Experiment: Copper (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01990 and SGL_RS01995 are separated by 69 nucleotides SGL_RS01995 and SGL_RS02000 overlap by 17 nucleotides SGL_RS02000 and SGL_RS02005 are separated by 346 nucleotides
SGL_RS01990: SGL_RS01990 - TonB-dependent siderophore receptor, at 29,968 to 32,454
_RS01990
SGL_RS01995: SGL_RS01995 - biopolymer transporter ExbD, at 32,524 to 32,928
_RS01995
SGL_RS02000: SGL_RS02000 - MotA/TolQ/ExbB proton channel family protein, at 32,912 to 33,544
_RS02000
SGL_RS02005: SGL_RS02005 - energy transducer TonB, at 33,891 to 35,489
_RS02005
Position (kb) 32
33
34 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 31.939 kb on + strand, within SGL_RS01990 at 31.993 kb on + strand, within SGL_RS01990 at 32.013 kb on + strand, within SGL_RS01990 at 32.013 kb on + strand, within SGL_RS01990 at 32.182 kb on + strand, within SGL_RS01990 at 32.182 kb on + strand, within SGL_RS01990 at 32.202 kb on + strand, within SGL_RS01990 at 32.210 kb on - strand at 32.228 kb on + strand at 32.233 kb on + strand at 32.236 kb on - strand at 32.277 kb on + strand at 32.292 kb on - strand at 32.386 kb on + strand at 32.419 kb on + strand at 32.431 kb on + strand at 32.571 kb on + strand, within SGL_RS01995 at 32.704 kb on - strand, within SGL_RS01995 at 32.903 kb on + strand at 32.911 kb on - strand at 33.016 kb on + strand, within SGL_RS02000 at 33.039 kb on + strand, within SGL_RS02000 at 33.044 kb on + strand, within SGL_RS02000 at 33.071 kb on + strand, within SGL_RS02000 at 33.071 kb on + strand, within SGL_RS02000 at 33.086 kb on + strand, within SGL_RS02000 at 33.151 kb on - strand, within SGL_RS02000 at 33.361 kb on - strand, within SGL_RS02000 at 33.571 kb on + strand at 33.574 kb on - strand at 33.969 kb on + strand at 34.012 kb on - strand at 34.014 kb on - strand at 34.047 kb on - strand at 34.072 kb on + strand, within SGL_RS02005 at 34.089 kb on - strand, within SGL_RS02005 at 34.151 kb on + strand, within SGL_RS02005 at 34.194 kb on + strand, within SGL_RS02005 at 34.199 kb on + strand, within SGL_RS02005 at 34.229 kb on + strand, within SGL_RS02005 at 34.229 kb on + strand, within SGL_RS02005 at 34.236 kb on + strand, within SGL_RS02005 at 34.277 kb on - strand, within SGL_RS02005 at 34.310 kb on + strand, within SGL_RS02005 at 34.318 kb on - strand, within SGL_RS02005 at 34.318 kb on - strand, within SGL_RS02005 at 34.348 kb on - strand, within SGL_RS02005 at 34.372 kb on - strand, within SGL_RS02005 at 34.372 kb on - strand, within SGL_RS02005 at 34.376 kb on + strand, within SGL_RS02005 at 34.384 kb on - strand, within SGL_RS02005 at 34.394 kb on - strand, within SGL_RS02005 at 34.394 kb on - strand, within SGL_RS02005 at 34.531 kb on - strand, within SGL_RS02005
Per-strain Table
Position Strand Gene LocusTag Fraction Copper (II) sulfate 5 uM remove 31,939 + SGL_RS01990 0.79 -1.4 31,993 + SGL_RS01990 0.81 -1.7 32,013 + SGL_RS01990 0.82 -1.1 32,013 + SGL_RS01990 0.82 +0.1 32,182 + SGL_RS01990 0.89 -1.1 32,182 + SGL_RS01990 0.89 -0.8 32,202 + SGL_RS01990 0.90 -1.1 32,210 - -0.9 32,228 + -1.3 32,233 + -1.9 32,236 - -1.4 32,277 + -2.2 32,292 - -1.4 32,386 + -1.3 32,419 + -1.1 32,431 + -1.6 32,571 + SGL_RS01995 0.12 -2.4 32,704 - SGL_RS01995 0.44 -2.2 32,903 + -3.5 32,911 - -1.0 33,016 + SGL_RS02000 0.16 -2.1 33,039 + SGL_RS02000 0.20 -2.2 33,044 + SGL_RS02000 0.21 -2.0 33,071 + SGL_RS02000 0.25 -1.9 33,071 + SGL_RS02000 0.25 -1.5 33,086 + SGL_RS02000 0.27 -2.8 33,151 - SGL_RS02000 0.38 -2.3 33,361 - SGL_RS02000 0.71 -1.3 33,571 + -0.5 33,574 - -2.4 33,969 + -0.9 34,012 - -0.9 34,014 - -0.8 34,047 - -0.9 34,072 + SGL_RS02005 0.11 -0.8 34,089 - SGL_RS02005 0.12 -0.2 34,151 + SGL_RS02005 0.16 -0.1 34,194 + SGL_RS02005 0.19 -0.6 34,199 + SGL_RS02005 0.19 -0.5 34,229 + SGL_RS02005 0.21 -0.8 34,229 + SGL_RS02005 0.21 -0.5 34,236 + SGL_RS02005 0.22 -0.4 34,277 - SGL_RS02005 0.24 -1.2 34,310 + SGL_RS02005 0.26 -1.0 34,318 - SGL_RS02005 0.27 -0.9 34,318 - SGL_RS02005 0.27 -0.4 34,348 - SGL_RS02005 0.29 -0.8 34,372 - SGL_RS02005 0.30 -0.1 34,372 - SGL_RS02005 0.30 -0.8 34,376 + SGL_RS02005 0.30 +1.0 34,384 - SGL_RS02005 0.31 -0.7 34,394 - SGL_RS02005 0.31 -0.2 34,394 - SGL_RS02005 0.31 -0.7 34,531 - SGL_RS02005 0.40 -0.5
Or see this region's nucleotide sequence