Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07540 and SGL_RS07545 are separated by 29 nucleotides SGL_RS07545 and SGL_RS07550 are separated by 54 nucleotides SGL_RS07550 and SGL_RS07555 are separated by 59 nucleotides SGL_RS07555 and SGL_RS07560 are separated by 137 nucleotides SGL_RS07560 and SGL_RS07565 are separated by 9 nucleotides
SGL_RS07540: SGL_RS07540 - endonuclease MutS2, at 1,217,156 to 1,219,624
_RS07540
SGL_RS07545: SGL_RS07545 - serine/threonine-protein phosphatase, at 1,219,654 to 1,220,418
_RS07545
SGL_RS07550: SGL_RS07550 - AarF/ABC1/UbiB kinase family protein, at 1,220,473 to 1,222,230
_RS07550
SGL_RS07555: SGL_RS07555 - hypothetical protein, at 1,222,290 to 1,222,472
_RS07555
SGL_RS07560: SGL_RS07560 - thylakoid lumen protein, at 1,222,610 to 1,222,927
_RS07560
SGL_RS07565: SGL_RS07565 - hypothetical protein, at 1,222,937 to 1,223,209
_RS07565
Position (kb) 1220
1221
1222
1223 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 1219.632 kb on - strand at 1219.634 kb on - strand at 1219.660 kb on + strand at 1219.671 kb on + strand at 1219.765 kb on + strand, within SGL_RS07545 at 1219.833 kb on + strand, within SGL_RS07545 at 1219.833 kb on + strand, within SGL_RS07545 at 1219.841 kb on - strand, within SGL_RS07545 at 1219.928 kb on + strand, within SGL_RS07545 at 1219.934 kb on + strand, within SGL_RS07545 at 1219.942 kb on - strand, within SGL_RS07545 at 1219.957 kb on - strand, within SGL_RS07545 at 1220.109 kb on - strand, within SGL_RS07545 at 1220.260 kb on + strand, within SGL_RS07545 at 1220.322 kb on - strand, within SGL_RS07545 at 1220.465 kb on - strand at 1220.595 kb on - strand at 1220.659 kb on - strand, within SGL_RS07550 at 1220.690 kb on - strand, within SGL_RS07550 at 1220.695 kb on - strand, within SGL_RS07550 at 1220.732 kb on - strand, within SGL_RS07550 at 1220.763 kb on - strand, within SGL_RS07550 at 1220.828 kb on - strand, within SGL_RS07550 at 1221.002 kb on - strand, within SGL_RS07550 at 1221.089 kb on - strand, within SGL_RS07550 at 1221.108 kb on - strand, within SGL_RS07550 at 1221.198 kb on + strand, within SGL_RS07550 at 1221.206 kb on - strand, within SGL_RS07550 at 1221.301 kb on + strand, within SGL_RS07550 at 1221.717 kb on - strand, within SGL_RS07550 at 1221.722 kb on - strand, within SGL_RS07550 at 1221.727 kb on - strand, within SGL_RS07550 at 1221.778 kb on - strand, within SGL_RS07550 at 1221.804 kb on - strand, within SGL_RS07550 at 1221.926 kb on + strand, within SGL_RS07550 at 1221.942 kb on + strand, within SGL_RS07550 at 1221.942 kb on + strand, within SGL_RS07550 at 1222.415 kb on + strand, within SGL_RS07555 at 1222.613 kb on + strand at 1222.644 kb on + strand, within SGL_RS07560 at 1222.652 kb on - strand, within SGL_RS07560 at 1222.679 kb on - strand, within SGL_RS07560 at 1222.774 kb on - strand, within SGL_RS07560 at 1222.853 kb on + strand, within SGL_RS07560 at 1222.863 kb on - strand, within SGL_RS07560 at 1222.885 kb on + strand, within SGL_RS07560 at 1222.986 kb on + strand, within SGL_RS07565 at 1223.023 kb on - strand, within SGL_RS07565 at 1223.040 kb on + strand, within SGL_RS07565 at 1223.048 kb on - strand, within SGL_RS07565 at 1223.114 kb on + strand, within SGL_RS07565 at 1223.122 kb on - strand, within SGL_RS07565 at 1223.122 kb on - strand, within SGL_RS07565 at 1223.132 kb on + strand, within SGL_RS07565 at 1223.158 kb on - strand, within SGL_RS07565
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,219,632 - -0.7 1,219,634 - +0.4 1,219,660 + +0.3 1,219,671 + -1.6 1,219,765 + SGL_RS07545 0.15 -2.6 1,219,833 + SGL_RS07545 0.23 -3.8 1,219,833 + SGL_RS07545 0.23 -3.0 1,219,841 - SGL_RS07545 0.24 -3.1 1,219,928 + SGL_RS07545 0.36 -1.1 1,219,934 + SGL_RS07545 0.37 -2.8 1,219,942 - SGL_RS07545 0.38 -2.4 1,219,957 - SGL_RS07545 0.40 -0.0 1,220,109 - SGL_RS07545 0.59 -4.7 1,220,260 + SGL_RS07545 0.79 -3.2 1,220,322 - SGL_RS07545 0.87 -3.1 1,220,465 - -1.6 1,220,595 - -0.2 1,220,659 - SGL_RS07550 0.11 -0.0 1,220,690 - SGL_RS07550 0.12 +0.5 1,220,695 - SGL_RS07550 0.13 +0.7 1,220,732 - SGL_RS07550 0.15 +0.6 1,220,763 - SGL_RS07550 0.16 -0.1 1,220,828 - SGL_RS07550 0.20 -0.2 1,221,002 - SGL_RS07550 0.30 +0.4 1,221,089 - SGL_RS07550 0.35 +1.0 1,221,108 - SGL_RS07550 0.36 +0.4 1,221,198 + SGL_RS07550 0.41 +0.4 1,221,206 - SGL_RS07550 0.42 +0.0 1,221,301 + SGL_RS07550 0.47 -0.2 1,221,717 - SGL_RS07550 0.71 +0.5 1,221,722 - SGL_RS07550 0.71 +0.3 1,221,727 - SGL_RS07550 0.71 +0.2 1,221,778 - SGL_RS07550 0.74 +0.5 1,221,804 - SGL_RS07550 0.76 +0.3 1,221,926 + SGL_RS07550 0.83 -0.1 1,221,942 + SGL_RS07550 0.84 +0.0 1,221,942 + SGL_RS07550 0.84 +0.2 1,222,415 + SGL_RS07555 0.68 -0.1 1,222,613 + +1.5 1,222,644 + SGL_RS07560 0.11 +0.4 1,222,652 - SGL_RS07560 0.13 +0.5 1,222,679 - SGL_RS07560 0.22 +2.6 1,222,774 - SGL_RS07560 0.52 +1.1 1,222,853 + SGL_RS07560 0.76 +0.8 1,222,863 - SGL_RS07560 0.80 +1.2 1,222,885 + SGL_RS07560 0.86 +1.5 1,222,986 + SGL_RS07565 0.18 -4.1 1,223,023 - SGL_RS07565 0.32 -5.8 1,223,040 + SGL_RS07565 0.38 -6.5 1,223,048 - SGL_RS07565 0.41 -6.9 1,223,114 + SGL_RS07565 0.65 -4.5 1,223,122 - SGL_RS07565 0.68 -5.3 1,223,122 - SGL_RS07565 0.68 -3.3 1,223,132 + SGL_RS07565 0.71 -4.9 1,223,158 - SGL_RS07565 0.81 -5.8
Or see this region's nucleotide sequence