Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17510 and SGL_RS17515 are separated by 42 nucleotides SGL_RS17515 and SGL_RS17520 are separated by 283 nucleotides SGL_RS17520 and SGL_RS17530 are separated by 228 nucleotides SGL_RS17530 and SGL_RS17535 are separated by 68 nucleotides
SGL_RS17510: SGL_RS17510 - hypothetical protein, at 3,385,642 to 3,385,962
_RS17510
SGL_RS17515: SGL_RS17515 - SMC-Scp complex subunit ScpB, at 3,386,005 to 3,386,493
_RS17515
SGL_RS17520: SGL_RS17520 - DUF3352 domain-containing protein, at 3,386,777 to 3,388,465
_RS17520
SGL_RS17530: SGL_RS17530 - DUF2231 domain-containing protein, at 3,388,694 to 3,389,326
_RS17530
SGL_RS17535: SGL_RS17535 - DUF2231 domain-containing protein, at 3,389,395 to 3,389,883
_RS17535
Position (kb)
3386
3387
3388
3389 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3385.821 kb on + strand, within SGL_RS17510 at 3385.917 kb on + strand, within SGL_RS17510 at 3386.029 kb on - strand at 3386.091 kb on + strand, within SGL_RS17515 at 3386.091 kb on + strand, within SGL_RS17515 at 3386.110 kb on - strand, within SGL_RS17515 at 3386.133 kb on + strand, within SGL_RS17515 at 3386.267 kb on - strand, within SGL_RS17515 at 3386.743 kb on + strand at 3387.227 kb on - strand, within SGL_RS17520 at 3387.257 kb on - strand, within SGL_RS17520 at 3387.355 kb on - strand, within SGL_RS17520 at 3387.370 kb on + strand, within SGL_RS17520 at 3387.448 kb on + strand, within SGL_RS17520 at 3387.636 kb on - strand, within SGL_RS17520 at 3387.637 kb on + strand, within SGL_RS17520 at 3387.871 kb on - strand, within SGL_RS17520 at 3387.930 kb on + strand, within SGL_RS17520 at 3388.065 kb on + strand, within SGL_RS17520 at 3388.089 kb on + strand, within SGL_RS17520 at 3388.100 kb on - strand, within SGL_RS17520 at 3388.119 kb on - strand, within SGL_RS17520 at 3388.504 kb on + strand at 3388.504 kb on + strand at 3388.528 kb on + strand at 3388.528 kb on + strand at 3388.528 kb on + strand at 3388.536 kb on - strand at 3388.598 kb on + strand at 3388.649 kb on + strand at 3388.667 kb on - strand at 3388.759 kb on - strand, within SGL_RS17530 at 3388.761 kb on + strand, within SGL_RS17530 at 3388.790 kb on + strand, within SGL_RS17530 at 3388.800 kb on - strand, within SGL_RS17530 at 3389.002 kb on - strand, within SGL_RS17530 at 3389.091 kb on + strand, within SGL_RS17530 at 3389.096 kb on + strand, within SGL_RS17530 at 3389.099 kb on - strand, within SGL_RS17530 at 3389.104 kb on - strand, within SGL_RS17530 at 3389.266 kb on + strand at 3389.274 kb on - strand at 3389.328 kb on - strand at 3389.400 kb on + strand at 3389.436 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 3,385,821 + SGL_RS17510 0.56 +0.1 3,385,917 + SGL_RS17510 0.86 +0.2 3,386,029 - -0.4 3,386,091 + SGL_RS17515 0.18 -0.6 3,386,091 + SGL_RS17515 0.18 -0.8 3,386,110 - SGL_RS17515 0.21 -0.5 3,386,133 + SGL_RS17515 0.26 -2.5 3,386,267 - SGL_RS17515 0.54 -0.2 3,386,743 + -0.3 3,387,227 - SGL_RS17520 0.27 -1.9 3,387,257 - SGL_RS17520 0.28 -2.2 3,387,355 - SGL_RS17520 0.34 -2.4 3,387,370 + SGL_RS17520 0.35 -0.5 3,387,448 + SGL_RS17520 0.40 -0.5 3,387,636 - SGL_RS17520 0.51 -2.6 3,387,637 + SGL_RS17520 0.51 -3.1 3,387,871 - SGL_RS17520 0.65 -4.3 3,387,930 + SGL_RS17520 0.68 -4.1 3,388,065 + SGL_RS17520 0.76 -1.5 3,388,089 + SGL_RS17520 0.78 -2.7 3,388,100 - SGL_RS17520 0.78 -1.2 3,388,119 - SGL_RS17520 0.79 -1.6 3,388,504 + -0.3 3,388,504 + -0.6 3,388,528 + +0.3 3,388,528 + +0.5 3,388,528 + -1.3 3,388,536 - +0.1 3,388,598 + -0.6 3,388,649 + -1.2 3,388,667 - -0.0 3,388,759 - SGL_RS17530 0.10 -0.6 3,388,761 + SGL_RS17530 0.11 +0.2 3,388,790 + SGL_RS17530 0.15 +0.4 3,388,800 - SGL_RS17530 0.17 +0.3 3,389,002 - SGL_RS17530 0.49 -0.2 3,389,091 + SGL_RS17530 0.63 +0.2 3,389,096 + SGL_RS17530 0.64 -0.3 3,389,099 - SGL_RS17530 0.64 -0.3 3,389,104 - SGL_RS17530 0.65 -0.4 3,389,266 + +0.1 3,389,274 - +0.1 3,389,328 - -0.3 3,389,400 + -0.6 3,389,436 + +0.2
Or see this region's nucleotide sequence