Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04780 and SGL_RS04785 are separated by 149 nucleotides SGL_RS04785 and SGL_RS04790 are separated by 52 nucleotides SGL_RS04790 and SGL_RS04795 are separated by 12 nucleotides SGL_RS04795 and SGL_RS04800 are separated by 304 nucleotides
SGL_RS04780: SGL_RS04780 - alpha/beta fold hydrolase, at 620,560 to 621,468
_RS04780
SGL_RS04785: SGL_RS04785 - CPP1-like family protein, at 621,618 to 622,247
_RS04785
SGL_RS04790: SGL_RS04790 - penicillin-binding protein 2, at 622,300 to 624,123
_RS04790
SGL_RS04795: SGL_RS04795 - hypothetical protein, at 624,136 to 624,594
_RS04795
SGL_RS04800: SGL_RS04800 - (Fe-S)-binding protein, at 624,899 to 626,281
_RS04800
Position (kb) 622
623
624
625 Strain fitness (log2 ratio) -2
-1
0
1 at 621.354 kb on + strand, within SGL_RS04780 at 621.362 kb on + strand, within SGL_RS04780 at 621.411 kb on - strand at 621.449 kb on + strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.457 kb on - strand at 621.462 kb on + strand at 621.462 kb on + strand at 621.481 kb on - strand at 621.483 kb on - strand at 621.531 kb on + strand at 621.531 kb on - strand at 621.608 kb on + strand at 621.711 kb on + strand, within SGL_RS04785 at 621.916 kb on - strand, within SGL_RS04785 at 622.013 kb on - strand, within SGL_RS04785 at 622.174 kb on + strand, within SGL_RS04785 at 622.308 kb on - strand at 622.317 kb on + strand at 624.257 kb on + strand, within SGL_RS04795 at 625.107 kb on + strand, within SGL_RS04800
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 621,354 + SGL_RS04780 0.87 -0.2 621,362 + SGL_RS04780 0.88 -0.1 621,411 - +0.0 621,449 + +0.0 621,457 - +0.1 621,457 - +0.2 621,457 - -0.5 621,462 + -0.1 621,462 + +0.4 621,481 - +0.6 621,483 - +0.1 621,531 + -1.5 621,531 - -0.0 621,608 + +1.3 621,711 + SGL_RS04785 0.15 +1.3 621,916 - SGL_RS04785 0.47 +0.7 622,013 - SGL_RS04785 0.63 +1.4 622,174 + SGL_RS04785 0.88 +0.5 622,308 - -0.9 622,317 + -1.9 624,257 + SGL_RS04795 0.26 -2.6 625,107 + SGL_RS04800 0.15 -0.8
Or see this region's nucleotide sequence