Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07175 and SGL_RS07180 are separated by 22 nucleotides SGL_RS07180 and SGL_RS18765 are separated by 157 nucleotides SGL_RS18765 and SGL_RS07185 are separated by 154 nucleotides SGL_RS07185 and SGL_RS19815 are separated by 88 nucleotides
SGL_RS07175: SGL_RS07175 - NFACT family protein, at 1,138,702 to 1,140,474
_RS07175
SGL_RS07180: SGL_RS07180 - carboxynorspermidine decarboxylase, at 1,140,497 to 1,141,645
_RS07180
SGL_RS18765: SGL_RS18765 - chlorophyll A-B-binding protein, at 1,141,803 to 1,141,946
_RS18765
SGL_RS07185: SGL_RS07185 - hypothetical protein, at 1,142,101 to 1,142,412
_RS07185
SGL_RS19815: SGL_RS19815 - hypothetical protein, at 1,142,501 to 1,142,707
_RS19815
Position (kb)
1140
1141
1142 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 1139.508 kb on + strand, within SGL_RS07175 at 1139.689 kb on - strand, within SGL_RS07175 at 1139.735 kb on - strand, within SGL_RS07175 at 1139.767 kb on + strand, within SGL_RS07175 at 1139.775 kb on - strand, within SGL_RS07175 at 1139.937 kb on - strand, within SGL_RS07175 at 1140.095 kb on + strand, within SGL_RS07175 at 1140.113 kb on + strand, within SGL_RS07175 at 1140.121 kb on - strand, within SGL_RS07175 at 1140.233 kb on + strand, within SGL_RS07175 at 1140.241 kb on - strand, within SGL_RS07175 at 1140.343 kb on - strand at 1140.372 kb on - strand at 1140.392 kb on - strand at 1140.460 kb on - strand at 1140.594 kb on + strand at 1140.594 kb on + strand at 1140.688 kb on - strand, within SGL_RS07180 at 1140.997 kb on - strand, within SGL_RS07180 at 1141.026 kb on + strand, within SGL_RS07180 at 1141.145 kb on - strand, within SGL_RS07180 at 1141.325 kb on - strand, within SGL_RS07180 at 1141.457 kb on + strand, within SGL_RS07180 at 1141.516 kb on + strand, within SGL_RS07180 at 1141.525 kb on - strand, within SGL_RS07180 at 1141.701 kb on - strand at 1141.854 kb on + strand, within SGL_RS18765 at 1141.949 kb on - strand at 1142.065 kb on - strand at 1142.099 kb on - strand at 1142.099 kb on - strand at 1142.137 kb on + strand, within SGL_RS07185 at 1142.165 kb on - strand, within SGL_RS07185 at 1142.165 kb on - strand, within SGL_RS07185 at 1142.185 kb on + strand, within SGL_RS07185
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,139,508 + SGL_RS07175 0.45 +1.1 1,139,689 - SGL_RS07175 0.56 +1.7 1,139,735 - SGL_RS07175 0.58 +1.4 1,139,767 + SGL_RS07175 0.60 +1.0 1,139,775 - SGL_RS07175 0.61 +1.3 1,139,937 - SGL_RS07175 0.70 +1.3 1,140,095 + SGL_RS07175 0.79 +1.9 1,140,113 + SGL_RS07175 0.80 +3.2 1,140,121 - SGL_RS07175 0.80 +1.6 1,140,233 + SGL_RS07175 0.86 +1.3 1,140,241 - SGL_RS07175 0.87 +1.5 1,140,343 - +1.1 1,140,372 - +1.6 1,140,392 - +1.5 1,140,460 - -1.1 1,140,594 + -3.8 1,140,594 + -4.2 1,140,688 - SGL_RS07180 0.17 -1.1 1,140,997 - SGL_RS07180 0.44 -1.6 1,141,026 + SGL_RS07180 0.46 -4.8 1,141,145 - SGL_RS07180 0.56 -2.4 1,141,325 - SGL_RS07180 0.72 -3.4 1,141,457 + SGL_RS07180 0.84 -0.6 1,141,516 + SGL_RS07180 0.89 -3.0 1,141,525 - SGL_RS07180 0.89 -6.1 1,141,701 - +0.1 1,141,854 + SGL_RS18765 0.35 -0.2 1,141,949 - +0.2 1,142,065 - +0.2 1,142,099 - +0.3 1,142,099 - -0.5 1,142,137 + SGL_RS07185 0.12 +0.3 1,142,165 - SGL_RS07185 0.21 -0.6 1,142,165 - SGL_RS07185 0.21 -0.4 1,142,185 + SGL_RS07185 0.27 +0.1
Or see this region's nucleotide sequence