Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02655 and SGL_RS02660 are separated by 12 nucleotides SGL_RS02660 and SGL_RS02665 are separated by 5 nucleotides SGL_RS02665 and SGL_RS02670 are separated by 98 nucleotides
SGL_RS02655: SGL_RS02655 - S41 family peptidase, at 168,533 to 169,921
_RS02655
SGL_RS02660: SGL_RS02660 - L-threonylcarbamoyladenylate synthase, at 169,934 to 170,566
_RS02660
SGL_RS02665: SGL_RS02665 - nickel pincer cofactor biosynthesis protein LarC, at 170,572 to 171,840
_RS02665
SGL_RS02670: SGL_RS02670 - F0F1 ATP synthase subunit gamma, at 171,939 to 172,883
_RS02670
Position (kb)
170
171
172 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 169.585 kb on + strand, within SGL_RS02655 at 169.761 kb on + strand, within SGL_RS02655 at 169.969 kb on - strand at 170.164 kb on - strand, within SGL_RS02660 at 170.351 kb on - strand, within SGL_RS02660 at 170.377 kb on + strand, within SGL_RS02660 at 170.458 kb on - strand, within SGL_RS02660 at 170.482 kb on - strand, within SGL_RS02660 at 171.136 kb on + strand, within SGL_RS02665 at 171.514 kb on - strand, within SGL_RS02665 at 171.520 kb on + strand, within SGL_RS02665
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 169,585 + SGL_RS02655 0.76 -2.3 169,761 + SGL_RS02655 0.88 -1.7 169,969 - +0.5 170,164 - SGL_RS02660 0.36 +0.3 170,351 - SGL_RS02660 0.66 +0.9 170,377 + SGL_RS02660 0.70 +0.7 170,458 - SGL_RS02660 0.83 +0.9 170,482 - SGL_RS02660 0.87 +0.5 171,136 + SGL_RS02665 0.44 -2.8 171,514 - SGL_RS02665 0.74 -3.9 171,520 + SGL_RS02665 0.75 -2.6
Or see this region's nucleotide sequence