Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07125 and SGL_RS07130 are separated by 305 nucleotides SGL_RS07130 and SGL_RS07135 are separated by 126 nucleotides SGL_RS07135 and SGL_RS07140 are separated by 16 nucleotides
SGL_RS07125: SGL_RS07125 - translocation/assembly module TamB domain-containing protein, at 1,124,071 to 1,130,007
_RS07125
SGL_RS07130: SGL_RS07130 - AAA-like domain-containing protein, at 1,130,313 to 1,131,683
_RS07130
SGL_RS07135: SGL_RS07135 - large conductance mechanosensitive channel protein MscL, at 1,131,810 to 1,132,247
_RS07135
SGL_RS07140: SGL_RS07140 - Holliday junction branch migration protein RuvA, at 1,132,264 to 1,132,899
_RS07140
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 1129.427 kb on + strand at 1129.434 kb on - strand at 1129.435 kb on - strand at 1129.468 kb on + strand at 1129.580 kb on + strand at 1129.589 kb on - strand at 1129.796 kb on + strand at 1129.876 kb on - strand at 1129.887 kb on + strand at 1129.995 kb on + strand at 1130.058 kb on + strand at 1130.061 kb on - strand at 1130.062 kb on - strand at 1130.063 kb on + strand at 1130.692 kb on + strand, within SGL_RS07130 at 1130.816 kb on - strand, within SGL_RS07130 at 1131.024 kb on - strand, within SGL_RS07130 at 1131.130 kb on - strand, within SGL_RS07130 at 1131.222 kb on + strand, within SGL_RS07130 at 1131.246 kb on - strand, within SGL_RS07130 at 1131.301 kb on + strand, within SGL_RS07130 at 1131.398 kb on + strand, within SGL_RS07130 at 1131.406 kb on - strand, within SGL_RS07130 at 1131.434 kb on + strand, within SGL_RS07130 at 1131.611 kb on + strand at 1131.978 kb on + strand, within SGL_RS07135 at 1131.986 kb on - strand, within SGL_RS07135 at 1131.986 kb on - strand, within SGL_RS07135 at 1132.147 kb on + strand, within SGL_RS07135
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 1,129,427 + -1.3 1,129,434 - -0.8 1,129,435 - -0.7 1,129,468 + -1.8 1,129,580 + -0.2 1,129,589 - -1.4 1,129,796 + -1.2 1,129,876 - -2.0 1,129,887 + -1.9 1,129,995 + -1.2 1,130,058 + -1.4 1,130,061 - +0.1 1,130,062 - -0.5 1,130,063 + -0.9 1,130,692 + SGL_RS07130 0.28 -8.4 1,130,816 - SGL_RS07130 0.37 -6.0 1,131,024 - SGL_RS07130 0.52 -7.3 1,131,130 - SGL_RS07130 0.60 -5.3 1,131,222 + SGL_RS07130 0.66 -7.5 1,131,246 - SGL_RS07130 0.68 -6.4 1,131,301 + SGL_RS07130 0.72 -6.8 1,131,398 + SGL_RS07130 0.79 -6.8 1,131,406 - SGL_RS07130 0.80 -5.8 1,131,434 + SGL_RS07130 0.82 -7.6 1,131,611 + -7.2 1,131,978 + SGL_RS07135 0.38 +0.9 1,131,986 - SGL_RS07135 0.40 -0.5 1,131,986 - SGL_RS07135 0.40 +1.0 1,132,147 + SGL_RS07135 0.77 +0.3
Or see this region's nucleotide sequence