Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07575 and SGL_RS07580 are separated by 86 nucleotides SGL_RS07580 and SGL_RS07585 are separated by 119 nucleotides SGL_RS07585 and SGL_RS07590 are separated by 107 nucleotides
SGL_RS07575: SGL_RS07575 - D-alanine--D-alanine ligase family protein, at 1,224,777 to 1,225,841
_RS07575
SGL_RS07580: SGL_RS07580 - exopolysaccharide biosynthesis protein, at 1,225,928 to 1,226,566
_RS07580
SGL_RS07585: SGL_RS07585 - ATP-dependent Clp protease adapter ClpS, at 1,226,686 to 1,226,982
_RS07585
SGL_RS07590: SGL_RS07590 - hydrogenase maturation protease, at 1,227,090 to 1,227,563
_RS07590
Position (kb) 1225
1226
1227 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1226.003 kb on + strand, within SGL_RS07580 at 1226.204 kb on + strand, within SGL_RS07580 at 1226.204 kb on + strand, within SGL_RS07580 at 1226.233 kb on - strand, within SGL_RS07580 at 1226.233 kb on - strand, within SGL_RS07580 at 1226.252 kb on - strand, within SGL_RS07580 at 1226.313 kb on + strand, within SGL_RS07580 at 1226.568 kb on + strand at 1226.807 kb on + strand, within SGL_RS07585 at 1226.860 kb on + strand, within SGL_RS07585 at 1226.932 kb on - strand, within SGL_RS07585 at 1226.944 kb on - strand, within SGL_RS07585 at 1226.977 kb on + strand at 1226.999 kb on + strand at 1227.141 kb on - strand, within SGL_RS07590 at 1227.170 kb on - strand, within SGL_RS07590 at 1227.240 kb on + strand, within SGL_RS07590 at 1227.243 kb on - strand, within SGL_RS07590 at 1227.261 kb on + strand, within SGL_RS07590 at 1227.320 kb on + strand, within SGL_RS07590 at 1227.407 kb on - strand, within SGL_RS07590 at 1227.434 kb on + strand, within SGL_RS07590 at 1227.450 kb on + strand, within SGL_RS07590 at 1227.509 kb on + strand, within SGL_RS07590
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,226,003 + SGL_RS07580 0.12 -3.3 1,226,204 + SGL_RS07580 0.43 -3.1 1,226,204 + SGL_RS07580 0.43 -3.3 1,226,233 - SGL_RS07580 0.48 -4.1 1,226,233 - SGL_RS07580 0.48 -2.6 1,226,252 - SGL_RS07580 0.51 -3.9 1,226,313 + SGL_RS07580 0.60 -3.1 1,226,568 + +0.2 1,226,807 + SGL_RS07585 0.41 +0.4 1,226,860 + SGL_RS07585 0.59 +0.3 1,226,932 - SGL_RS07585 0.83 +0.4 1,226,944 - SGL_RS07585 0.87 +0.6 1,226,977 + +0.0 1,226,999 + +0.2 1,227,141 - SGL_RS07590 0.11 +0.7 1,227,170 - SGL_RS07590 0.17 +0.3 1,227,240 + SGL_RS07590 0.32 -0.0 1,227,243 - SGL_RS07590 0.32 -0.3 1,227,261 + SGL_RS07590 0.36 +0.5 1,227,320 + SGL_RS07590 0.49 +0.1 1,227,407 - SGL_RS07590 0.67 +0.6 1,227,434 + SGL_RS07590 0.73 -0.4 1,227,450 + SGL_RS07590 0.76 -0.3 1,227,509 + SGL_RS07590 0.88 -0.5
Or see this region's nucleotide sequence