Experiment: Ethanol 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14855 and SGL_RS14860 are separated by 293 nucleotides SGL_RS14860 and SGL_RS14865 are separated by 157 nucleotides SGL_RS14865 and SGL_RS14870 are separated by 5 nucleotides
SGL_RS14855: SGL_RS14855 - group I intron-associated PD-(D/E)XK endonuclease, at 2,791,074 to 2,791,526
_RS14855
SGL_RS14860: SGL_RS14860 - 8-amino-7-oxononanoate synthase, at 2,791,820 to 2,793,001
_RS14860
SGL_RS14865: SGL_RS14865 - type I methionyl aminopeptidase, at 2,793,159 to 2,793,920
_RS14865
SGL_RS14870: SGL_RS14870 - thiol-disulfide oxidoreductase DCC family protein, at 2,793,926 to 2,794,381
_RS14870
Position (kb)
2791
2792
2793
2794 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2790.872 kb on - strand at 2791.633 kb on - strand at 2791.786 kb on + strand at 2792.025 kb on - strand, within SGL_RS14860 at 2792.075 kb on - strand, within SGL_RS14860 at 2792.228 kb on - strand, within SGL_RS14860 at 2792.501 kb on - strand, within SGL_RS14860 at 2792.542 kb on + strand, within SGL_RS14860 at 2792.555 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.643 kb on + strand, within SGL_RS14860 at 2792.806 kb on + strand, within SGL_RS14860 at 2792.932 kb on + strand at 2792.946 kb on + strand at 2793.026 kb on - strand at 2793.065 kb on + strand at 2793.177 kb on - strand at 2793.231 kb on + strand at 2793.481 kb on - strand, within SGL_RS14865 at 2793.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ethanol 2 vol% remove 2,790,872 - -0.5 2,791,633 - -2.2 2,791,786 + -0.3 2,792,025 - SGL_RS14860 0.17 -2.0 2,792,075 - SGL_RS14860 0.22 -4.1 2,792,228 - SGL_RS14860 0.35 -3.5 2,792,501 - SGL_RS14860 0.58 -3.0 2,792,542 + SGL_RS14860 0.61 -0.4 2,792,555 - SGL_RS14860 0.62 -2.7 2,792,560 - SGL_RS14860 0.63 -3.2 2,792,560 - SGL_RS14860 0.63 -1.3 2,792,560 - SGL_RS14860 0.63 -3.3 2,792,643 + SGL_RS14860 0.70 -1.7 2,792,806 + SGL_RS14860 0.83 -2.3 2,792,932 + -2.3 2,792,946 + -3.0 2,793,026 - -0.0 2,793,065 + -0.5 2,793,177 - -1.1 2,793,231 + -0.4 2,793,481 - SGL_RS14865 0.42 -1.2 2,793,850 - -0.7
Or see this region's nucleotide sequence