Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03490 and SGL_RS03495 are separated by 23 nucleotides SGL_RS03495 and SGL_RS03500 are separated by 99 nucleotides SGL_RS03500 and SGL_RS03505 are separated by 65 nucleotides SGL_RS03505 and SGL_RS19420 are separated by 173 nucleotides SGL_RS19420 and SGL_RS03515 are separated by 577 nucleotides
SGL_RS03490: SGL_RS03490 - YaaW family protein, at 343,863 to 344,720
_RS03490
SGL_RS03495: SGL_RS03495 - metal-binding protein, at 344,744 to 345,298
_RS03495
SGL_RS03500: SGL_RS03500 - tRNA-Val, at 345,398 to 345,470
_RS03500
SGL_RS03505: SGL_RS03505 - exopolysaccharide transport family protein, at 345,536 to 347,806
_RS03505
SGL_RS19420: SGL_RS19420 - hypothetical protein, at 347,980 to 348,123
_RS19420
SGL_RS03515: SGL_RS03515 - Uma2 family endonuclease, at 348,701 to 349,429
_RS03515
Position (kb) 345
346
347
348 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 344.597 kb on - strand, within SGL_RS03490 at 344.613 kb on + strand, within SGL_RS03490 at 344.684 kb on - strand at 344.842 kb on + strand, within SGL_RS03495 at 344.850 kb on - strand, within SGL_RS03495 at 344.913 kb on - strand, within SGL_RS03495 at 344.965 kb on + strand, within SGL_RS03495 at 345.178 kb on + strand, within SGL_RS03495 at 345.265 kb on + strand at 345.305 kb on + strand at 345.605 kb on + strand at 345.605 kb on - strand at 345.882 kb on - strand, within SGL_RS03505 at 345.977 kb on + strand, within SGL_RS03505 at 346.174 kb on + strand, within SGL_RS03505 at 346.205 kb on + strand, within SGL_RS03505 at 346.231 kb on - strand, within SGL_RS03505 at 346.272 kb on - strand, within SGL_RS03505 at 346.276 kb on + strand, within SGL_RS03505 at 346.326 kb on + strand, within SGL_RS03505 at 346.361 kb on + strand, within SGL_RS03505 at 346.379 kb on - strand, within SGL_RS03505 at 346.504 kb on + strand, within SGL_RS03505 at 346.512 kb on - strand, within SGL_RS03505 at 346.512 kb on - strand, within SGL_RS03505 at 346.534 kb on + strand, within SGL_RS03505 at 346.663 kb on + strand, within SGL_RS03505 at 346.737 kb on + strand, within SGL_RS03505 at 346.745 kb on - strand, within SGL_RS03505 at 346.821 kb on + strand, within SGL_RS03505 at 346.974 kb on - strand, within SGL_RS03505 at 347.103 kb on - strand, within SGL_RS03505 at 347.336 kb on + strand, within SGL_RS03505 at 347.360 kb on - strand, within SGL_RS03505 at 347.668 kb on + strand at 347.690 kb on + strand at 347.753 kb on + strand at 347.804 kb on - strand at 347.858 kb on + strand at 347.883 kb on + strand at 347.931 kb on - strand at 347.933 kb on + strand at 347.933 kb on + strand at 347.933 kb on + strand at 347.985 kb on - strand at 347.999 kb on - strand, within SGL_RS19420 at 348.102 kb on - strand, within SGL_RS19420 at 348.139 kb on + strand at 348.395 kb on - strand at 348.396 kb on + strand at 348.456 kb on + strand at 348.595 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 344,597 - SGL_RS03490 0.86 +2.5 344,613 + SGL_RS03490 0.87 +2.6 344,684 - +2.6 344,842 + SGL_RS03495 0.18 -1.1 344,850 - SGL_RS03495 0.19 -0.7 344,913 - SGL_RS03495 0.30 -0.7 344,965 + SGL_RS03495 0.40 -1.2 345,178 + SGL_RS03495 0.78 -0.9 345,265 + +0.4 345,305 + -1.0 345,605 + +0.1 345,605 - +0.1 345,882 - SGL_RS03505 0.15 -3.2 345,977 + SGL_RS03505 0.19 -5.1 346,174 + SGL_RS03505 0.28 -3.6 346,205 + SGL_RS03505 0.29 -4.8 346,231 - SGL_RS03505 0.31 -4.7 346,272 - SGL_RS03505 0.32 -4.0 346,276 + SGL_RS03505 0.33 -5.3 346,326 + SGL_RS03505 0.35 -5.7 346,361 + SGL_RS03505 0.36 -3.8 346,379 - SGL_RS03505 0.37 -2.9 346,504 + SGL_RS03505 0.43 -5.1 346,512 - SGL_RS03505 0.43 -3.5 346,512 - SGL_RS03505 0.43 -5.3 346,534 + SGL_RS03505 0.44 -5.2 346,663 + SGL_RS03505 0.50 -3.0 346,737 + SGL_RS03505 0.53 -6.1 346,745 - SGL_RS03505 0.53 -4.2 346,821 + SGL_RS03505 0.57 -4.3 346,974 - SGL_RS03505 0.63 -3.4 347,103 - SGL_RS03505 0.69 -6.2 347,336 + SGL_RS03505 0.79 -4.0 347,360 - SGL_RS03505 0.80 -5.6 347,668 + -5.0 347,690 + -4.4 347,753 + -4.7 347,804 - -5.3 347,858 + -1.3 347,883 + -0.1 347,931 - -0.3 347,933 + +0.3 347,933 + +0.2 347,933 + -0.4 347,985 - +0.1 347,999 - SGL_RS19420 0.13 -0.2 348,102 - SGL_RS19420 0.85 -0.2 348,139 + -0.6 348,395 - +0.1 348,396 + -0.2 348,456 + +0.3 348,595 + +0.0
Or see this region's nucleotide sequence