Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04250 and SGL_RS04255 are separated by 35 nucleotides SGL_RS04255 and SGL_RS04260 are separated by 88 nucleotides SGL_RS04260 and SGL_RS04265 are separated by 92 nucleotides
SGL_RS04250: SGL_RS04250 - enoyl-ACP reductase FabI, at 503,943 to 504,719
_RS04250
SGL_RS04255: SGL_RS04255 - tetratricopeptide repeat protein, at 504,755 to 505,267
_RS04255
SGL_RS04260: SGL_RS04260 - DUF2993 domain-containing protein, at 505,356 to 506,159
_RS04260
SGL_RS04265: SGL_RS04265 - calcium-binding protein, at 506,252 to 508,027
_RS04265
Position (kb) 505
506
507 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 504.439 kb on - strand, within SGL_RS04250 at 504.530 kb on - strand, within SGL_RS04250 at 504.816 kb on + strand, within SGL_RS04255 at 504.875 kb on + strand, within SGL_RS04255 at 504.899 kb on + strand, within SGL_RS04255 at 504.921 kb on - strand, within SGL_RS04255 at 504.946 kb on - strand, within SGL_RS04255 at 504.990 kb on - strand, within SGL_RS04255 at 505.007 kb on + strand, within SGL_RS04255 at 505.058 kb on - strand, within SGL_RS04255 at 505.132 kb on - strand, within SGL_RS04255 at 505.202 kb on + strand, within SGL_RS04255 at 505.217 kb on - strand at 505.343 kb on + strand at 505.435 kb on - strand at 505.507 kb on + strand, within SGL_RS04260 at 505.507 kb on + strand, within SGL_RS04260 at 505.515 kb on - strand, within SGL_RS04260 at 505.571 kb on - strand, within SGL_RS04260 at 505.665 kb on + strand, within SGL_RS04260 at 505.665 kb on + strand, within SGL_RS04260 at 505.665 kb on + strand, within SGL_RS04260 at 505.680 kb on + strand, within SGL_RS04260 at 505.698 kb on + strand, within SGL_RS04260 at 505.782 kb on - strand, within SGL_RS04260 at 505.786 kb on + strand, within SGL_RS04260 at 505.809 kb on - strand, within SGL_RS04260 at 505.886 kb on - strand, within SGL_RS04260 at 506.093 kb on + strand at 506.223 kb on + strand at 506.311 kb on - strand at 506.410 kb on - strand at 506.456 kb on - strand, within SGL_RS04265 at 506.458 kb on + strand, within SGL_RS04265 at 506.499 kb on - strand, within SGL_RS04265 at 506.815 kb on + strand, within SGL_RS04265 at 506.896 kb on - strand, within SGL_RS04265 at 506.910 kb on + strand, within SGL_RS04265 at 507.136 kb on - strand, within SGL_RS04265
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 504,439 - SGL_RS04250 0.64 -2.9 504,530 - SGL_RS04250 0.76 -1.5 504,816 + SGL_RS04255 0.12 -0.1 504,875 + SGL_RS04255 0.23 +0.1 504,899 + SGL_RS04255 0.28 -0.3 504,921 - SGL_RS04255 0.32 +0.2 504,946 - SGL_RS04255 0.37 -0.1 504,990 - SGL_RS04255 0.46 +0.4 505,007 + SGL_RS04255 0.49 +0.2 505,058 - SGL_RS04255 0.59 -0.0 505,132 - SGL_RS04255 0.73 +0.2 505,202 + SGL_RS04255 0.87 +0.3 505,217 - -0.8 505,343 + -0.3 505,435 - -4.9 505,507 + SGL_RS04260 0.19 -4.1 505,507 + SGL_RS04260 0.19 -4.7 505,515 - SGL_RS04260 0.20 -6.1 505,571 - SGL_RS04260 0.27 -2.5 505,665 + SGL_RS04260 0.38 -6.2 505,665 + SGL_RS04260 0.38 -5.7 505,665 + SGL_RS04260 0.38 -3.9 505,680 + SGL_RS04260 0.40 -5.7 505,698 + SGL_RS04260 0.43 -6.1 505,782 - SGL_RS04260 0.53 -4.7 505,786 + SGL_RS04260 0.53 -3.0 505,809 - SGL_RS04260 0.56 -4.5 505,886 - SGL_RS04260 0.66 -5.1 506,093 + -4.1 506,223 + -0.4 506,311 - +0.5 506,410 - +0.1 506,456 - SGL_RS04265 0.11 +0.2 506,458 + SGL_RS04265 0.12 +0.0 506,499 - SGL_RS04265 0.14 -0.1 506,815 + SGL_RS04265 0.32 -0.6 506,896 - SGL_RS04265 0.36 +0.4 506,910 + SGL_RS04265 0.37 -0.0 507,136 - SGL_RS04265 0.50 -0.3
Or see this region's nucleotide sequence