Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03275 and SGL_RS03280 are separated by 226 nucleotides SGL_RS03280 and SGL_RS03285 are separated by 12 nucleotides SGL_RS03285 and SGL_RS03290 overlap by 4 nucleotides
SGL_RS03275: SGL_RS03275 - UDP-glucose/GDP-mannose dehydrogenase family protein, at 299,522 to 300,883
_RS03275
SGL_RS03280: SGL_RS03280 - FAD-dependent hydroxylase, at 301,110 to 302,354
_RS03280
SGL_RS03285: SGL_RS03285 - fluoride efflux transporter CrcB, at 302,367 to 302,789
_RS03285
SGL_RS03290: SGL_RS03290 - LapA family protein, at 302,786 to 303,190
_RS03290
Position (kb) 301
302
303 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 300.199 kb on + strand, within SGL_RS03275 at 300.281 kb on - strand, within SGL_RS03275 at 300.399 kb on - strand, within SGL_RS03275 at 300.407 kb on + strand, within SGL_RS03275 at 300.414 kb on - strand, within SGL_RS03275 at 300.581 kb on - strand, within SGL_RS03275 at 300.583 kb on + strand, within SGL_RS03275 at 300.710 kb on + strand, within SGL_RS03275 at 300.781 kb on - strand at 300.783 kb on + strand at 300.791 kb on - strand at 300.869 kb on - strand at 300.893 kb on - strand at 300.941 kb on - strand at 300.963 kb on - strand at 301.045 kb on + strand at 301.115 kb on - strand at 302.354 kb on + strand at 302.410 kb on - strand, within SGL_RS03285 at 302.440 kb on - strand, within SGL_RS03285 at 302.459 kb on - strand, within SGL_RS03285 at 302.489 kb on + strand, within SGL_RS03285 at 302.497 kb on - strand, within SGL_RS03285 at 302.511 kb on - strand, within SGL_RS03285 at 302.550 kb on + strand, within SGL_RS03285 at 302.686 kb on - strand, within SGL_RS03285 at 302.973 kb on - strand, within SGL_RS03290 at 303.064 kb on - strand, within SGL_RS03290 at 303.142 kb on + strand, within SGL_RS03290 at 303.344 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 300,199 + SGL_RS03275 0.50 -5.4 300,281 - SGL_RS03275 0.56 -3.3 300,399 - SGL_RS03275 0.64 -3.9 300,407 + SGL_RS03275 0.65 -3.4 300,414 - SGL_RS03275 0.65 -4.8 300,581 - SGL_RS03275 0.78 -2.5 300,583 + SGL_RS03275 0.78 -3.6 300,710 + SGL_RS03275 0.87 -4.3 300,781 - -3.7 300,783 + -4.1 300,791 - -3.3 300,869 - -4.5 300,893 - +0.3 300,941 - +0.2 300,963 - +0.5 301,045 + -0.2 301,115 - +0.4 302,354 + -0.5 302,410 - SGL_RS03285 0.10 +0.5 302,440 - SGL_RS03285 0.17 +0.2 302,459 - SGL_RS03285 0.22 +0.1 302,489 + SGL_RS03285 0.29 +0.1 302,497 - SGL_RS03285 0.31 -0.1 302,511 - SGL_RS03285 0.34 +0.1 302,550 + SGL_RS03285 0.43 +0.2 302,686 - SGL_RS03285 0.75 +0.5 302,973 - SGL_RS03290 0.46 -3.0 303,064 - SGL_RS03290 0.69 -0.6 303,142 + SGL_RS03290 0.88 +0.3 303,344 - -2.3
Or see this region's nucleotide sequence