Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03575 and SGL_RS19770 are separated by 163 nucleotides SGL_RS19770 and SGL_RS03580 are separated by 4 nucleotides SGL_RS03580 and SGL_RS03585 are separated by 56 nucleotides SGL_RS03585 and SGL_RS03590 overlap by 4 nucleotides
SGL_RS03575: SGL_RS03575 - Uma2 family endonuclease, at 358,760 to 359,350
_RS03575
SGL_RS19770: SGL_RS19770 - DUF4160 domain-containing protein, at 359,514 to 359,663
_RS19770
SGL_RS03580: SGL_RS03580 - DUF2442 domain-containing protein, at 359,668 to 359,973
_RS03580
SGL_RS03585: SGL_RS03585 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 360,030 to 361,166
_RS03585
SGL_RS03590: SGL_RS03590 - ATP-grasp domain-containing protein, at 361,163 to 362,188
_RS03590
Position (kb) 360
361
362 Strain fitness (log2 ratio) -9
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 359.135 kb on - strand, within SGL_RS03575 at 359.477 kb on - strand at 360.007 kb on - strand at 360.074 kb on + strand at 360.203 kb on - strand, within SGL_RS03585 at 360.577 kb on + strand, within SGL_RS03585 at 360.585 kb on + strand, within SGL_RS03585 at 360.597 kb on + strand, within SGL_RS03585 at 360.630 kb on + strand, within SGL_RS03585 at 360.630 kb on + strand, within SGL_RS03585 at 360.653 kb on - strand, within SGL_RS03585 at 361.047 kb on - strand, within SGL_RS03585 at 361.603 kb on + strand, within SGL_RS03590 at 361.847 kb on + strand, within SGL_RS03590 at 361.889 kb on - strand, within SGL_RS03590 at 361.948 kb on + strand, within SGL_RS03590 at 362.055 kb on - strand, within SGL_RS03590
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 359,135 - SGL_RS03575 0.63 +0.3 359,477 - -0.1 360,007 - -0.4 360,074 + -6.6 360,203 - SGL_RS03585 0.15 -6.8 360,577 + SGL_RS03585 0.48 -8.1 360,585 + SGL_RS03585 0.49 -5.5 360,597 + SGL_RS03585 0.50 -5.8 360,630 + SGL_RS03585 0.53 -3.5 360,630 + SGL_RS03585 0.53 -6.2 360,653 - SGL_RS03585 0.55 -9.0 361,047 - SGL_RS03585 0.89 -6.5 361,603 + SGL_RS03590 0.43 -7.1 361,847 + SGL_RS03590 0.67 -6.7 361,889 - SGL_RS03590 0.71 -6.6 361,948 + SGL_RS03590 0.77 -5.8 362,055 - SGL_RS03590 0.87 -8.3
Or see this region's nucleotide sequence