Experiment: Bathocuproine disulfonic 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05865 and SGL_RS05870 are separated by 87 nucleotides SGL_RS05870 and SGL_RS05875 are separated by 91 nucleotides SGL_RS05875 and SGL_RS05880 are separated by 180 nucleotides
SGL_RS05865: SGL_RS05865 - metallophosphoesterase, at 844,228 to 845,394
_RS05865
SGL_RS05870: SGL_RS05870 - DUF1823 family protein, at 845,482 to 845,874
_RS05870
SGL_RS05875: SGL_RS05875 - c-type cytochrome, at 845,966 to 846,328
_RS05875
SGL_RS05880: SGL_RS05880 - sugar ABC transporter substrate-binding protein, at 846,509 to 847,810
_RS05880
Position (kb) 845
846
847 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 844.995 kb on + strand, within SGL_RS05865 at 845.003 kb on - strand, within SGL_RS05865 at 845.046 kb on - strand, within SGL_RS05865 at 845.212 kb on + strand, within SGL_RS05865 at 845.212 kb on + strand, within SGL_RS05865 at 845.335 kb on - strand at 845.374 kb on + strand at 845.571 kb on - strand, within SGL_RS05870 at 845.669 kb on - strand, within SGL_RS05870 at 845.758 kb on + strand, within SGL_RS05870 at 845.914 kb on + strand at 846.154 kb on + strand, within SGL_RS05875 at 846.369 kb on - strand at 846.391 kb on - strand at 846.779 kb on - strand, within SGL_RS05880 at 846.815 kb on - strand, within SGL_RS05880 at 846.876 kb on + strand, within SGL_RS05880 at 846.876 kb on - strand, within SGL_RS05880 at 847.184 kb on + strand, within SGL_RS05880 at 847.301 kb on - strand, within SGL_RS05880
Per-strain Table
Position Strand Gene LocusTag Fraction Bathocuproine disulfonic 0.3 mM remove 844,995 + SGL_RS05865 0.66 -0.6 845,003 - SGL_RS05865 0.66 -0.2 845,046 - SGL_RS05865 0.70 +0.4 845,212 + SGL_RS05865 0.84 +0.4 845,212 + SGL_RS05865 0.84 +0.3 845,335 - -0.3 845,374 + -0.2 845,571 - SGL_RS05870 0.23 +0.0 845,669 - SGL_RS05870 0.48 +0.3 845,758 + SGL_RS05870 0.70 -0.0 845,914 + -0.0 846,154 + SGL_RS05875 0.52 -3.4 846,369 - -0.8 846,391 - -0.9 846,779 - SGL_RS05880 0.21 -1.6 846,815 - SGL_RS05880 0.24 +1.7 846,876 + SGL_RS05880 0.28 -3.5 846,876 - SGL_RS05880 0.28 -3.2 847,184 + SGL_RS05880 0.52 -3.3 847,301 - SGL_RS05880 0.61 -2.0
Or see this region's nucleotide sequence