL_RS07875 at 1283.955 kb on + strand, within SGL_RS07875 at 1283.977 kb on - strand, within SGL_RS07875 at 1284.000 kb on + strand, within SGL_RS07875 at 1284.018 kb on - strand, within SGL_RS07875 at 1284.018 kb on - strand, within SGL_RS07875 at 1284.018 kb on - strand, within SGL_RS07875 at 1284.071 kb on + strand, within SGL_RS07875 at 1284.095 kb on + strand, within SGL_RS07875 at 1284.097 kb on - strand, within SGL_RS07875 at 1284.185 kb on - strand, within SGL_RS07875
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,280,396 - -1.7 1,280,426 + -2.2 1,280,436 + -2.3 1,280,527 + +0.5 1,280,587 + +0.2 1,280,706 + SGL_RS07860 0.18 +0.3 1,280,860 + SGL_RS07860 0.38 +0.3 1,280,868 - SGL_RS07860 0.39 -0.2 1,280,995 + SGL_RS07860 0.56 +0.3 1,281,502 - -0.6 1,281,683 + SGL_RS07865 0.18 -0.4 1,281,688 + SGL_RS07865 0.18 -0.4 1,281,703 + SGL_RS07865 0.19 -1.2 1,281,703 + SGL_RS07865 0.19 +0.6 1,281,865 + SGL_RS07865 0.27 +0.0 1,281,943 + SGL_RS07865 0.32 -0.2 1,282,123 - SGL_RS07865 0.42 -0.9 1,282,142 + SGL_RS07865 0.43 -0.6 1,282,493 - SGL_RS07865 0.62 -0.4 1,282,596 - SGL_RS07865 0.67 +0.1 1,282,630 + SGL_RS07865 0.69 -0.5 1,282,975 - SGL_RS07865 0.88 -0.7 1,283,006 - SGL_RS07865 0.90 -1.7 1,283,012 - -0.3 1,283,224 + -0.9 1,283,301 + SGL_RS07870 0.22 -0.1 1,283,302 + SGL_RS07870 0.22 -0.3 1,283,320 - SGL_RS07870 0.25 +0.7 1,283,538 + SGL_RS07870 0.68 -1.1 1,283,539 + SGL_RS07870 0.68 +1.0 1,283,547 - SGL_RS07870 0.70 +0.3 1,283,762 - +0.2 1,283,849 + SGL_RS07875 0.14 +0.1 1,283,857 + SGL_RS07875 0.15 -0.1 1,283,891 - SGL_RS07875 0.19 -0.3 1,283,893 + SGL_RS07875 0.19 -0.6 1,283,917 + SGL_RS07875 0.22 -0.0 1,283,920 - SGL_RS07875 0.23 -0.3 1,283,924 + SGL_RS07875 0.23 +0.0 1,283,924 + SGL_RS07875 0.23 +0.2 1,283,925 - SGL_RS07875 0.23 -0.5 1,283,955 + SGL_RS07875 0.27 +0.1 1,283,977 - SGL_RS07875 0.30 -0.1 1,284,000 + SGL_RS07875 0.33 +0.3 1,284,018 - SGL_RS07875 0.35 -0.1 1,284,018 - SGL_RS07875 0.35 +0.1 1,284,018 - SGL_RS07875 0.35 +0.1 1,284,071 + SGL_RS07875 0.42 +0.2 1,284,095 + SGL_RS07875 0.45 -0.2 1,284,097 - SGL_RS07875 0.45 +0.9 1,284,185 - SGL_RS07875 0.56 +0.3
Or see this region's nucleotide sequence