Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16625 and SGL_RS16630 are separated by 128 nucleotides SGL_RS16630 and SGL_RS20180 are separated by 61 nucleotides SGL_RS20180 and SGL_RS16640 are separated by 255 nucleotides
SGL_RS16625: SGL_RS16625 - UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2, 6-diaminopimelate ligase, at 3,184,733 to 3,186,250
_RS16625
SGL_RS16630: SGL_RS16630 - MBL fold metallo-hydrolase, at 3,186,379 to 3,188,043
_RS16630
SGL_RS20180: SGL_RS20180 - membrane protein, at 3,188,105 to 3,188,227
_RS20180
SGL_RS16640: SGL_RS16640 - ABC transporter substrate-binding protein, at 3,188,483 to 3,189,748
_RS16640
Position (kb)
3186
3187
3188
3189 Strain fitness (log2 ratio)
-2
-1
0
1 at 3186.323 kb on + strand at 3186.429 kb on + strand at 3186.429 kb on + strand at 3186.437 kb on - strand at 3186.437 kb on - strand at 3186.531 kb on + strand at 3186.576 kb on + strand, within SGL_RS16630 at 3186.674 kb on + strand, within SGL_RS16630 at 3186.710 kb on - strand, within SGL_RS16630 at 3186.741 kb on + strand, within SGL_RS16630 at 3186.746 kb on + strand, within SGL_RS16630 at 3186.754 kb on - strand, within SGL_RS16630 at 3186.778 kb on - strand, within SGL_RS16630 at 3186.885 kb on - strand, within SGL_RS16630 at 3186.903 kb on + strand, within SGL_RS16630 at 3187.026 kb on + strand, within SGL_RS16630 at 3187.028 kb on + strand, within SGL_RS16630 at 3187.154 kb on + strand, within SGL_RS16630 at 3187.328 kb on - strand, within SGL_RS16630 at 3187.355 kb on + strand, within SGL_RS16630 at 3187.363 kb on + strand, within SGL_RS16630 at 3187.385 kb on + strand, within SGL_RS16630 at 3187.399 kb on + strand, within SGL_RS16630 at 3187.399 kb on + strand, within SGL_RS16630 at 3187.598 kb on - strand, within SGL_RS16630 at 3187.902 kb on + strand at 3187.915 kb on + strand at 3187.933 kb on - strand at 3187.947 kb on + strand at 3187.952 kb on + strand at 3187.977 kb on - strand at 3188.062 kb on + strand at 3188.202 kb on - strand, within SGL_RS20180 at 3188.279 kb on + strand at 3188.320 kb on - strand at 3188.544 kb on + strand at 3188.544 kb on + strand at 3188.552 kb on - strand at 3188.552 kb on - strand at 3188.557 kb on - strand at 3188.602 kb on - strand at 3188.633 kb on + strand, within SGL_RS16640 at 3188.638 kb on - strand, within SGL_RS16640 at 3188.658 kb on + strand, within SGL_RS16640 at 3188.721 kb on - strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.836 kb on + strand, within SGL_RS16640 at 3188.889 kb on + strand, within SGL_RS16640 at 3189.005 kb on - strand, within SGL_RS16640
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 3,186,323 + +0.1 3,186,429 + -0.8 3,186,429 + -1.3 3,186,437 - -0.3 3,186,437 - -0.9 3,186,531 + -1.1 3,186,576 + SGL_RS16630 0.12 -2.2 3,186,674 + SGL_RS16630 0.18 -1.2 3,186,710 - SGL_RS16630 0.20 -1.2 3,186,741 + SGL_RS16630 0.22 -1.2 3,186,746 + SGL_RS16630 0.22 -0.7 3,186,754 - SGL_RS16630 0.23 -1.6 3,186,778 - SGL_RS16630 0.24 -1.5 3,186,885 - SGL_RS16630 0.30 -0.8 3,186,903 + SGL_RS16630 0.31 -0.8 3,187,026 + SGL_RS16630 0.39 -1.0 3,187,028 + SGL_RS16630 0.39 -1.0 3,187,154 + SGL_RS16630 0.47 -1.6 3,187,328 - SGL_RS16630 0.57 -0.6 3,187,355 + SGL_RS16630 0.59 -0.7 3,187,363 + SGL_RS16630 0.59 -0.0 3,187,385 + SGL_RS16630 0.60 -1.7 3,187,399 + SGL_RS16630 0.61 -1.4 3,187,399 + SGL_RS16630 0.61 -1.0 3,187,598 - SGL_RS16630 0.73 -1.3 3,187,902 + -1.4 3,187,915 + -1.2 3,187,933 - -2.0 3,187,947 + -0.3 3,187,952 + -1.4 3,187,977 - -2.7 3,188,062 + -0.2 3,188,202 - SGL_RS20180 0.79 -0.3 3,188,279 + +0.2 3,188,320 - -0.0 3,188,544 + +0.2 3,188,544 + +0.2 3,188,552 - +0.8 3,188,552 - +0.2 3,188,557 - -0.0 3,188,602 - -0.0 3,188,633 + SGL_RS16640 0.12 -0.0 3,188,638 - SGL_RS16640 0.12 -0.1 3,188,658 + SGL_RS16640 0.14 -0.4 3,188,721 - SGL_RS16640 0.19 -0.2 3,188,836 + SGL_RS16640 0.28 +0.2 3,188,836 + SGL_RS16640 0.28 +0.0 3,188,836 + SGL_RS16640 0.28 +0.2 3,188,889 + SGL_RS16640 0.32 +0.1 3,189,005 - SGL_RS16640 0.41 +0.6
Or see this region's nucleotide sequence