Experiment: Nickel (II) sulfate 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01260 and SGL_RS01265 overlap by 4 nucleotides SGL_RS01265 and SGL_RS01270 are separated by 217 nucleotides SGL_RS01270 and SGL_RS01275 are separated by 285 nucleotides
SGL_RS01260: SGL_RS01260 - putative toxin-antitoxin system toxin component, PIN family, at 23,434 to 23,859
_RS01260
SGL_RS01265: SGL_RS01265 - AbrB/MazE/SpoVT family DNA-binding domain-containing protein, at 23,856 to 24,077
_RS01265
SGL_RS01270: SGL_RS01270 - recombinase family protein, at 24,295 to 24,861
_RS01270
SGL_RS01275: SGL_RS01275 - DUF839 domain-containing protein, at 25,147 to 27,345
_RS01275
Position (kb)
24
25 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 23.774 kb on - strand, within SGL_RS01260 at 23.774 kb on - strand, within SGL_RS01260 at 23.774 kb on - strand, within SGL_RS01260 at 23.774 kb on - strand, within SGL_RS01260 at 23.823 kb on + strand at 23.854 kb on + strand at 23.860 kb on - strand at 23.902 kb on + strand, within SGL_RS01265 at 23.907 kb on + strand, within SGL_RS01265 at 23.910 kb on - strand, within SGL_RS01265 at 23.910 kb on - strand, within SGL_RS01265 at 23.910 kb on - strand, within SGL_RS01265 at 23.962 kb on - strand, within SGL_RS01265 at 23.974 kb on + strand, within SGL_RS01265 at 23.979 kb on + strand, within SGL_RS01265 at 23.987 kb on - strand, within SGL_RS01265 at 24.000 kb on + strand, within SGL_RS01265 at 24.064 kb on + strand at 24.177 kb on + strand at 24.200 kb on - strand at 24.316 kb on - strand at 24.318 kb on + strand at 24.326 kb on - strand at 24.359 kb on + strand, within SGL_RS01270 at 24.411 kb on + strand, within SGL_RS01270 at 24.421 kb on + strand, within SGL_RS01270 at 24.432 kb on - strand, within SGL_RS01270 at 24.444 kb on + strand, within SGL_RS01270 at 24.526 kb on + strand, within SGL_RS01270 at 24.611 kb on - strand, within SGL_RS01270 at 24.620 kb on - strand, within SGL_RS01270 at 24.752 kb on + strand, within SGL_RS01270 at 24.752 kb on + strand, within SGL_RS01270 at 24.937 kb on + strand at 24.944 kb on + strand at 25.007 kb on + strand at 25.150 kb on - strand at 25.209 kb on - strand at 25.214 kb on - strand at 25.410 kb on - strand, within SGL_RS01275 at 25.478 kb on - strand, within SGL_RS01275 at 25.592 kb on + strand, within SGL_RS01275 at 25.682 kb on - strand, within SGL_RS01275 at 25.720 kb on + strand, within SGL_RS01275 at 25.728 kb on + strand, within SGL_RS01275 at 25.737 kb on - strand, within SGL_RS01275
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) sulfate 3 uM remove 23,774 - SGL_RS01260 0.80 +0.1 23,774 - SGL_RS01260 0.80 -0.5 23,774 - SGL_RS01260 0.80 +0.6 23,774 - SGL_RS01260 0.80 -1.3 23,823 + -0.8 23,854 + -0.1 23,860 - -0.1 23,902 + SGL_RS01265 0.21 +0.9 23,907 + SGL_RS01265 0.23 +0.3 23,910 - SGL_RS01265 0.24 +0.1 23,910 - SGL_RS01265 0.24 -0.1 23,910 - SGL_RS01265 0.24 -0.6 23,962 - SGL_RS01265 0.48 +0.3 23,974 + SGL_RS01265 0.53 +0.9 23,979 + SGL_RS01265 0.55 -0.9 23,987 - SGL_RS01265 0.59 -0.1 24,000 + SGL_RS01265 0.65 +0.5 24,064 + -0.5 24,177 + -0.3 24,200 - -1.7 24,316 - -0.6 24,318 + -0.7 24,326 - -0.9 24,359 + SGL_RS01270 0.11 -2.5 24,411 + SGL_RS01270 0.20 -1.1 24,421 + SGL_RS01270 0.22 -1.0 24,432 - SGL_RS01270 0.24 -0.4 24,444 + SGL_RS01270 0.26 -0.6 24,526 + SGL_RS01270 0.41 +0.9 24,611 - SGL_RS01270 0.56 -1.2 24,620 - SGL_RS01270 0.57 -0.8 24,752 + SGL_RS01270 0.81 -0.6 24,752 + SGL_RS01270 0.81 -0.5 24,937 + -0.7 24,944 + +0.2 25,007 + +1.0 25,150 - +1.2 25,209 - +0.0 25,214 - -0.1 25,410 - SGL_RS01275 0.12 +0.6 25,478 - SGL_RS01275 0.15 -3.3 25,592 + SGL_RS01275 0.20 -0.3 25,682 - SGL_RS01275 0.24 +0.6 25,720 + SGL_RS01275 0.26 +0.1 25,728 + SGL_RS01275 0.26 +0.7 25,737 - SGL_RS01275 0.27 +0.9
Or see this region's nucleotide sequence