Experiment: Bathocuproine disulfonic 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03280 and SGL_RS03285 are separated by 12 nucleotides SGL_RS03285 and SGL_RS03290 overlap by 4 nucleotides SGL_RS03290 and SGL_RS03295 are separated by 233 nucleotides
SGL_RS03280: SGL_RS03280 - FAD-dependent hydroxylase, at 301,110 to 302,354
_RS03280
SGL_RS03285: SGL_RS03285 - fluoride efflux transporter CrcB, at 302,367 to 302,789
_RS03285
SGL_RS03290: SGL_RS03290 - LapA family protein, at 302,786 to 303,190
_RS03290
SGL_RS03295: SGL_RS03295 - hypothetical protein, at 303,424 to 303,621
_RS03295
Position (kb) 302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 302.354 kb on + strand at 302.410 kb on - strand, within SGL_RS03285 at 302.440 kb on - strand, within SGL_RS03285 at 302.459 kb on - strand, within SGL_RS03285 at 302.489 kb on + strand, within SGL_RS03285 at 302.497 kb on - strand, within SGL_RS03285 at 302.511 kb on - strand, within SGL_RS03285 at 302.550 kb on + strand, within SGL_RS03285 at 302.686 kb on - strand, within SGL_RS03285 at 302.973 kb on - strand, within SGL_RS03290 at 303.064 kb on - strand, within SGL_RS03290 at 303.142 kb on + strand, within SGL_RS03290 at 303.344 kb on - strand at 303.403 kb on - strand at 303.648 kb on - strand at 303.710 kb on - strand at 303.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bathocuproine disulfonic 0.3 mM remove 302,354 + -0.4 302,410 - SGL_RS03285 0.10 +1.0 302,440 - SGL_RS03285 0.17 +0.1 302,459 - SGL_RS03285 0.22 -0.0 302,489 + SGL_RS03285 0.29 -0.0 302,497 - SGL_RS03285 0.31 +0.1 302,511 - SGL_RS03285 0.34 +0.5 302,550 + SGL_RS03285 0.43 +0.1 302,686 - SGL_RS03285 0.75 +0.3 302,973 - SGL_RS03290 0.46 -3.3 303,064 - SGL_RS03290 0.69 -0.1 303,142 + SGL_RS03290 0.88 +0.1 303,344 - -1.7 303,403 - +0.0 303,648 - -0.0 303,710 - -0.2 303,732 - -0.0
Or see this region's nucleotide sequence