Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02995 and SGL_RS03000 are separated by 15 nucleotides SGL_RS03000 and SGL_RS03005 are separated by 74 nucleotides SGL_RS03005 and SGL_RS03010 are separated by 107 nucleotides
SGL_RS02995: SGL_RS02995 - Gfo/Idh/MocA family oxidoreductase, at 243,158 to 244,144
_RS02995
SGL_RS03000: SGL_RS03000 - spore maturation protein, at 244,160 to 244,708
_RS03000
SGL_RS03005: SGL_RS03005 - nucleoside recognition domain-containing protein, at 244,783 to 245,394
_RS03005
SGL_RS03010: SGL_RS03010 - 4-alpha-glucanotransferase, at 245,502 to 247,019
_RS03010
Position (kb) 244
245 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 243.505 kb on - strand, within SGL_RS02995 at 243.709 kb on - strand, within SGL_RS02995 at 243.738 kb on + strand, within SGL_RS02995 at 243.741 kb on + strand, within SGL_RS02995 at 244.181 kb on + strand at 244.408 kb on + strand, within SGL_RS03000 at 244.417 kb on - strand, within SGL_RS03000 at 244.461 kb on + strand, within SGL_RS03000 at 244.540 kb on - strand, within SGL_RS03000 at 244.570 kb on - strand, within SGL_RS03000 at 245.163 kb on + strand, within SGL_RS03005 at 245.171 kb on - strand, within SGL_RS03005 at 245.406 kb on - strand at 245.426 kb on - strand at 245.468 kb on - strand at 245.601 kb on - strand at 245.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 243,505 - SGL_RS02995 0.35 +2.1 243,709 - SGL_RS02995 0.56 +0.7 243,738 + SGL_RS02995 0.59 -0.2 243,741 + SGL_RS02995 0.59 -0.5 244,181 + -6.0 244,408 + SGL_RS03000 0.45 -5.3 244,417 - SGL_RS03000 0.47 -6.4 244,461 + SGL_RS03000 0.55 -6.2 244,540 - SGL_RS03000 0.69 -0.5 244,570 - SGL_RS03000 0.75 -5.9 245,163 + SGL_RS03005 0.62 -6.6 245,171 - SGL_RS03005 0.63 -6.4 245,406 - -6.7 245,426 - -5.0 245,468 - +0.3 245,601 - +3.2 245,620 - +2.4
Or see this region's nucleotide sequence