Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16870 and SGL_RS16875 are separated by 106 nucleotides SGL_RS16875 and SGL_RS16880 are separated by 4 nucleotides SGL_RS16880 and SGL_RS20195 are separated by 136 nucleotides SGL_RS20195 and SGL_RS16890 are separated by 164 nucleotides
SGL_RS16870: SGL_RS16870 - molecular chaperone DnaJ, at 3,237,125 to 3,238,258
_RS16870
SGL_RS16875: SGL_RS16875 - crossover junction endodeoxyribonuclease RuvC, at 3,238,365 to 3,238,868
_RS16875
SGL_RS16880: SGL_RS16880 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 3,238,873 to 3,239,748
_RS16880
SGL_RS20195: SGL_RS20195 - hypothetical protein, at 3,239,885 to 3,240,016
_RS20195
SGL_RS16890: SGL_RS16890 - indole-3-glycerol phosphate synthase TrpC, at 3,240,181 to 3,241,068
_RS16890
Position (kb) 3238
3239
3240 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3238.982 kb on + strand, within SGL_RS16880 at 3239.280 kb on - strand, within SGL_RS16880 at 3239.346 kb on - strand, within SGL_RS16880 at 3239.476 kb on - strand, within SGL_RS16880 at 3239.667 kb on - strand at 3239.815 kb on - strand at 3239.853 kb on - strand at 3239.858 kb on + strand at 3240.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 3,238,982 + SGL_RS16880 0.12 -1.6 3,239,280 - SGL_RS16880 0.46 -4.1 3,239,346 - SGL_RS16880 0.54 -3.0 3,239,476 - SGL_RS16880 0.69 -4.9 3,239,667 - -3.1 3,239,815 - -0.1 3,239,853 - +0.4 3,239,858 + -0.2 3,240,063 - +0.3
Or see this region's nucleotide sequence