Experiment: Copper (II) sulfate 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01140 and SGL_RS01145 overlap by 4 nucleotides SGL_RS01145 and SGL_RS01150 overlap by 8 nucleotides SGL_RS01150 and SGL_RS01155 overlap by 4 nucleotides SGL_RS01155 and SGL_RS01160 overlap by 4 nucleotides SGL_RS01160 and SGL_RS01165 overlap by 17 nucleotides SGL_RS01165 and SGL_RS01170 are separated by 50 nucleotides
SGL_RS01140: SGL_RS01140 - hypothetical protein, at 3,660 to 3,839
_RS01140
SGL_RS01145: SGL_RS01145 - zeta toxin family protein, at 3,836 to 4,372
_RS01145
SGL_RS01150: SGL_RS01150 - nucleotidyltransferase family protein, at 4,365 to 4,655
_RS01150
SGL_RS01155: SGL_RS01155 - restriction endonuclease subunit S, at 4,652 to 6,046
_RS01155
SGL_RS01160: SGL_RS01160 - DUF5615 family PIN-like protein, at 6,043 to 6,393
_RS01160
SGL_RS01165: SGL_RS01165 - DUF433 domain-containing protein, at 6,377 to 6,601
_RS01165
SGL_RS01170: SGL_RS01170 - class I SAM-dependent DNA methyltransferase, at 6,652 to 8,619
_RS01170
Position (kb) 4
5
6
7 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3.862 kb on + strand at 3.884 kb on + strand at 4.015 kb on + strand, within SGL_RS01145 at 4.035 kb on - strand, within SGL_RS01145 at 4.070 kb on + strand, within SGL_RS01145 at 4.114 kb on + strand, within SGL_RS01145 at 4.132 kb on + strand, within SGL_RS01145 at 4.139 kb on + strand, within SGL_RS01145 at 4.140 kb on - strand, within SGL_RS01145 at 4.161 kb on - strand, within SGL_RS01145 at 4.266 kb on - strand, within SGL_RS01145 at 4.312 kb on - strand, within SGL_RS01145 at 4.534 kb on + strand, within SGL_RS01150 at 4.572 kb on - strand, within SGL_RS01150 at 4.624 kb on - strand, within SGL_RS01150 at 4.650 kb on - strand at 4.659 kb on + strand at 5.140 kb on - strand, within SGL_RS01155 at 5.365 kb on + strand, within SGL_RS01155 at 5.611 kb on + strand, within SGL_RS01155 at 5.611 kb on + strand, within SGL_RS01155 at 5.612 kb on + strand, within SGL_RS01155 at 5.652 kb on - strand, within SGL_RS01155 at 6.015 kb on - strand at 6.027 kb on + strand at 6.064 kb on + strand at 6.115 kb on - strand, within SGL_RS01160 at 6.209 kb on - strand, within SGL_RS01160 at 6.368 kb on - strand at 6.375 kb on + strand at 6.739 kb on + strand at 6.816 kb on + strand at 6.829 kb on + strand at 6.829 kb on + strand at 6.837 kb on - strand at 6.837 kb on - strand at 6.924 kb on + strand, within SGL_RS01170 at 6.924 kb on + strand, within SGL_RS01170 at 6.930 kb on - strand, within SGL_RS01170 at 6.937 kb on - strand, within SGL_RS01170 at 6.942 kb on - strand, within SGL_RS01170 at 6.954 kb on - strand, within SGL_RS01170 at 6.977 kb on - strand, within SGL_RS01170
Per-strain Table
Position Strand Gene LocusTag Fraction Copper (II) sulfate 5 uM remove 3,862 + -5.3 3,884 + -0.2 4,015 + SGL_RS01145 0.33 +0.2 4,035 - SGL_RS01145 0.37 +0.3 4,070 + SGL_RS01145 0.44 -3.0 4,114 + SGL_RS01145 0.52 -0.4 4,132 + SGL_RS01145 0.55 -0.2 4,139 + SGL_RS01145 0.56 +1.7 4,140 - SGL_RS01145 0.57 -0.3 4,161 - SGL_RS01145 0.61 +0.1 4,266 - SGL_RS01145 0.80 +0.5 4,312 - SGL_RS01145 0.89 -0.4 4,534 + SGL_RS01150 0.58 -0.2 4,572 - SGL_RS01150 0.71 +1.0 4,624 - SGL_RS01150 0.89 -2.5 4,650 - +0.5 4,659 + +1.1 5,140 - SGL_RS01155 0.35 -1.0 5,365 + SGL_RS01155 0.51 -0.0 5,611 + SGL_RS01155 0.69 -0.3 5,611 + SGL_RS01155 0.69 +0.7 5,612 + SGL_RS01155 0.69 -0.2 5,652 - SGL_RS01155 0.72 +1.6 6,015 - -0.4 6,027 + -0.2 6,064 + -0.0 6,115 - SGL_RS01160 0.21 +0.1 6,209 - SGL_RS01160 0.47 +0.6 6,368 - -0.2 6,375 + -0.5 6,739 + +0.4 6,816 + -0.4 6,829 + +0.7 6,829 + +0.6 6,837 - -0.7 6,837 - -0.4 6,924 + SGL_RS01170 0.14 -1.7 6,924 + SGL_RS01170 0.14 +0.1 6,930 - SGL_RS01170 0.14 +0.3 6,937 - SGL_RS01170 0.14 -0.4 6,942 - SGL_RS01170 0.15 -0.5 6,954 - SGL_RS01170 0.15 -1.4 6,977 - SGL_RS01170 0.17 +0.2
Or see this region's nucleotide sequence