Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14860 and SGL_RS14865 are separated by 157 nucleotides SGL_RS14865 and SGL_RS14870 are separated by 5 nucleotides SGL_RS14870 and SGL_RS14875 are separated by 49 nucleotides
SGL_RS14860: SGL_RS14860 - 8-amino-7-oxononanoate synthase, at 2,791,820 to 2,793,001
_RS14860
SGL_RS14865: SGL_RS14865 - type I methionyl aminopeptidase, at 2,793,159 to 2,793,920
_RS14865
SGL_RS14870: SGL_RS14870 - thiol-disulfide oxidoreductase DCC family protein, at 2,793,926 to 2,794,381
_RS14870
SGL_RS14875: SGL_RS14875 - NUDIX hydrolase, at 2,794,431 to 2,794,880
_RS14875
Position (kb)
2793
2794 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2792.228 kb on - strand, within SGL_RS14860 at 2792.501 kb on - strand, within SGL_RS14860 at 2792.542 kb on + strand, within SGL_RS14860 at 2792.555 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.643 kb on + strand, within SGL_RS14860 at 2792.806 kb on + strand, within SGL_RS14860 at 2792.932 kb on + strand at 2792.946 kb on + strand at 2793.026 kb on - strand at 2793.065 kb on + strand at 2793.177 kb on - strand at 2793.231 kb on + strand at 2793.481 kb on - strand, within SGL_RS14865 at 2793.850 kb on - strand at 2794.007 kb on + strand, within SGL_RS14870 at 2794.061 kb on + strand, within SGL_RS14870 at 2794.069 kb on - strand, within SGL_RS14870 at 2794.069 kb on - strand, within SGL_RS14870 at 2794.121 kb on + strand, within SGL_RS14870 at 2794.415 kb on + strand at 2794.468 kb on - strand at 2794.532 kb on + strand, within SGL_RS14875 at 2794.581 kb on - strand, within SGL_RS14875 at 2794.640 kb on + strand, within SGL_RS14875 at 2794.789 kb on + strand, within SGL_RS14875
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,792,228 - SGL_RS14860 0.35 -3.5 2,792,501 - SGL_RS14860 0.58 -3.0 2,792,542 + SGL_RS14860 0.61 -0.4 2,792,555 - SGL_RS14860 0.62 -2.7 2,792,560 - SGL_RS14860 0.63 -3.2 2,792,560 - SGL_RS14860 0.63 -1.3 2,792,560 - SGL_RS14860 0.63 -3.3 2,792,643 + SGL_RS14860 0.70 -1.7 2,792,806 + SGL_RS14860 0.83 -2.3 2,792,932 + -2.3 2,792,946 + -3.0 2,793,026 - -0.0 2,793,065 + -0.5 2,793,177 - -1.1 2,793,231 + -0.4 2,793,481 - SGL_RS14865 0.42 -1.2 2,793,850 - -0.7 2,794,007 + SGL_RS14870 0.18 +0.7 2,794,061 + SGL_RS14870 0.30 +0.2 2,794,069 - SGL_RS14870 0.31 +0.3 2,794,069 - SGL_RS14870 0.31 -0.5 2,794,121 + SGL_RS14870 0.43 +0.1 2,794,415 + -0.1 2,794,468 - +0.5 2,794,532 + SGL_RS14875 0.22 -0.0 2,794,581 - SGL_RS14875 0.33 +0.3 2,794,640 + SGL_RS14875 0.46 +0.1 2,794,789 + SGL_RS14875 0.80 +0.2
Or see this region's nucleotide sequence