Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05320 and SGL_RS05325 are separated by 148 nucleotides SGL_RS05325 and SGL_RS05330 are separated by 91 nucleotides SGL_RS05330 and SGL_RS05335 are separated by 77 nucleotides SGL_RS05335 and SGL_RS05340 are separated by 274 nucleotides
SGL_RS05320: SGL_RS05320 - PBP1A family penicillin-binding protein, at 739,682 to 741,931
_RS05320
SGL_RS05325: SGL_RS05325 - PAP/fibrillin family protein, at 742,080 to 742,688
_RS05325
SGL_RS05330: SGL_RS05330 - ABC transporter ATP-binding protein, at 742,780 to 744,732
_RS05330
SGL_RS05335: SGL_RS05335 - hypothetical protein, at 744,810 to 745,064
_RS05335
SGL_RS05340: SGL_RS05340 - MBL fold metallo-hydrolase, at 745,339 to 746,124
_RS05340
Position (kb) 742
743
744
745 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 741.907 kb on - strand at 741.973 kb on - strand at 742.043 kb on - strand at 742.095 kb on + strand at 742.176 kb on - strand, within SGL_RS05325 at 742.217 kb on - strand, within SGL_RS05325 at 742.275 kb on + strand, within SGL_RS05325 at 742.397 kb on + strand, within SGL_RS05325 at 742.405 kb on - strand, within SGL_RS05325 at 742.464 kb on + strand, within SGL_RS05325 at 742.486 kb on + strand, within SGL_RS05325 at 742.554 kb on - strand, within SGL_RS05325 at 742.678 kb on + strand at 743.095 kb on + strand, within SGL_RS05330 at 743.150 kb on + strand, within SGL_RS05330 at 743.151 kb on + strand, within SGL_RS05330 at 743.273 kb on - strand, within SGL_RS05330 at 743.291 kb on - strand, within SGL_RS05330 at 743.680 kb on + strand, within SGL_RS05330 at 743.763 kb on + strand, within SGL_RS05330 at 743.763 kb on + strand, within SGL_RS05330 at 743.763 kb on + strand, within SGL_RS05330 at 743.771 kb on - strand, within SGL_RS05330 at 743.985 kb on + strand, within SGL_RS05330 at 744.053 kb on - strand, within SGL_RS05330 at 744.088 kb on + strand, within SGL_RS05330 at 744.173 kb on - strand, within SGL_RS05330 at 744.338 kb on + strand, within SGL_RS05330 at 744.359 kb on + strand, within SGL_RS05330 at 744.399 kb on - strand, within SGL_RS05330 at 744.400 kb on - strand, within SGL_RS05330 at 744.429 kb on + strand, within SGL_RS05330 at 744.732 kb on + strand at 744.897 kb on + strand, within SGL_RS05335 at 744.910 kb on - strand, within SGL_RS05335 at 744.915 kb on - strand, within SGL_RS05335 at 744.926 kb on - strand, within SGL_RS05335 at 744.945 kb on - strand, within SGL_RS05335 at 744.945 kb on - strand, within SGL_RS05335 at 744.950 kb on - strand, within SGL_RS05335 at 744.991 kb on + strand, within SGL_RS05335 at 745.027 kb on + strand, within SGL_RS05335 at 745.028 kb on - strand, within SGL_RS05335 at 745.034 kb on - strand, within SGL_RS05335 at 745.048 kb on + strand at 745.110 kb on - strand at 745.513 kb on + strand, within SGL_RS05340 at 745.571 kb on + strand, within SGL_RS05340
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 741,907 - -1.9 741,973 - -0.3 742,043 - +0.0 742,095 + -1.4 742,176 - SGL_RS05325 0.16 -1.0 742,217 - SGL_RS05325 0.22 -0.9 742,275 + SGL_RS05325 0.32 -1.0 742,397 + SGL_RS05325 0.52 -1.0 742,405 - SGL_RS05325 0.53 -0.3 742,464 + SGL_RS05325 0.63 -0.7 742,486 + SGL_RS05325 0.67 -1.0 742,554 - SGL_RS05325 0.78 -0.8 742,678 + -0.3 743,095 + SGL_RS05330 0.16 -6.7 743,150 + SGL_RS05330 0.19 -5.5 743,151 + SGL_RS05330 0.19 -6.4 743,273 - SGL_RS05330 0.25 -4.2 743,291 - SGL_RS05330 0.26 -6.4 743,680 + SGL_RS05330 0.46 -6.1 743,763 + SGL_RS05330 0.50 -2.1 743,763 + SGL_RS05330 0.50 -4.7 743,763 + SGL_RS05330 0.50 -5.1 743,771 - SGL_RS05330 0.51 -6.6 743,985 + SGL_RS05330 0.62 -5.4 744,053 - SGL_RS05330 0.65 -6.2 744,088 + SGL_RS05330 0.67 -4.7 744,173 - SGL_RS05330 0.71 -6.7 744,338 + SGL_RS05330 0.80 -6.4 744,359 + SGL_RS05330 0.81 -4.0 744,399 - SGL_RS05330 0.83 -5.2 744,400 - SGL_RS05330 0.83 -4.3 744,429 + SGL_RS05330 0.84 -3.4 744,732 + -4.2 744,897 + SGL_RS05335 0.34 -0.6 744,910 - SGL_RS05335 0.39 -0.3 744,915 - SGL_RS05335 0.41 -0.6 744,926 - SGL_RS05335 0.45 -0.4 744,945 - SGL_RS05335 0.53 +0.2 744,945 - SGL_RS05335 0.53 -0.5 744,950 - SGL_RS05335 0.55 -0.2 744,991 + SGL_RS05335 0.71 -0.3 745,027 + SGL_RS05335 0.85 -0.4 745,028 - SGL_RS05335 0.85 -0.1 745,034 - SGL_RS05335 0.88 -0.5 745,048 + -0.5 745,110 - -0.4 745,513 + SGL_RS05340 0.22 -0.4 745,571 + SGL_RS05340 0.30 -0.6
Or see this region's nucleotide sequence