Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06015 and SGL_RS06020 are separated by 115 nucleotides SGL_RS06020 and SGL_RS06025 are separated by 109 nucleotides SGL_RS06025 and SGL_RS06030 are separated by 204 nucleotides
SGL_RS06015: SGL_RS06015 - urea ABC transporter permease subunit UrtC, at 883,287 to 884,519
_RS06015
SGL_RS06020: SGL_RS06020 - sugar ABC transporter permease, at 884,635 to 885,531
_RS06020
SGL_RS06025: SGL_RS06025 - DUF29 domain-containing protein, at 885,641 to 886,078
_RS06025
SGL_RS06030: SGL_RS06030 - HhoA/HhoB/HtrA family serine endopeptidase, at 886,283 to 887,542
_RS06030
Position (kb) 884
885
886 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 883.718 kb on + strand, within SGL_RS06015 at 883.752 kb on + strand, within SGL_RS06015 at 884.005 kb on - strand, within SGL_RS06015 at 884.053 kb on + strand, within SGL_RS06015 at 884.091 kb on - strand, within SGL_RS06015 at 884.129 kb on + strand, within SGL_RS06015 at 884.137 kb on - strand, within SGL_RS06015 at 884.138 kb on + strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.146 kb on - strand, within SGL_RS06015 at 884.156 kb on - strand, within SGL_RS06015 at 884.167 kb on - strand, within SGL_RS06015 at 884.169 kb on - strand, within SGL_RS06015 at 884.209 kb on + strand, within SGL_RS06015 at 884.238 kb on - strand, within SGL_RS06015 at 884.240 kb on + strand, within SGL_RS06015 at 884.268 kb on - strand, within SGL_RS06015 at 884.280 kb on - strand, within SGL_RS06015 at 884.363 kb on - strand, within SGL_RS06015 at 884.398 kb on - strand at 884.500 kb on - strand at 884.504 kb on + strand at 884.512 kb on - strand at 884.512 kb on - strand at 884.671 kb on - strand at 884.672 kb on - strand at 884.971 kb on + strand, within SGL_RS06020 at 884.971 kb on + strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 884.979 kb on - strand, within SGL_RS06020 at 885.112 kb on - strand, within SGL_RS06020 at 885.159 kb on - strand, within SGL_RS06020 at 885.446 kb on - strand at 885.527 kb on + strand at 885.902 kb on - strand, within SGL_RS06025 at 886.264 kb on + strand at 886.280 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 883,718 + SGL_RS06015 0.35 -0.2 883,752 + SGL_RS06015 0.38 -0.0 884,005 - SGL_RS06015 0.58 +0.1 884,053 + SGL_RS06015 0.62 +0.4 884,091 - SGL_RS06015 0.65 -0.4 884,129 + SGL_RS06015 0.68 +0.4 884,137 - SGL_RS06015 0.69 +0.7 884,138 + SGL_RS06015 0.69 -0.0 884,146 - SGL_RS06015 0.70 -0.2 884,146 - SGL_RS06015 0.70 +0.3 884,146 - SGL_RS06015 0.70 +0.1 884,146 - SGL_RS06015 0.70 -0.2 884,146 - SGL_RS06015 0.70 -0.1 884,146 - SGL_RS06015 0.70 +0.2 884,146 - SGL_RS06015 0.70 -1.0 884,146 - SGL_RS06015 0.70 -0.0 884,146 - SGL_RS06015 0.70 -3.9 884,146 - SGL_RS06015 0.70 +0.2 884,156 - SGL_RS06015 0.70 +0.2 884,167 - SGL_RS06015 0.71 -0.3 884,169 - SGL_RS06015 0.72 +0.3 884,209 + SGL_RS06015 0.75 -0.2 884,238 - SGL_RS06015 0.77 -0.2 884,240 + SGL_RS06015 0.77 +0.5 884,268 - SGL_RS06015 0.80 +0.1 884,280 - SGL_RS06015 0.81 +2.0 884,363 - SGL_RS06015 0.87 +0.1 884,398 - +0.3 884,500 - +0.3 884,504 + +0.1 884,512 - +0.1 884,512 - +0.1 884,671 - -3.6 884,672 - -3.5 884,971 + SGL_RS06020 0.37 -4.1 884,971 + SGL_RS06020 0.37 -3.0 884,979 - SGL_RS06020 0.38 -4.4 884,979 - SGL_RS06020 0.38 -4.3 884,979 - SGL_RS06020 0.38 -3.7 884,979 - SGL_RS06020 0.38 -4.3 884,979 - SGL_RS06020 0.38 -4.3 884,979 - SGL_RS06020 0.38 -3.7 884,979 - SGL_RS06020 0.38 -4.1 885,112 - SGL_RS06020 0.53 -3.0 885,159 - SGL_RS06020 0.58 -3.2 885,446 - -4.4 885,527 + +0.1 885,902 - SGL_RS06025 0.60 +0.2 886,264 + +1.7 886,280 + +1.9
Or see this region's nucleotide sequence