Experiment: no stress control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01895 and SGL_RS01900 are separated by 182 nucleotides SGL_RS01900 and SGL_RS01905 are separated by 144 nucleotides
SGL_RS01895: SGL_RS01895 - magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, at 3,192 to 4,268
_RS01895
SGL_RS01900: SGL_RS01900 - GDP-L-fucose synthase, at 4,451 to 5,389
_RS01900
SGL_RS01905: SGL_RS01905 - GDP-mannose 4,6-dehydratase, at 5,534 to 6,622
_RS01905
Position (kb) 4
5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4.628 kb on + strand, within SGL_RS01900 at 4.682 kb on - strand, within SGL_RS01900 at 4.878 kb on - strand, within SGL_RS01900 at 4.916 kb on + strand, within SGL_RS01900 at 5.037 kb on - strand, within SGL_RS01900 at 5.178 kb on - strand, within SGL_RS01900 at 5.178 kb on - strand, within SGL_RS01900 at 5.198 kb on + strand, within SGL_RS01900 at 5.237 kb on + strand, within SGL_RS01900 at 5.571 kb on + strand at 5.760 kb on - strand, within SGL_RS01905 at 5.873 kb on + strand, within SGL_RS01905 at 5.917 kb on + strand, within SGL_RS01905 at 5.918 kb on - strand, within SGL_RS01905 at 5.936 kb on - strand, within SGL_RS01905 at 6.026 kb on + strand, within SGL_RS01905 at 6.026 kb on + strand, within SGL_RS01905 at 6.119 kb on + strand, within SGL_RS01905 at 6.132 kb on + strand, within SGL_RS01905 at 6.137 kb on + strand, within SGL_RS01905 at 6.145 kb on - strand, within SGL_RS01905 at 6.145 kb on - strand, within SGL_RS01905 at 6.246 kb on + strand, within SGL_RS01905 at 6.355 kb on - strand, within SGL_RS01905
Per-strain Table
Position Strand Gene LocusTag Fraction no stress control remove 4,628 + SGL_RS01900 0.19 -1.8 4,682 - SGL_RS01900 0.25 -2.3 4,878 - SGL_RS01900 0.45 -0.5 4,916 + SGL_RS01900 0.50 -1.5 5,037 - SGL_RS01900 0.62 -0.8 5,178 - SGL_RS01900 0.77 -1.3 5,178 - SGL_RS01900 0.77 -2.2 5,198 + SGL_RS01900 0.80 -1.9 5,237 + SGL_RS01900 0.84 -1.1 5,571 + -1.8 5,760 - SGL_RS01905 0.21 -4.4 5,873 + SGL_RS01905 0.31 -2.1 5,917 + SGL_RS01905 0.35 -2.7 5,918 - SGL_RS01905 0.35 -1.4 5,936 - SGL_RS01905 0.37 -2.4 6,026 + SGL_RS01905 0.45 -2.2 6,026 + SGL_RS01905 0.45 -3.2 6,119 + SGL_RS01905 0.54 -2.6 6,132 + SGL_RS01905 0.55 -3.2 6,137 + SGL_RS01905 0.55 -3.7 6,145 - SGL_RS01905 0.56 -3.0 6,145 - SGL_RS01905 0.56 -2.6 6,246 + SGL_RS01905 0.65 -2.7 6,355 - SGL_RS01905 0.75 -2.2
Or see this region's nucleotide sequence