Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03245 and SGL_RS03250 are separated by 81 nucleotides SGL_RS03250 and SGL_RS19555 are separated by 10 nucleotides SGL_RS19555 and SGL_RS03255 are separated by 208 nucleotides
SGL_RS03245: SGL_RS03245 - Fe(3+) ABC transporter substrate-binding protein, at 291,626 to 292,708
_RS03245
SGL_RS03250: SGL_RS03250 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 292,790 to 293,485
_RS03250
SGL_RS19555: SGL_RS19555 - hypothetical protein, at 293,496 to 293,669
_RS19555
SGL_RS03255: SGL_RS03255 - ATP-dependent 6-phosphofructokinase, at 293,878 to 294,963
_RS03255
Position (kb) 292
293
294 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 291.820 kb on - strand, within SGL_RS03245 at 291.891 kb on - strand, within SGL_RS03245 at 291.898 kb on + strand, within SGL_RS03245 at 291.898 kb on + strand, within SGL_RS03245 at 292.224 kb on + strand, within SGL_RS03245 at 292.230 kb on + strand, within SGL_RS03245 at 292.242 kb on - strand, within SGL_RS03245 at 292.248 kb on - strand, within SGL_RS03245 at 292.282 kb on - strand, within SGL_RS03245 at 292.307 kb on + strand, within SGL_RS03245 at 292.358 kb on - strand, within SGL_RS03245 at 292.371 kb on + strand, within SGL_RS03245 at 292.447 kb on + strand, within SGL_RS03245 at 292.471 kb on + strand, within SGL_RS03245 at 292.487 kb on + strand, within SGL_RS03245 at 292.489 kb on - strand, within SGL_RS03245 at 292.509 kb on + strand, within SGL_RS03245 at 292.659 kb on - strand at 292.661 kb on + strand at 292.669 kb on - strand at 292.669 kb on - strand at 292.781 kb on + strand at 292.895 kb on - strand, within SGL_RS03250 at 292.923 kb on + strand, within SGL_RS03250 at 293.154 kb on + strand, within SGL_RS03250 at 293.167 kb on - strand, within SGL_RS03250 at 293.227 kb on - strand, within SGL_RS03250 at 293.227 kb on - strand, within SGL_RS03250 at 293.330 kb on - strand, within SGL_RS03250 at 293.412 kb on + strand, within SGL_RS03250 at 293.585 kb on - strand, within SGL_RS19555 at 293.607 kb on - strand, within SGL_RS19555 at 293.698 kb on + strand at 293.859 kb on + strand at 293.986 kb on + strand at 294.065 kb on + strand, within SGL_RS03255 at 294.065 kb on + strand, within SGL_RS03255 at 294.087 kb on - strand, within SGL_RS03255 at 294.123 kb on - strand, within SGL_RS03255 at 294.226 kb on - strand, within SGL_RS03255 at 294.232 kb on + strand, within SGL_RS03255 at 294.245 kb on - strand, within SGL_RS03255 at 294.261 kb on + strand, within SGL_RS03255 at 294.318 kb on + strand, within SGL_RS03255
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 291,820 - SGL_RS03245 0.18 +2.5 291,891 - SGL_RS03245 0.24 +2.8 291,898 + SGL_RS03245 0.25 +2.5 291,898 + SGL_RS03245 0.25 +2.6 292,224 + SGL_RS03245 0.55 +3.1 292,230 + SGL_RS03245 0.56 +2.5 292,242 - SGL_RS03245 0.57 +3.2 292,248 - SGL_RS03245 0.57 +2.6 292,282 - SGL_RS03245 0.61 +2.7 292,307 + SGL_RS03245 0.63 +3.4 292,358 - SGL_RS03245 0.68 +2.8 292,371 + SGL_RS03245 0.69 +2.1 292,447 + SGL_RS03245 0.76 +3.2 292,471 + SGL_RS03245 0.78 +2.9 292,487 + SGL_RS03245 0.80 +2.9 292,489 - SGL_RS03245 0.80 +2.5 292,509 + SGL_RS03245 0.82 +3.2 292,659 - +2.4 292,661 + +2.8 292,669 - +2.8 292,669 - +2.6 292,781 + +0.9 292,895 - SGL_RS03250 0.15 -5.3 292,923 + SGL_RS03250 0.19 -3.2 293,154 + SGL_RS03250 0.52 -1.8 293,167 - SGL_RS03250 0.54 -4.6 293,227 - SGL_RS03250 0.63 -2.7 293,227 - SGL_RS03250 0.63 -4.2 293,330 - SGL_RS03250 0.78 -1.9 293,412 + SGL_RS03250 0.89 -4.6 293,585 - SGL_RS19555 0.51 +0.3 293,607 - SGL_RS19555 0.64 -0.1 293,698 + +0.1 293,859 + +0.5 293,986 + +0.0 294,065 + SGL_RS03255 0.17 -0.3 294,065 + SGL_RS03255 0.17 -0.1 294,087 - SGL_RS03255 0.19 +0.8 294,123 - SGL_RS03255 0.23 +0.2 294,226 - SGL_RS03255 0.32 +0.2 294,232 + SGL_RS03255 0.33 -0.1 294,245 - SGL_RS03255 0.34 -0.2 294,261 + SGL_RS03255 0.35 -0.0 294,318 + SGL_RS03255 0.41 +0.6
Or see this region's nucleotide sequence