Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05435 and SGL_RS05440 are separated by 188 nucleotides SGL_RS05440 and SGL_RS05445 are separated by 281 nucleotides SGL_RS05445 and SGL_RS05450 are separated by 66 nucleotides
SGL_RS05435: SGL_RS05435 - heavy metal translocating P-type ATPase, at 764,301 to 766,538
_RS05435
SGL_RS05440: SGL_RS05440 - ACP S-malonyltransferase, at 766,727 to 767,608
_RS05440
SGL_RS05445: SGL_RS05445 - response regulator, at 767,890 to 768,432
_RS05445
SGL_RS05450: SGL_RS05450 - YbjN domain-containing protein, at 768,499 to 768,960
_RS05450
Position (kb) 766
767
768 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 766.018 kb on - strand, within SGL_RS05435 at 766.364 kb on - strand at 766.490 kb on + strand at 766.538 kb on + strand at 766.558 kb on + strand at 766.889 kb on - strand, within SGL_RS05440 at 767.218 kb on - strand, within SGL_RS05440 at 767.220 kb on - strand, within SGL_RS05440 at 767.223 kb on - strand, within SGL_RS05440 at 767.242 kb on + strand, within SGL_RS05440 at 767.320 kb on - strand, within SGL_RS05440 at 767.708 kb on + strand at 767.743 kb on - strand at 767.792 kb on + strand at 767.831 kb on + strand at 767.831 kb on - strand at 767.850 kb on - strand at 767.979 kb on + strand, within SGL_RS05445 at 768.005 kb on + strand, within SGL_RS05445 at 768.255 kb on - strand, within SGL_RS05445 at 768.525 kb on - strand at 768.565 kb on - strand, within SGL_RS05450
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 766,018 - SGL_RS05435 0.77 -0.7 766,364 - -0.4 766,490 + -0.8 766,538 + +0.2 766,558 + -0.1 766,889 - SGL_RS05440 0.18 -1.9 767,218 - SGL_RS05440 0.56 -2.7 767,220 - SGL_RS05440 0.56 -1.1 767,223 - SGL_RS05440 0.56 -3.1 767,242 + SGL_RS05440 0.58 -1.9 767,320 - SGL_RS05440 0.67 -4.4 767,708 + -0.1 767,743 - -0.4 767,792 + +0.0 767,831 + +0.0 767,831 - +0.9 767,850 - +0.9 767,979 + SGL_RS05445 0.16 +0.7 768,005 + SGL_RS05445 0.21 +1.5 768,255 - SGL_RS05445 0.67 +0.8 768,525 - +0.3 768,565 - SGL_RS05450 0.14 +0.1
Or see this region's nucleotide sequence