Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15320 and SGL_RS15325 are separated by 142 nucleotides SGL_RS15325 and SGL_RS15330 are separated by 245 nucleotides SGL_RS15330 and SGL_RS15335 are separated by 2 nucleotides
SGL_RS15320: SGL_RS15320 - DEAD/DEAH box helicase, at 2,887,644 to 2,889,122
_RS15320
SGL_RS15325: SGL_RS15325 - N-acetyl-gamma-glutamyl-phosphate reductase, at 2,889,265 to 2,890,320
_RS15325
SGL_RS15330: SGL_RS15330 - imidazole glycerol phosphate synthase subunit HisH, at 2,890,566 to 2,891,198
_RS15330
SGL_RS15335: SGL_RS15335 - threonine--tRNA ligase, at 2,891,201 to 2,893,012
_RS15335
Position (kb)
2889
2890
2891 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2888.266 kb on + strand, within SGL_RS15320 at 2888.276 kb on + strand, within SGL_RS15320 at 2888.599 kb on + strand, within SGL_RS15320 at 2888.682 kb on + strand, within SGL_RS15320 at 2888.706 kb on + strand, within SGL_RS15320 at 2888.831 kb on - strand, within SGL_RS15320 at 2888.897 kb on - strand, within SGL_RS15320 at 2889.062 kb on + strand at 2889.203 kb on - strand at 2889.389 kb on - strand, within SGL_RS15325 at 2889.616 kb on + strand, within SGL_RS15325 at 2890.085 kb on + strand, within SGL_RS15325 at 2890.119 kb on - strand, within SGL_RS15325 at 2890.469 kb on + strand at 2890.542 kb on + strand at 2890.550 kb on - strand at 2890.689 kb on - strand, within SGL_RS15330
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,888,266 + SGL_RS15320 0.42 +0.5 2,888,276 + SGL_RS15320 0.43 +0.9 2,888,599 + SGL_RS15320 0.65 +0.3 2,888,682 + SGL_RS15320 0.70 +0.8 2,888,706 + SGL_RS15320 0.72 +0.6 2,888,831 - SGL_RS15320 0.80 -0.0 2,888,897 - SGL_RS15320 0.85 +1.6 2,889,062 + +0.4 2,889,203 - -0.0 2,889,389 - SGL_RS15325 0.12 -2.7 2,889,616 + SGL_RS15325 0.33 -2.2 2,890,085 + SGL_RS15325 0.78 -0.9 2,890,119 - SGL_RS15325 0.81 -2.5 2,890,469 + -0.0 2,890,542 + -1.3 2,890,550 - -2.2 2,890,689 - SGL_RS15330 0.19 -3.1
Or see this region's nucleotide sequence