Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05255 and SGL_RS05260 are separated by 170 nucleotides SGL_RS05260 and SGL_RS05265 are separated by 186 nucleotides SGL_RS05265 and SGL_RS05270 are separated by 236 nucleotides
SGL_RS05255: SGL_RS05255 - hypothetical protein, at 728,271 to 728,489
_RS05255
SGL_RS05260: SGL_RS05260 - hypothetical protein, at 728,660 to 729,484
_RS05260
SGL_RS05265: SGL_RS05265 - hypothetical protein, at 729,671 to 729,871
_RS05265
SGL_RS05270: SGL_RS05270 - serine/threonine-protein kinase, at 730,108 to 731,674
_RS05270
Position (kb) 728
729
730 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 727.847 kb on + strand at 728.248 kb on - strand at 728.531 kb on - strand at 728.847 kb on - strand, within SGL_RS05260 at 728.874 kb on - strand, within SGL_RS05260 at 728.990 kb on - strand, within SGL_RS05260 at 729.197 kb on - strand, within SGL_RS05260 at 729.291 kb on - strand, within SGL_RS05260 at 729.425 kb on + strand at 729.425 kb on + strand at 729.432 kb on + strand at 729.780 kb on - strand, within SGL_RS05265 at 729.800 kb on - strand, within SGL_RS05265 at 729.952 kb on + strand at 730.072 kb on + strand at 730.072 kb on + strand at 730.072 kb on + strand at 730.080 kb on - strand at 730.148 kb on - strand at 730.221 kb on + strand at 730.375 kb on - strand, within SGL_RS05270 at 730.402 kb on - strand, within SGL_RS05270
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 727,847 + -0.1 728,248 - +1.2 728,531 - -1.3 728,847 - SGL_RS05260 0.23 -3.3 728,874 - SGL_RS05260 0.26 -3.2 728,990 - SGL_RS05260 0.40 -3.0 729,197 - SGL_RS05260 0.65 -4.2 729,291 - SGL_RS05260 0.76 -2.1 729,425 + -0.9 729,425 + -1.1 729,432 + -0.9 729,780 - SGL_RS05265 0.54 +0.9 729,800 - SGL_RS05265 0.64 -0.2 729,952 + +0.1 730,072 + -0.2 730,072 + +0.3 730,072 + +0.5 730,080 - +0.4 730,148 - -0.6 730,221 + +2.1 730,375 - SGL_RS05270 0.17 +2.5 730,402 - SGL_RS05270 0.19 +2.3
Or see this region's nucleotide sequence