Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01980 and SGL_RS01985 are separated by 67 nucleotides SGL_RS01985 and SGL_RS01990 are separated by 72 nucleotides SGL_RS01990 and SGL_RS01995 are separated by 69 nucleotides SGL_RS01995 and SGL_RS02000 overlap by 17 nucleotides
SGL_RS01980: SGL_RS01980 - AraC family transcriptional regulator, at 27,990 to 29,030
_RS01980
SGL_RS01985: SGL_RS01985 - class I SAM-dependent methyltransferase, at 29,098 to 29,895
_RS01985
SGL_RS01990: SGL_RS01990 - TonB-dependent siderophore receptor, at 29,968 to 32,454
_RS01990
SGL_RS01995: SGL_RS01995 - biopolymer transporter ExbD, at 32,524 to 32,928
_RS01995
SGL_RS02000: SGL_RS02000 - MotA/TolQ/ExbB proton channel family protein, at 32,912 to 33,544
_RS02000
Position (kb) 29
30
31
32
33 Strain fitness (log2 ratio) -1
0
1
2
3 at 29.294 kb on + strand, within SGL_RS01985 at 29.507 kb on + strand, within SGL_RS01985 at 29.746 kb on + strand, within SGL_RS01985 at 29.951 kb on - strand at 29.964 kb on - strand at 30.023 kb on - strand at 30.047 kb on + strand at 30.202 kb on + strand at 30.550 kb on + strand, within SGL_RS01990 at 30.550 kb on + strand, within SGL_RS01990 at 30.603 kb on + strand, within SGL_RS01990 at 30.719 kb on + strand, within SGL_RS01990 at 30.832 kb on + strand, within SGL_RS01990 at 31.293 kb on + strand, within SGL_RS01990 at 31.313 kb on + strand, within SGL_RS01990 at 31.313 kb on + strand, within SGL_RS01990 at 31.345 kb on + strand, within SGL_RS01990 at 31.386 kb on + strand, within SGL_RS01990 at 31.638 kb on + strand, within SGL_RS01990 at 31.786 kb on + strand, within SGL_RS01990 at 31.786 kb on - strand, within SGL_RS01990 at 31.787 kb on - strand, within SGL_RS01990 at 31.796 kb on + strand, within SGL_RS01990 at 31.851 kb on - strand, within SGL_RS01990 at 31.873 kb on - strand, within SGL_RS01990 at 31.879 kb on - strand, within SGL_RS01990 at 31.899 kb on - strand, within SGL_RS01990 at 31.939 kb on + strand, within SGL_RS01990 at 31.993 kb on + strand, within SGL_RS01990 at 32.013 kb on + strand, within SGL_RS01990 at 32.013 kb on + strand, within SGL_RS01990 at 32.182 kb on + strand, within SGL_RS01990 at 32.182 kb on + strand, within SGL_RS01990 at 32.202 kb on + strand, within SGL_RS01990 at 32.210 kb on - strand at 32.228 kb on + strand at 32.233 kb on + strand at 32.236 kb on - strand at 32.277 kb on + strand at 32.292 kb on - strand at 32.386 kb on + strand at 32.419 kb on + strand at 32.431 kb on + strand at 32.571 kb on + strand, within SGL_RS01995 at 32.704 kb on - strand, within SGL_RS01995 at 32.903 kb on + strand at 32.911 kb on - strand at 33.016 kb on + strand, within SGL_RS02000 at 33.039 kb on + strand, within SGL_RS02000 at 33.044 kb on + strand, within SGL_RS02000 at 33.071 kb on + strand, within SGL_RS02000 at 33.071 kb on + strand, within SGL_RS02000 at 33.086 kb on + strand, within SGL_RS02000 at 33.151 kb on - strand, within SGL_RS02000 at 33.361 kb on - strand, within SGL_RS02000
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 29,294 + SGL_RS01985 0.25 +0.3 29,507 + SGL_RS01985 0.51 -0.6 29,746 + SGL_RS01985 0.81 +0.5 29,951 - +1.0 29,964 - +0.8 30,023 - +0.9 30,047 + +0.6 30,202 + +1.2 30,550 + SGL_RS01990 0.23 +2.4 30,550 + SGL_RS01990 0.23 +1.6 30,603 + SGL_RS01990 0.26 +1.8 30,719 + SGL_RS01990 0.30 +1.8 30,832 + SGL_RS01990 0.35 +1.5 31,293 + SGL_RS01990 0.53 +1.9 31,313 + SGL_RS01990 0.54 +1.8 31,313 + SGL_RS01990 0.54 +2.1 31,345 + SGL_RS01990 0.55 +2.0 31,386 + SGL_RS01990 0.57 +1.3 31,638 + SGL_RS01990 0.67 +1.7 31,786 + SGL_RS01990 0.73 +1.6 31,786 - SGL_RS01990 0.73 +1.8 31,787 - SGL_RS01990 0.73 +3.2 31,796 + SGL_RS01990 0.74 +2.0 31,851 - SGL_RS01990 0.76 +1.4 31,873 - SGL_RS01990 0.77 +1.6 31,879 - SGL_RS01990 0.77 +1.7 31,899 - SGL_RS01990 0.78 +1.6 31,939 + SGL_RS01990 0.79 +2.2 31,993 + SGL_RS01990 0.81 +1.8 32,013 + SGL_RS01990 0.82 +1.7 32,013 + SGL_RS01990 0.82 +0.8 32,182 + SGL_RS01990 0.89 +2.1 32,182 + SGL_RS01990 0.89 +2.3 32,202 + SGL_RS01990 0.90 +1.8 32,210 - +2.2 32,228 + +2.1 32,233 + +1.9 32,236 - +1.9 32,277 + +1.8 32,292 - +1.4 32,386 + +2.1 32,419 + +2.1 32,431 + +0.8 32,571 + SGL_RS01995 0.12 +1.6 32,704 - SGL_RS01995 0.44 +1.5 32,903 + +0.6 32,911 - +1.1 33,016 + SGL_RS02000 0.16 +1.5 33,039 + SGL_RS02000 0.20 +1.4 33,044 + SGL_RS02000 0.21 +1.3 33,071 + SGL_RS02000 0.25 +1.3 33,071 + SGL_RS02000 0.25 +1.5 33,086 + SGL_RS02000 0.27 +1.0 33,151 - SGL_RS02000 0.38 +1.1 33,361 - SGL_RS02000 0.71 +2.1
Or see this region's nucleotide sequence